Regeneration and rooting of etiolated adventitious shoots of blackberry (Rubus sp.) cultivars

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The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of anin vitropropagation protocol of threeRubussp. cultivars. Zygotic embryos were extracted from botanical seeds ofRubussp. cultivars andplaced in establishment culture medium. After...

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Detalles Bibliográficos
Autores: Millones Chanamé, Carlos Eduardo, Vásquez Castro, Ernestina Rosario
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional del Altiplano
Repositorio:Revista de Investigaciones Altoandinas
Lenguaje:español
OAI Identifier:oai:huajsapata.unap.edu.pe:article/195
Nivel de acceso:acceso abierto
Materia:Rubus
regeneration
propagation
darkness
regeneración
propagación
oscuridad
Descripción
Sumario:The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of anin vitropropagation protocol of threeRubussp. cultivars. Zygotic embryos were extracted from botanical seeds ofRubussp. cultivars andplaced in establishment culture medium. After 90 days ofin vitroculture, the seedlings were conditioned to obtain the basal stemsegments and placed in growth and development culture media, they were then subjected to periods of darkness for etiolated adventitiousshoot regeneration. Afterwards, the nodal segments were transferred to rooting culture media. Results showed that the regeneration ofadventitious shoots fromRubussp. cultivars was on average eight days. Likewise, the sectioned nodal segments of the adventitious shootsinduced roots when the medium contained the combination of the auxins NAA and IBA. The regeneration of etiolated adventitious shootsin the three cultivars ofRubussp. allowed to obtain elongated shoots in a short time and without the use of growth regulators, facilitatingthein vitropropagation of this species
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