Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri

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It was determined the effect of Lecanicillium lecanii (Zimm) and Beauveria bassiana (Bals.) On the phytophagous mite Panonychus citri. For this, the breeding of P. citri was carried out, and it was propagated to both fungi in potato sucrose agar. Five treatments were performed with three replicates...

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Autores: Erika Paola Alayo-Aguirrea, Nadiezhda Esperanza Burgos-Wilson, Juan Héctor Wilson-Krugg
Formato: artículo
Fecha de Publicación:2017
Institución:Universidad Católica de Santa María
Repositorio:Revistas - Universidad Católica de Santa María
Lenguaje:español
OAI Identifier:oai:ojs.revistas.ucsm.edu.pe:article/100
Enlace del recurso:https://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/100
Nivel de acceso:acceso abierto
Materia:Lecanicillium lecanii
Beauveria bassiana
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network_acronym_str REVUCSM
network_name_str Revistas - Universidad Católica de Santa María
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dc.title.none.fl_str_mv Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
Efecto de Lecanicillium lecanii (Zimm) y Beauveria bassiana (Bals.) sobre Panonychus citri

title Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
spellingShingle Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
Erika Paola Alayo-Aguirrea
Lecanicillium lecanii
Beauveria bassiana
title_short Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
title_full Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
title_fullStr Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
title_full_unstemmed Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
title_sort Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri
dc.creator.none.fl_str_mv Erika Paola Alayo-Aguirrea
Nadiezhda Esperanza Burgos-Wilson
Juan Héctor Wilson-Krugg
author Erika Paola Alayo-Aguirrea
author_facet Erika Paola Alayo-Aguirrea
Nadiezhda Esperanza Burgos-Wilson
Juan Héctor Wilson-Krugg
author_role author
author2 Nadiezhda Esperanza Burgos-Wilson
Juan Héctor Wilson-Krugg
author2_role author
author
dc.subject.none.fl_str_mv Lecanicillium lecanii
Beauveria bassiana
topic Lecanicillium lecanii
Beauveria bassiana
description It was determined the effect of Lecanicillium lecanii (Zimm) and Beauveria bassiana (Bals.) On the phytophagous mite Panonychus citri. For this, the breeding of P. citri was carried out, and it was propagated to both fungi in potato sucrose agar. Five treatments were performed with three replicates each; each treatment had 30 specimens between nymphs and adults of P. citri and the technique of inoculation was by spray. At the first treatment, 0.1% tween 80 was inoculated; at the second and third treatments, L. lecanii were applied at concentrations of 106 and 107 conidia / mL respectively; and at the fourth and fifth treatments, B. bassiana was applied at 106 and 107 conidia / mL respectively, and the specimens were incubated at room temperature until symptoms appeared to be slow moving, tegument darkening, dehydration and death. Dead mites were placed in a humid chamber until the appearance of mycelium, which was isolated on sucrose potato agar with antibiotic for microscopic observation and identification. It was found that the lowest percentage of P. citri survival was compared to B. bassiana with 17% and 6% at the concentrations of 106 con / mL and 107 con / mL, with a significant difference; Whereas with L. lecanii the survival rate was 29% and 20% at the concentrations of 106 con/ mL and 107 con/ mL, with no significant difference between the two concentrations. It was concluded that B. bassiana and L. lecanii have an entomopathogenic effect on the nymphs and adults of P. citri; obtaining a lower percentage of mite survival by increasing the concentration of B. bassiana conidia while in L. lecanii the two concentrations of conidia have the same entomopathogenic effect on the nymphs and adults of P. citri
publishDate 2017
dc.date.none.fl_str_mv 2017-09-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/100
10.35286/veritas.v16i1.100
url https://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/100
identifier_str_mv 10.35286/veritas.v16i1.100
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/100/94
dc.rights.none.fl_str_mv Derechos de autor 2017 Veritas
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2017 Veritas
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Católica de Santa María
publisher.none.fl_str_mv Universidad Católica de Santa María
dc.source.none.fl_str_mv Veritas; Vol. 16 Núm. 1 (2015): VÉRITAS: Investigación, Innovación y Desarrollo; 73-77
Veritas; Vol. 16 Núm. 1 (2015): VÉRITAS: Investigación, Innovación y Desarrollo; 73-77
