Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas
Descripción del Articulo
The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentati...
| Autores: | , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2019 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | español |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/1287 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/1287 https://doi.org/10.17843/rpmesp.2019.362.4151 |
| Nivel de acceso: | acceso abierto |
| Materia: | Sedimentación en tubo Giardia spp. Sacarosa Gradiente de densidad Recuento de células https://purl.org/pe-repo/ocde/ford#3.01.08 |
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CONC_c4749640c0ae8ad0de565b0541500eb5 |
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oai:repositorio.concytec.gob.pe:20.500.12390/1287 |
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4689 |
| dc.title.none.fl_str_mv |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| title |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| spellingShingle |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas Tarqui Terrones, Kathia Sedimentación en tubo Giardia spp. Sacarosa Gradiente de densidad Recuento de células https://purl.org/pe-repo/ocde/ford#3.01.08 |
| title_short |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| title_full |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| title_fullStr |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| title_full_unstemmed |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| title_sort |
Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas |
| author |
Tarqui Terrones, Kathia |
| author_facet |
Tarqui Terrones, Kathia Ramirez Carranza, Giovanna Beltrán Fabián, Maria |
| author_role |
author |
| author2 |
Ramirez Carranza, Giovanna Beltrán Fabián, Maria |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Tarqui Terrones, Kathia Ramirez Carranza, Giovanna Beltrán Fabián, Maria |
| dc.subject.none.fl_str_mv |
Sedimentación en tubo |
| topic |
Sedimentación en tubo Giardia spp. Sacarosa Gradiente de densidad Recuento de células https://purl.org/pe-repo/ocde/ford#3.01.08 |
| dc.subject.es_PE.fl_str_mv |
Giardia spp. Sacarosa Gradiente de densidad Recuento de células |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#3.01.08 |
| description |
The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp. |
| publishDate |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2019-06-25 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/1287 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.17843/rpmesp.2019.362.4151 |
| url |
https://hdl.handle.net/20.500.12390/1287 https://doi.org/10.17843/rpmesp.2019.362.4151 |
| dc.language.iso.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.ispartof.none.fl_str_mv |
Revista Peruana de Medicina Experimental y Salud Pública |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Instituto Nacional de Salud (Peru) |
| publisher.none.fl_str_mv |
Instituto Nacional de Salud (Peru) |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
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Consejo Nacional de Ciencia Tecnología e Innovación |
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CONCYTEC |
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CONCYTEC |
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CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1839175818948902912 |
| spelling |
Publicationrp03739600rp03740600rp03738600Tarqui Terrones, KathiaRamirez Carranza, GiovannaBeltrán Fabián, Maria2024-05-30T23:13:38Z2024-05-30T23:13:38Z2019-06-25https://hdl.handle.net/20.500.12390/1287https://doi.org/10.17843/rpmesp.2019.362.4151The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecspaInstituto Nacional de Salud (Peru)Revista Peruana de Medicina Experimental y Salud Públicainfo:eu-repo/semantics/openAccessSedimentación en tuboGiardia spp.-1Sacarosa-1Gradiente de densidad-1Recuento de células-1https://purl.org/pe-repo/ocde/ford#3.01.08-1Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicasinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/1287oai:repositorio.concytec.gob.pe:20.500.12390/12872024-05-30 16:02:21.924http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="f3b6e044-c902-4bdf-ad0c-464e459d2562"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>spa</Language> <Title>Evaluación de métodos de concentración y purificación de Giardia spp. a partir de muestras coprológicas</Title> <PublishedIn> <Publication> <Title>Revista Peruana de Medicina Experimental y Salud Pública</Title> </Publication> </PublishedIn> <PublicationDate>2019-06-25</PublicationDate> <DOI>https://doi.org/10.17843/rpmesp.2019.362.4151</DOI> <Authors> <Author> <DisplayName>Tarqui Terrones, Kathia</DisplayName> <Person id="rp03739" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ramirez Carranza, Giovanna</DisplayName> <Person id="rp03740" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Beltrán Fabián, Maria</DisplayName> <Person id="rp03738" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Instituto Nacional de Salud (Peru)</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Sedimentación en tubo</Keyword> <Keyword>Giardia spp.</Keyword> <Keyword>Sacarosa</Keyword> <Keyword>Gradiente de densidad</Keyword> <Keyword>Recuento de células</Keyword> <Abstract>The aim of this study was to compare different methods of concentration to recover the largest number of Giardia spp. cysts from coprological samples. One hundred (100) samples from national reference hospitals were analyzed and four parasitological methods were applied: spontaneous tube sedimentation concentration (TSET), Faust, single-phase sucrose gradient, and two-phase sucrose gradient. The two-phase sucrose gradient method was found to achieve significantly better results in cyst concentration (121,903 cysts/ml) and amount of debris (6%), compared to Faust methods (35,355 cysts/ml), spontaneous tube sedimentation concentration (20,145 cysts/ml), and single-phase sucrose gradient (18,702 cysts/ml). It is concluded that the most effective method for the concentration and purification of Giardia spp. cysts from coprological samples is the two-phase sucrose gradient method, which would facilitate in vitro culture of Giardia spp.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
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13.461011 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
