Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose
Descripción del Articulo
The aim of the research was to soften rice husk by hydrothermal and alkaline pretreatments. Temperature was applied at 121 °C for three time periods (15, 30 and 45 minutes) and with NaOH in three concentrations (0,5, 1 and 1,5 %), it was stirred at 120 rpm for one hour, to adjust to pH 4,8; 20 % HCl...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2024 |
| Institución: | Universidad de Lima |
| Repositorio: | Revistas - Universidad de Lima |
| Lenguaje: | español |
| OAI Identifier: | oai:revistas.ulima.edu.pe:article/7211 |
| Enlace del recurso: | https://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211 |
| Nivel de acceso: | acceso abierto |
| Materia: | disaccharides rice hulls parboiled rice glucosidases cellulose hydrolysis enzymes disacáridos cáscara de arroz tratamiento hidrotérmico del arroz glucosidasas celulosa hidrólisis enzima |
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Production of cellobiose from the partial enzymatic hydrolysis of rice husk celluloseObtención de celobiosa a partir de la hidrólisis enzimática parcial de la celulosa de cáscara de arrozAyala Armijos, HumbertoRomero Calle, NicoleMadrid Celi, BraulioQuezada Sarmiento, RamiroTinoco Gálvez, Edgar Chavez Abad, Richarddisaccharidesrice hullsparboiled riceglucosidasescellulosehydrolysisenzymesdisacáridoscáscara de arroztratamiento hidrotérmico del arrozglucosidasascelulosahidrólisisenzimaThe aim of the research was to soften rice husk by hydrothermal and alkaline pretreatments. Temperature was applied at 121 °C for three time periods (15, 30 and 45 minutes) and with NaOH in three concentrations (0,5, 1 and 1,5 %), it was stirred at 120 rpm for one hour, to adjust to pH 4,8; 20 % HCl was added, and 30 FPU of the enzyme β-glucosidase was added and stirred at 120 rpm at 50 °C for 144 hours. The application of 121 °C for 45 minutes achieved a 34,62 ± 0,79 % partial hydrolysis of cellulose to cellobiose and 18,14 ± 0,09 % total hydrolysis of cellulose to glucose and in the hydrolysates where it was pretreated with sodium hydroxide at 1,5 %, 8,39 ± 0,79 % cellobiose and 46,64 ± 0,30 % glucose were reached. The application of hydrothermal pretreatment favors the partial hydrolysis of cellulose to cellobiose.El objetivo de la investigación fue ablandar la cáscara de arroz mediante pretratamientos hidrotérmicos y alcalinos. Se aplicó temperatura a 121 °C por tres lapsos de tiempo (15, 30 y 45 minutos) y con NaOH en tres concentraciones (0,5, 1 y 1,5 %); se agitó a 120 rpm por una hora; para ajustar a pH 4,8 se adicionó HCl al 20 %, y se añadió 30 FPU de la enzima β-glucosidasa; se agitó a 120 rpm a 50 °C durante 144 horas. La aplicación de 121 °C durante 45 minutos logró un 34,62 ± 0,79 % de hidrólisis parcial de celulosa a celobiosa y 18,14 ± 0,09 % de hidrólisis total de celulosa a glucosa; y en los hidrolizados donde se pretrató con hidróxido de sodio al 1,5 %, se alcanzó 8,39 ± 0,79 % de celobiosa y 46,64 ± 0,30 % de glucosa. La aplicación de pretratamiento hidrotérmico favorece la hidrólisis parcial de la celulosa a celobiosa. Universidad de Lima2024-12-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/721110.26439/ing.ind2024.n47.7211Ingeniería Industrial; No. 47 (2024); 129-144Ingeniería Industrial; Núm. 47 (2024); 129-1442523-63261025-992910.26439/ing.ind2024.n47reponame:Revistas - Universidad de Limainstname:Universidad de Limainstacron:ULIMAspahttps://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211/7361https://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211/7384https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:revistas.ulima.edu.pe:article/72112024-12-12T11:27:58Z |
| dc.title.none.fl_str_mv |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose Obtención de celobiosa a partir de la hidrólisis enzimática parcial de la celulosa de cáscara de arroz |
| title |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose |
| spellingShingle |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose Ayala Armijos, Humberto disaccharides rice hulls parboiled rice glucosidases cellulose hydrolysis enzymes disacáridos cáscara de arroz tratamiento hidrotérmico del arroz glucosidasas celulosa hidrólisis enzima |
| title_short |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose |
| title_full |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose |
| title_fullStr |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose |
| title_full_unstemmed |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose |
| title_sort |
Production of cellobiose from the partial enzymatic hydrolysis of rice husk cellulose |
| dc.creator.none.fl_str_mv |
Ayala Armijos, Humberto Romero Calle, Nicole Madrid Celi, Braulio Quezada Sarmiento, Ramiro Tinoco Gálvez, Edgar Chavez Abad, Richard |
| author |
Ayala Armijos, Humberto |
| author_facet |
Ayala Armijos, Humberto Romero Calle, Nicole Madrid Celi, Braulio Quezada Sarmiento, Ramiro Tinoco Gálvez, Edgar Chavez Abad, Richard |
| author_role |
author |
| author2 |
Romero Calle, Nicole Madrid Celi, Braulio Quezada Sarmiento, Ramiro Tinoco Gálvez, Edgar Chavez Abad, Richard |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
disaccharides rice hulls parboiled rice glucosidases cellulose hydrolysis enzymes disacáridos cáscara de arroz tratamiento hidrotérmico del arroz glucosidasas celulosa hidrólisis enzima |
| topic |
disaccharides rice hulls parboiled rice glucosidases cellulose hydrolysis enzymes disacáridos cáscara de arroz tratamiento hidrotérmico del arroz glucosidasas celulosa hidrólisis enzima |
| description |
The aim of the research was to soften rice husk by hydrothermal and alkaline pretreatments. Temperature was applied at 121 °C for three time periods (15, 30 and 45 minutes) and with NaOH in three concentrations (0,5, 1 and 1,5 %), it was stirred at 120 rpm for one hour, to adjust to pH 4,8; 20 % HCl was added, and 30 FPU of the enzyme β-glucosidase was added and stirred at 120 rpm at 50 °C for 144 hours. The application of 121 °C for 45 minutes achieved a 34,62 ± 0,79 % partial hydrolysis of cellulose to cellobiose and 18,14 ± 0,09 % total hydrolysis of cellulose to glucose and in the hydrolysates where it was pretreated with sodium hydroxide at 1,5 %, 8,39 ± 0,79 % cellobiose and 46,64 ± 0,30 % glucose were reached. The application of hydrothermal pretreatment favors the partial hydrolysis of cellulose to cellobiose. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-12-11 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211 10.26439/ing.ind2024.n47.7211 |
| url |
https://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211 |
| identifier_str_mv |
10.26439/ing.ind2024.n47.7211 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211/7361 https://revistas.ulima.edu.pe/index.php/Ingenieria_industrial/article/view/7211/7384 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad de Lima |
| publisher.none.fl_str_mv |
Universidad de Lima |
| dc.source.none.fl_str_mv |
Ingeniería Industrial; No. 47 (2024); 129-144 Ingeniería Industrial; Núm. 47 (2024); 129-144 2523-6326 1025-9929 10.26439/ing.ind2024.n47 reponame:Revistas - Universidad de Lima instname:Universidad de Lima instacron:ULIMA |
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Universidad de Lima |
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ULIMA |
| institution |
ULIMA |
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Revistas - Universidad de Lima |
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Revistas - Universidad de Lima |
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1841719288259411968 |
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12.851315 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