Veritas; Vol. 16 Núm. 1 (2015): VÉRITAS: Investigación, Innovación y Desarrollo; 73-77
1684-7822
1684-7822
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spelling Effect of Lecanicillium lecanii (zimm) and Beauveria bassiana (bals.) About Panonychus citri Efecto de Lecanicillium lecanii (Zimm) y Beauveria bassiana (Bals.) sobre Panonychus citriErika Paola Alayo-AguirreaNadiezhda Esperanza Burgos-WilsonJuan Héctor Wilson-KruggLecanicillium lecaniiBeauveria bassianaIt was determined the effect of Lecanicillium lecanii (Zimm) and Beauveria bassiana (Bals.) On the phytophagous mite Panonychus citri. For this, the breeding of P. citri was carried out, and it was propagated to both fungi in potato sucrose agar. Five treatments were performed with three replicates each; each treatment had 30 specimens between nymphs and adults of P. citri and the technique of inoculation was by spray. At the first treatment, 0.1% tween 80 was inoculated; at the second and third treatments, L. lecanii were applied at concentrations of 106 and 107 conidia / mL respectively; and at the fourth and fifth treatments, B. bassiana was applied at 106 and 107 conidia / mL respectively, and the specimens were incubated at room temperature until symptoms appeared to be slow moving, tegument darkening, dehydration and death. Dead mites were placed in a humid chamber until the appearance of mycelium, which was isolated on sucrose potato agar with antibiotic for microscopic observation and identification. It was found that the lowest percentage of P. citri survival was compared to B. bassiana with 17% and 6% at the concentrations of 106 con / mL and 107 con / mL, with a significant difference; Whereas with L. lecanii the survival rate was 29% and 20% at the concentrations of 106 con/ mL and 107 con/ mL, with no significant difference between the two concentrations. It was concluded that B. bassiana and L. lecanii have an entomopathogenic effect on the nymphs and adults of P. citri; obtaining a lower percentage of mite survival by increasing the concentration of B. bassiana conidia while in L. lecanii the two concentrations of conidia have the same entomopathogenic effect on the nymphs and adults of P. citriSe evaluó el efecto de Lecanicillium lecanii (Zimm) y Beauveria bassiana (Bals.) sobre el ácaro fitó- fago Panonychus citri. Para esto se realizó la crianza de P. citri, y se propago a ambos hongos en agar papa sacarosa. Se realizaron cinco tratamientos con tres repeticiones cada uno; teniendo cada tratamiento 30 especímenes entre ninfas y adultos de P. citri y la técnica de inoculación fue por aspersión. Al primer tratamiento se inoculó tween 80 al 0.1%, al segundo y tercer tratamiento se les aplicó L. lecanii a las concentraciones de 106 y 107 conidias/mL respectivamente; y al cuarto y quinto tratamiento se les aplicó B. bassiana a las concentraciones de 106 y 107 conidias/mL respectivamente, incubándose los especímenes a temperatura ambiente hasta la aparición de síntomas como lentitud en movimiento, oscurecimiento del tegumento, deshidratación y muerte. Los ácaros muertos se colocaron en cámara húmeda hasta la aparición de micelio, el cuál fue aislado en agar papa sacarosa con antibiótico para su observación microscópica e identificación. Se encontró que el menor porcentaje de supervivencia de P. citri fue frente a B. bassiana con un 17% y 6% a las concentraciones de 106 con/mL y 107 con/mL, existiendo diferencia significativa; mientras que con L. lecanii el porcentaje de supervivencia fue de 29% y 20% a las concentraciones de 106 con/mL y 107 con/mL, no existiendo diferencia significativa entre ambas concentraciones. Se concluye que B. bassiana y L. lecanii tienen efecto entomopatógeno sobre las ninfas y adultos de P. citri; obteniéndose un menor porcentaje de supervivencia del ácaro al incrementarse la concentración de conidias de B. bassiana mientras que en L. lecanii las dos concentraciones de conidias tienen el mismo efecto entomopatógeno sobre las ninfas y adultos de P. citri.Universidad Católica de Santa María2017-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/10010.35286/veritas.v16i1.100Veritas; Vol. 16 Núm. 1 (2015): VÉRITAS: Investigación, Innovación y Desarrollo; 73-77Veritas; Vol. 16 Núm. 1 (2015): VÉRITAS: Investigación, Innovación y Desarrollo; 73-77Veritas; Vol. 16 Núm. 1 (2015): VÉRITAS: Investigación, Innovación y Desarrollo; 73-771684-78221684-7822reponame:Revistas - Universidad Católica de Santa Maríainstname:Universidad Católica de Santa Maríainstacron:UCSMspahttps://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/100/94Derechos de autor 2017 Veritasinfo:eu-repo/semantics/openAccessoai:ojs.revistas.ucsm.edu.pe:article/1002022-07-19T06:04:35Z
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