EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
Descripción del Articulo
Micrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial proper...
Autores: | , , , , |
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Formato: | artículo |
Fecha de Publicación: | 2021 |
Institución: | Universidad Ricardo Palma |
Repositorio: | Revista URP - Biotempo |
Lenguaje: | español |
OAI Identifier: | oai:oai.revistas.urp.edu.pe:article/3950 |
Enlace del recurso: | http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950 |
Nivel de acceso: | acceso abierto |
Materia: | Micrococcus luteus carotenoide pigmento antimicrobiano |
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EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUSEXTRACCIÓN, CARACTERIZACIÓN Y ACTIVIDAD ANTIMICROBIANA DEL PIGMENTO CAROTENOIDE DE MICROCOCCUS LUTEUSFernández-Sánchez, HarleyAgurto-Sáenz, TomásGonzales Molfino, Hugo MauricioChavieri-Salazar, Andrés RicardoGuerra-Santa Cruz, AlcidesMicrococcus luteuscarotenoidepigmentoantimicrobianoMicrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial properties that may depend on the origin of isolation. Currently, it is important to explore several food grade pigments from natural sources and their potential effects on the food industry, human or veterinary health since they tend to be less harmful. For that reason, the first objective of the study was to extract this carotenoid pigment from M. luteus isolated from the environment with volatile organic solvents. Second, to characterize this pigment using a Thin Layer Chromatography technique. Finally, to perform an antimicrobial essay testing some of most common pathogens in human and animal health all provided by the Ricardo Palma University Microbiology Laboratory, Lima, Peru. Results included not only a successful and simplified crude extraction of the carotenoid pigment, but probed its antibacterial activity against some pathogens strains such as V. parahaemolyticus, P. fluorescens and R. equi.Micrococcus luteus (Schroeter) Cohn 1872 es una de las bacterias que se encuentran en el ambiente, sus colonias presentan una pigmentación amarillenta. Este pigmento es un carotenoide cuyas propiedades varían según el ambiente de origen o aislamiento. En la actualidad, es importante explorar varias fuentes naturales de colorantes de grado alimenticio y sus potenciales efectos en la industria alimentaria, la salud humana o veterinaria. Por ello, el presente trabajo tuvo como objetivo realizar la extracción del pigmento carotenoide de M. luteus con solventes orgánicos y la respectiva caracterización mediante cromatografía en capa fina (TLC) del pigmento carotenoide de M. luteus aislado del Laboratorio de Microbiología de la Universidad Ricardo Palma, Lima, Perú, para, posteriormente, evaluar su actividad antimicrobiana frente a una serie de cepas proporcionadas por este mismo laboratorio. Consecuentemente, se obtuvo no solo una extracción simplificada cruda del pigmento con étil acetato y un factor de retención (R/ f) de 0.4 para ese pigmento, sino que también se confirmó su actividad antibacterial contra algunas cepas patógenas de importancia humana y veterinaria como lo son V. parahaemolyticus, P. fluorescens y R. equi.Facultad de Ciencias Biológicas, Universidad Ricardo Palma2021-06-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistas.urp.edu.pe/index.php/Biotempo/article/view/395010.31381/biotempo.v18i1.3950Biotempo; Vol. 18 Núm. 1 (2021): Biotempo Journal Manuscript accepted, early view2519-56971992-2159reponame:Revista URP - Biotempoinstname:Universidad Ricardo Palmainstacron:URPspahttp://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950/4789Derechos de autor 2021 Biotempoinfo:eu-repo/semantics/openAccess2021-06-04T16:20:16Zmail@mail.com - |
dc.title.none.fl_str_mv |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS EXTRACCIÓN, CARACTERIZACIÓN Y ACTIVIDAD ANTIMICROBIANA DEL PIGMENTO CAROTENOIDE DE MICROCOCCUS LUTEUS |
title |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS |
spellingShingle |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS Fernández-Sánchez, Harley Micrococcus luteus carotenoide pigmento antimicrobiano |
title_short |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS |
title_full |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS |
title_fullStr |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS |
title_full_unstemmed |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS |
title_sort |
EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS |
dc.creator.none.fl_str_mv |
Fernández-Sánchez, Harley Agurto-Sáenz, Tomás Gonzales Molfino, Hugo Mauricio Chavieri-Salazar, Andrés Ricardo Guerra-Santa Cruz, Alcides |
author |
Fernández-Sánchez, Harley |
author_facet |
Fernández-Sánchez, Harley Agurto-Sáenz, Tomás Gonzales Molfino, Hugo Mauricio Chavieri-Salazar, Andrés Ricardo Guerra-Santa Cruz, Alcides |
author_role |
author |
