Hl3 is a toxin isolated by our investigation group from Hadruroides lunatus scorpion venom by ionexchange chromatography on CM-Sephadex C-25 to pH 7, and which produce contraction and paralysis in the inoculated limb of white mice. In this work we have determined that inoculation of Hl3 (26 μg) in gastrocnemius muscle from mice produces the release in plasma of creatine kinase and lactate deshidrogenase. The creatine kinase activity increases from 210 UI/L to 8015 UI/L in 60 minutes, whrereas the LDH activity increases from 174 UI/L to 1697 UI/L in 90 minutes. By PAGE-SDS it, was demonstrated that incubation of Hl3 with gastrocnemius muscle isolated from white mice, in physiological solution to pH 7, produces the release of other different muscular proteins as well. In addition Hl3 can increase the calcium intramuscular 3,5 fold, after 45 minutes of action. Our results suggest that Hl3 ...

Hl3 is a toxin isolated by our investigation group from Hadruroides lunatus scorpion venom by ionexchange chromatography on CM-Sephadex C-25 to pH 7, and which produce contraction and paralysis in the inoculated limb of white mice. In this work we have determined that inoculation of Hl3 (26 μg) in gastrocnemius muscle from mice produces the release in plasma of creatine kinase and lactate deshidrogenase. The creatine kinase activity increases from 210 UI/L to 8015 UI/L in 60 minutes, whrereas the LDH activity increases from 174 UI/L to 1697 UI/L in 90 minutes. By PAGE-SDS it, was demonstrated that incubation of Hl3 with gastrocnemius muscle isolated from white mice, in physiological solution to pH 7, produces the release of other different muscular proteins as well. In addition Hl3 can increase the calcium intramuscular 3,5 fold, after 45 minutes of action. Our results suggest that Hl3 ...

The proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1, Hl2 y Hl3; which immobilize, respectively, insects (Grillus sp.), crustaceans (Porcellio laevis) and the inoculated limb of white mice. The three toxins are basic proteins and have no proteolitic or phospholipase activity. In addition, Hl3 increases the plasmatic level of creatine kinase after its inoculation in the gastrocnemius muscle of mice. By PAGE-SDS, Hl3 shows only one protein band of 12,5 KDa, while the other toxins have protein contaminants.

The proteins from the venom of the scorpion Hadruroides lunatus were separated by ionexchange chromatography on CM-Sephadex C-25 with 0,05M ammonium acetate buffer pH 7, getting six protein peaks in the process. The toxicity tests allowed the identification of three toxins that were denominated Hl1, Hl2 y Hl3; which immobilize, respectively, insects (Grillus sp.), crustaceans (Porcellio laevis) and the inoculated limb of white mice. The three toxins are basic proteins and have no proteolitic or phospholipase activity. In addition, Hl3 increases the plasmatic level of creatine kinase after its inoculation in the gastrocnemius muscle of mice. By PAGE-SDS, Hl3 shows only one protein band of 12,5 KDa, while the other toxins have protein contaminants.