The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of anin vitropropagation protocol of threeRubussp. cultivars. Zygotic embryos were extracted from botanical seeds ofRubussp. cultivars andplaced in establishment culture medium. After 90 days ofin vitroculture, the seedlings were conditioned to obtain the basal stemsegments and placed in growth and development culture media, they were then subjected to periods of darkness for etiolated adventitiousshoot regeneration. Afterwards, the nodal segments were transferred to rooting culture media. Results showed that the regeneration ofadventitious shoots fromRubussp. cultivars was on average eight days. Likewise, the sectioned nodal segments of the adventitious shootsinduced roots when the medium contained the combination of the auxins NAA and IBA. The regeneration of etiolated a...

The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of anin vitropropagation protocol of threeRubussp. cultivars. Zygotic embryos were extracted from botanical seeds ofRubussp. cultivars andplaced in establishment culture medium. After 90 days ofin vitroculture, the seedlings were conditioned to obtain the basal stemsegments and placed in growth and development culture media, they were then subjected to periods of darkness for etiolated adventitiousshoot regeneration. Afterwards, the nodal segments were transferred to rooting culture media. Results showed that the regeneration ofadventitious shoots fromRubussp. cultivars was on average eight days. Likewise, the sectioned nodal segments of the adventitious shootsinduced roots when the medium contained the combination of the auxins NAA and IBA. The regeneration of etiolated a...

The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of anin vitropropagation protocol of threeRubussp. cultivars. Zygotic embryos were extracted from botanical seeds ofRubussp. cultivars andplaced in establishment culture medium. After 90 days ofin vitroculture, the seedlings were conditioned to obtain the basal stemsegments and placed in growth and development culture media, they were then subjected to periods of darkness for etiolated adventitiousshoot regeneration. Afterwards, the nodal segments were transferred to rooting culture media. Results showed that the regeneration ofadventitious shoots fromRubussp. cultivars was on average eight days. Likewise, the sectioned nodal segments of the adventitious shootsinduced roots when the medium contained the combination of the auxins NAA and IBA. The regeneration of etiolated a...

Improving the efficiency in vitro culture conditions in established yellow pitahaya micropropagation protocols, allows evaluating the dependence of sucrose in the culture medium under photoautotrophic conditions to obtain high quality seedlings in less time, which favors their acclimatization in the mass production of this species. The objective of this research was to evaluate the levels of light intensity and the use or not of sucrose in the culture medium on the in vitro morphological and physiological response of yellow pitahaya (S. megalanthus) seedlings. Vegetative sections of yellow pitahaya were placed in growth and development culture media to obtain cuttings approximately six centimeters long to obtain the explants. The containers were placed at different light intensities: 50, 100 and 150 μmol/m-2s-1, using compact fluorescent lamps, whose intensities were adjusted using a lu...

El objetivo de la presente investigación fue evaluar la regeneración de brotes adventicios etiolados para el desarrollo de un protocolo de la propagación in vitro de tres cultivares de Rubus sp. Embriones cigóticos fueron extraídos de semillas botánicas de los cultivares de Rubus sp. y colocados en medio de cultivo de establecimiento. A los 90 días de cultivo in vitrolas plántulas fueron acondicionadas para obtener los segmentos basales caulinares y colocadas en medios de crecimiento y desarrollo, luego fueron sometidos a periodos de oscuridad para la regeneración de brotes adventicios etiolados. Posteriormente, los segmentos nodales fueron transferidos en medios de cultivo de enraizamiento. Los resultados mostraron que la regeneración de los brotes adventicios en los cultivares de Rubus sp. fue en promedio de ocho días, asimismo, los segmentos nodales seccionados de los brote...

The objective of the present study was to evaluate the etiolated adventitious shoot regeneration for the development of anin vitropropagation protocol of threeRubussp. cultivars. Zygotic embryos were extracted from botanical seeds ofRubussp. cultivars andplaced in establishment culture medium. After 90 days ofin vitroculture, the seedlings were conditioned to obtain the basal stemsegments and placed in growth and development culture media, they were then subjected to periods of darkness for etiolated adventitiousshoot regeneration. Afterwards, the nodal segments were transferred to rooting culture media. Results showed that the regeneration ofadventitious shoots fromRubussp. cultivars was on average eight days. Likewise, the sectioned nodal segments of the adventitious shootsinduced roots when the medium contained the combination of the auxins NAA and IBA. The regeneration of etiolated a...

Improving the efficiency in vitro culture conditions in established yellow pitahaya micropropagation protocols, allows evaluating the dependence of sucrose in the culture medium under photoautotrophic conditions to obtain high quality seedlings in less time, which favors their acclimatization in the mass production of this species. The objective of this research was to evaluate the levels of light intensity and the use or not of sucrose in the culture medium on the in vitro morphological and physiological response of yellow pitahaya (S. megalanthus) seedlings. Vegetative sections of yellow pitahaya were placed in growth and development culture media to obtain cuttings approximately six centimeters long to obtain the explants. The containers were placed at different light intensities: 50, 100 and 150 μmol/m-2s-1, using compact fluorescent lamps, whose intensities were adjusted using a lu...

