Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance

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9 Páginas
Detalles Bibliográficos
Autores: Gutiérrez Calle, Savina Alejandra, Sánchez Díaz, Rosa A., Delgado Silva, Yolanda Bedsabé, Montenegro, Juan D., Gutiérrez Reynoso, Dina Lida, Maicelo Quintana, Jorge Luis, Guerrero Abad, Juan Carlos
Formato: artículo
Fecha de Publicación:2021
Institución:Instituto Nacional de Innovación Agraria
Repositorio:INIA-Institucional
Lenguaje:inglés
OAI Identifier:oai:null:20.500.12955/1301
Enlace del recurso:https://repositorio.inia.gob.pe/handle/20.500.12955/1301
https://doi.org/10.17268/sci.agropecu.2021.011
Nivel de acceso:acceso abierto
Materia:Coffee
Capillary
Electrophoresis
Hemileia vastatrix
SH3
Biotecnología agrícola, Biotecnología alimentaria
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dc.title.es_PE.fl_str_mv Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
title Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
spellingShingle Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
Gutiérrez Calle, Savina Alejandra
Coffee
Capillary
Electrophoresis
Hemileia vastatrix
SH3
Biotecnología agrícola, Biotecnología alimentaria
title_short Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
title_full Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
title_fullStr Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
title_full_unstemmed Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
title_sort Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
author Gutiérrez Calle, Savina Alejandra
author_facet Gutiérrez Calle, Savina Alejandra
Sánchez Díaz, Rosa A.
Delgado Silva, Yolanda Bedsabé
Montenegro, Juan D.
Gutiérrez Reynoso, Dina Lida
Maicelo Quintana, Jorge Luis
Guerrero Abad, Juan Carlos
author_role author
author2 Sánchez Díaz, Rosa A.
Delgado Silva, Yolanda Bedsabé
Montenegro, Juan D.
Gutiérrez Reynoso, Dina Lida
Maicelo Quintana, Jorge Luis
Guerrero Abad, Juan Carlos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gutiérrez Calle, Savina Alejandra
Sánchez Díaz, Rosa A.
Delgado Silva, Yolanda Bedsabé
Montenegro, Juan D.
Gutiérrez Reynoso, Dina Lida
Maicelo Quintana, Jorge Luis
Guerrero Abad, Juan Carlos
dc.subject.es_PE.fl_str_mv Coffee
Capillary
Electrophoresis
Hemileia vastatrix
SH3
topic Coffee
Capillary
Electrophoresis
Hemileia vastatrix
SH3
Biotecnología agrícola, Biotecnología alimentaria
dc.subject.ocde.es_PE.fl_str_mv Biotecnología agrícola, Biotecnología alimentaria
description 9 Páginas
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-03-15T18:30:46Z
dc.date.available.none.fl_str_mv 2021-03-15T18:30:46Z
dc.date.issued.fl_str_mv 2021-03-10
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.es_PE.fl_str_mv Gutiérrez-Calle, S. A. ., Sánchez-Díaz, R. A. ., Delgado-Silva, Y. B. ., Montenegro, J. D. ., Gutiérrez, D. L. ., Maicelo-Quintana, J. L. ., & Guerrero-Abad, J. C. . (2021). Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance. Scientia Agropecuaria, 12(1), 91-99. doi: 10.17268/sci.agropecu.2021.011
dc.identifier.uri.none.fl_str_mv https://repositorio.inia.gob.pe/handle/20.500.12955/1301
dc.identifier.journal.es_PE.fl_str_mv Scientia Agropecuaria
dc.identifier.doi.none.fl_str_mv https://doi.org/10.17268/sci.agropecu.2021.011
identifier_str_mv Gutiérrez-Calle, S. A. ., Sánchez-Díaz, R. A. ., Delgado-Silva, Y. B. ., Montenegro, J. D. ., Gutiérrez, D. L. ., Maicelo-Quintana, J. L. ., & Guerrero-Abad, J. C. . (2021). Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance. Scientia Agropecuaria, 12(1), 91-99. doi: 10.17268/sci.agropecu.2021.011
Scientia Agropecuaria
url https://repositorio.inia.gob.pe/handle/20.500.12955/1301
https://doi.org/10.17268/sci.agropecu.2021.011
dc.language.iso.es_PE.fl_str_mv eng
language eng
dc.relation.ispartof.es_PE.fl_str_mv Scientia Agropecuaria. Vol. 12 Núm. 1 (2021). 91-99
dc.relation.publisherversion.es_PE.fl_str_mv https://doi.org/10.17268/sci.agropecu.2021.011
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dc.format.es_PE.fl_str_mv application/pdf
dc.coverage.spatial.es_PE.fl_str_mv Perú
dc.publisher.es_PE.fl_str_mv Universidad Nacional de Trujillo
dc.publisher.country.es_PE.fl_str_mv Perú
dc.source.es_PE.fl_str_mv Instituto Nacional de Innovación Agraria
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spelling Gutiérrez Calle, Savina AlejandraSánchez Díaz, Rosa A.Delgado Silva, Yolanda BedsabéMontenegro, Juan D.Gutiérrez Reynoso, Dina LidaMaicelo Quintana, Jorge LuisGuerrero Abad, Juan CarlosPerú2021-03-15T18:30:46Z2021-03-15T18:30:46Z2021-03-10Gutiérrez-Calle, S. A. ., Sánchez-Díaz, R. A. ., Delgado-Silva, Y. B. ., Montenegro, J. D. ., Gutiérrez, D. L. ., Maicelo-Quintana, J. L. ., & Guerrero-Abad, J. C. . (2021). Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance. Scientia Agropecuaria, 12(1), 91-99. doi: 10.17268/sci.agropecu.2021.011https://repositorio.inia.gob.pe/handle/20.500.12955/1301Scientia Agropecuariahttps://doi.org/10.17268/sci.agropecu.2021.0119 PáginasCoffee is an important agricultural commodity in the world. However, it is susceptible to Hemileia vastatrix (Hv), an obligatory biotrophic fungus that causes coffee leaf rust (CLR). Natural resistance to rust has been identified in the wild species Coffea canephora and Coffea liberica. These species have been used in breeding programs where interspecific resistant hybrids have been generated. The SH3 gene, derived from C. liberica, has been shown to confer extreme and long-lasting resistance to Hv. A total of 167 accessions of the INIA’s Coffee Germplasm Collection of Peru (INIA-CGC) were screened with 4 markers linked to the SH3 gene. As positive controls, EA67 (C. liberica) and the hybrid S.288 (C. arabica x C. liberica) were used. Separation of PCR products was done by capillary electrophoresis, which allow to discriminate the alleles of each marker. For three markers, specific alleles for either C. arabica or C. liberica species were found. In all cases, S.288 exhibited specific alleles for both species; whereas the INIA-CGC accessions had exclusively C. arabica alleles and EA67 had C. liberica alleles. The BA-48-21O-f marker did not produce PCR fragments for any of the positive controls, suggesting that this marker is not as predictive as the other three to determine the presence of SH3. This work reports the existence of multiple alleles for the Sat244 marker; however, the collection does not have the SH3 mediated-resistance gene. Finally, the utility of capillary electrophoresis as a tool to identify alleles linked to SH3 was demonstrated.1. Introduction. 2. Materials and methods. 3. Results and discussion. 4. Conclusions. References.application/pdfengUniversidad Nacional de TrujilloPerúScientia Agropecuaria. 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Capillary electrophoresis as a tool for genotyping SH3 mediated coffee leaf rust resistance
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