author2 |
Agurto-Sáenz, Tomás Gonzales Molfino, Hugo Mauricio Chavieri-Salazar, Andrés Ricardo Guerra-Santa Cruz, Alcides |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Micrococcus luteus carotenoide pigmento antimicrobiano |
topic |
Micrococcus luteus carotenoide pigmento antimicrobiano |
dc.description.none.fl_txt_mv |
Micrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial properties that may depend on the origin of isolation. Currently, it is important to explore several food grade pigments from natural sources and their potential effects on the food industry, human or veterinary health since they tend to be less harmful. For that reason, the first objective of the study was to extract this carotenoid pigment from M. luteus isolated from the environment with volatile organic solvents. Second, to characterize this pigment using a Thin Layer Chromatography technique. Finally, to perform an antimicrobial essay testing some of most common pathogens in human and animal health all provided by the Ricardo Palma University Microbiology Laboratory, Lima, Peru. Results included not only a successful and simplified crude extraction of the carotenoid pigment, but probed its antibacterial activity against some pathogens strains such as V. parahaemolyticus, P. fluorescens and R. equi. Micrococcus luteus (Schroeter) Cohn 1872 es una de las bacterias que se encuentran en el ambiente, sus colonias presentan una pigmentación amarillenta. Este pigmento es un carotenoide cuyas propiedades varían según el ambiente de origen o aislamiento. En la actualidad, es importante explorar varias fuentes naturales de colorantes de grado alimenticio y sus potenciales efectos en la industria alimentaria, la salud humana o veterinaria. Por ello, el presente trabajo tuvo como objetivo realizar la extracción del pigmento carotenoide de M. luteus con solventes orgánicos y la respectiva caracterización mediante cromatografía en capa fina (TLC) del pigmento carotenoide de M. luteus aislado del Laboratorio de Microbiología de la Universidad Ricardo Palma, Lima, Perú, para, posteriormente, evaluar su actividad antimicrobiana frente a una serie de cepas proporcionadas por este mismo laboratorio. Consecuentemente, se obtuvo no solo una extracción simplificada cruda del pigmento con étil acetato y un factor de retención (R/ f) de 0.4 para ese pigmento, sino que también se confirmó su actividad antibacterial contra algunas cepas patógenas de importancia humana y veterinaria como lo son V. parahaemolyticus, P. fluorescens y R. equi. |
description |
Micrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial properties that may depend on the origin of isolation. Currently, it is important to explore several food grade pigments from natural sources and their potential effects on the food industry, human or veterinary health since they tend to be less harmful. For that reason, the first objective of the study was to extract this carotenoid pigment from M. luteus isolated from the environment with volatile organic solvents. Second, to characterize this pigment using a Thin Layer Chromatography technique. Finally, to perform an antimicrobial essay testing some of most common pathogens in human and animal health all provided by the Ricardo Palma University Microbiology Laboratory, Lima, Peru. Results included not only a successful and simplified crude extraction of the carotenoid pigment, but probed its antibacterial activity against some pathogens strains such as V. parahaemolyticus, P. fluorescens and R. equi. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950 10.31381/biotempo.v18i1.3950 |
url |
http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950 |
identifier_str_mv |
10.31381/biotempo.v18i1.3950 |
dc.language.none.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950/4789 |
dc.rights.none.fl_str_mv |
Derechos de autor 2021 Biotempo info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Derechos de autor 2021 Biotempo |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Facultad de Ciencias Biológicas, Universidad Ricardo Palma |
publisher.none.fl_str_mv |
Facultad de Ciencias Biológicas, Universidad Ricardo Palma |
dc.source.none.fl_str_mv |
Biotempo; Vol. 18 Núm. 1 (2021): Biotempo Journal Manuscript accepted, early view 2519-5697 1992-2159 reponame:Revista URP - Biotempo instname:Universidad Ricardo Palma instacron:URP |
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Revista URP - Biotempo |
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Revista URP - Biotempo |
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Universidad Ricardo Palma |
instacron_str |
URP |
institution |
URP |
repository.name.fl_str_mv |
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|
repository.mail.fl_str_mv |
mail@mail.com |
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1701653912505810944 |
score |
13.754011 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).