El estrés salino es uno de los principales problemas que restringe la producción de los cultivos, afectando el desempeño agronómico y la calidad del fruto, por lo que la presente investigación se realizó para identificar la tolerancia a la salinidad en cultivares de Rubus spp. en la etapa vegetativa bajo condiciones in vitro. Secciones nodales de tres cultivares fueron colocadas en medio de crecimiento y desarrollo suplementado con tres niveles de NaCl (0, 35 y 70 mM). Se empleó un diseño completamente al azar con arreglo factorial (Factor A: tres cultivares y Factor B: dos niveles de NaCl y un control), con tres repeticiones. El cultivar ‘Brazos’ registró una adecuada respuesta en la altura de brote cuando fue sometido a estrés salino simulado con 70 mM de NaCl. Los cultivares ‘Tupy’ y ‘Brazos’ registraron tolerancia a 35 mM de NaCl en la variable número de hojas ...

Since the first publications on the study of microorganisms in composting, the progress has been impressive, because microbiological and molecular biological methods have been used to identify the structure of microbial communities during the process. The interaction of physico-chemical parameters and the type of composting system determine variations in the diversity and abundance of microbial species. The metabolic activity of mainly bacteria and fungi allows the degradation of organic matter and contributes to the maturation of compost, the main product of aerobic composting. In this process, bacteria are the most abundant group, comprising the Phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, followed by fungi, mainly the phylum Ascomycota. The interest in the inoculation of compost with exogenous microorganisms is increasing, due to the benefits they provide thanks...

Since the first publications on the study of microorganisms in composting, the progress has been impressive, because microbiological and molecular biological methods have been used to identify the structure of microbial communities during the process. The interaction of physico-chemical parameters and the type of composting system determine variations in the diversity and abundance of microbial species. The metabolic activity of mainly bacteria and fungi allows the degradation of organic matter and contributes to the maturation of compost, the main product of aerobic composting. In this process, bacteria are the most abundant group, comprising the Phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, followed by fungi, mainly the phylum Ascomycota. The interest in the inoculation of compost with exogenous microorganisms is increasing, due to the benefits they provide thanks...

El objetivo de esta investigación fue inducir bulbillos de azucena (Lilium sp.) a partir de escamas empleando reguladores de crecimiento. En la etapa de inducción de bulbillos se emplearon bulbos de azucena de dos calibres, asimismo 100 mg/L de ácido naftalenacético (ANA), 50 y 100 mg/L de bencilaminopurina (BAP), solos o combinados. Los bulbos fueron desinfectados y sometidos a los reguladores de crecimiento, y las escamas colocadas en sustrato PREMIX a capacidad de campo. En la etapa de establecimiento se emplearon bulbillos inducidos y colocados en sustrato para el crecimiento y desarrollo de plántulas. Los resultados mostraron que el empleo de 100 mg/L ANA, 100 mg/L de BAP, solos o combinados permitieron inducir un mayor número de bulbillos a partir de escamas de bulbos con mayor calibre en comparación con el testigo. En la etapa de establecimiento se determinó que los bulbil...

Los campos magnéticos (CMs) son un factor ambiental necesario para todos los seres vivos. En particular el crecimiento y desarrollo de las plántulas in vitro, los CMs pueden ser una alternativa viable en la propagación in vitro de especies de Rubus spp. El presente trabajo evaluó la influencia de los CMs en la respuesta morfogénica in vitro de plántulas de especies silvestres (ES) de Rubus spp. Se instalaron dos experimentos; el primer experimento evaluó el efecto de intensidad de los CMs sobre la respuesta morfogénica en los segmentos nodales, empleando dos intensidades 100 y 200 mT comparadas con el control (0 mT); el segundo experimento evaluó el efecto de la duración de la exposición del CM de 200 mT en cuatro periodos: 0, 7, 14 y 21 días. Ambos experimentos permitieron identificar el efecto de los CMs en la respuesta morfogénica como la longitud de brotes e inducción d...

Los campos magnéticos (CMs) son un factor ambiental necesario para todos los seres vivos. En particular el crecimiento y desarrollo de las plántulas in vitro, los CMs pueden ser una alternativa viable en la propagación in vitro de especies de Rubus spp. El presente trabajo evaluó la influencia de los CMs en la respuesta morfogénica in vitro de plántulas de especies silvestres (ES) de Rubus spp. Se instalaron dos experimentos; el primer experimento evaluó el efecto de intensidad de los CMs sobre la respuesta morfogénica en los segmentos nodales, empleando dos intensidades 100 y 200 mT comparadas con el control (0 mT); el segundo experimento evaluó el efecto de la duración de la exposición del CM de 200 mT en cuatro periodos: 0, 7, 14 y 21 días. Ambos experimentos permitieron identificar el efecto de los CMs en la respuesta morfogénica como la longitud de brotes e inducción d...