EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS

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Micrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial proper...

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Detalles Bibliográficos
Autores: Fernández-Sánchez, Harley, Agurto-Sáenz, Tomás, Gonzales Molfino, Hugo Mauricio, Chavieri-Salazar, Andrés Ricardo, Guerra-Santa Cruz, Alcides
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Ricardo Palma
Repositorio:Revista URP - Biotempo
Lenguaje:español
OAI Identifier:oai:oai.revistas.urp.edu.pe:article/3950
Enlace del recurso:http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950
Nivel de acceso:acceso abierto
Materia:Micrococcus luteus
carotenoide
pigmento
antimicrobiano
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spelling EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUSEXTRACCIÓN, CARACTERIZACIÓN Y ACTIVIDAD ANTIMICROBIANA DEL PIGMENTO CAROTENOIDE DE MICROCOCCUS LUTEUSFernández-Sánchez, HarleyAgurto-Sáenz, TomásGonzales Molfino, Hugo MauricioChavieri-Salazar, Andrés RicardoGuerra-Santa Cruz, AlcidesMicrococcus luteuscarotenoidepigmentoantimicrobianoMicrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial properties that may depend on the origin of isolation. Currently, it is important to explore several food grade pigments from natural sources and their potential effects on the food industry, human or veterinary health since they tend to be less harmful. For that reason, the first objective of the study was to extract this carotenoid pigment from M. luteus isolated from the environment with volatile organic solvents. Second, to characterize this pigment using a Thin Layer Chromatography technique. Finally, to perform an antimicrobial essay testing some of most common pathogens in human and animal health all provided by the Ricardo Palma University Microbiology Laboratory, Lima, Peru. Results included not only a successful and simplified crude extraction of the carotenoid pigment, but probed its antibacterial activity against some pathogens strains such as V. parahaemolyticus, P. fluorescens and R. equi.Micrococcus luteus (Schroeter) Cohn 1872 es una de las bacterias que se encuentran en el ambiente, sus colonias presentan una pigmentación amarillenta. Este pigmento es un carotenoide cuyas propiedades varían según el ambiente de origen o aislamiento. En la actualidad, es importante explorar varias fuentes naturales de colorantes de grado alimenticio y sus potenciales efectos en la industria alimentaria, la salud humana o veterinaria. Por ello, el presente trabajo tuvo como objetivo realizar la extracción del pigmento carotenoide de M. luteus con solventes orgánicos y la respectiva caracterización mediante cromatografía en capa fina (TLC) del pigmento carotenoide de M. luteus aislado del Laboratorio de Microbiología de la Universidad Ricardo Palma, Lima, Perú, para, posteriormente, evaluar su actividad antimicrobiana frente a una serie de cepas proporcionadas por este mismo laboratorio. Consecuentemente, se obtuvo no solo una extracción simplificada cruda del pigmento con étil acetato y un factor de retención (R/ f)  de 0.4 para ese pigmento, sino que también se confirmó su actividad antibacterial contra algunas cepas patógenas de importancia humana y veterinaria como lo son V. parahaemolyticus, P. fluorescens y R. equi.Facultad de Ciencias Biológicas, Universidad Ricardo Palma2021-06-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistas.urp.edu.pe/index.php/Biotempo/article/view/395010.31381/biotempo.v18i1.3950Biotempo; Vol. 18 Núm. 1 (2021): Biotempo Journal Manuscript accepted, early view2519-56971992-2159reponame:Revista URP - Biotempoinstname:Universidad Ricardo Palmainstacron:URPspahttp://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950/4789Derechos de autor 2021 Biotempoinfo:eu-repo/semantics/openAccess2021-06-04T16:20:16Zmail@mail.com -
dc.title.none.fl_str_mv EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
EXTRACCIÓN, CARACTERIZACIÓN Y ACTIVIDAD ANTIMICROBIANA DEL PIGMENTO CAROTENOIDE DE MICROCOCCUS LUTEUS
title EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
spellingShingle EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
Fernández-Sánchez, Harley
Micrococcus luteus
carotenoide
pigmento
antimicrobiano
title_short EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
title_full EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
title_fullStr EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
title_full_unstemmed EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
title_sort EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF CAROTENOID PIGMENT FROM MICROCOCCUS LUTEUS
dc.creator.none.fl_str_mv Fernández-Sánchez, Harley
Agurto-Sáenz, Tomás
Gonzales Molfino, Hugo Mauricio
Chavieri-Salazar, Andrés Ricardo
Guerra-Santa Cruz, Alcides
author Fernández-Sánchez, Harley
author_facet Fernández-Sánchez, Harley
Agurto-Sáenz, Tomás
Gonzales Molfino, Hugo Mauricio
Chavieri-Salazar, Andrés Ricardo
Guerra-Santa Cruz, Alcides
author_role author
author2 Agurto-Sáenz, Tomás
Gonzales Molfino, Hugo Mauricio
Chavieri-Salazar, Andrés Ricardo
Guerra-Santa Cruz, Alcides
author2_role author
author
author
author
dc.subject.none.fl_str_mv Micrococcus luteus
carotenoide
pigmento
antimicrobiano
topic Micrococcus luteus
carotenoide
pigmento
antimicrobiano
dc.description.none.fl_txt_mv Micrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial properties that may depend on the origin of isolation. Currently, it is important to explore several food grade pigments from natural sources and their potential effects on the food industry, human or veterinary health since they tend to be less harmful. For that reason, the first objective of the study was to extract this carotenoid pigment from M. luteus isolated from the environment with volatile organic solvents. Second, to characterize this pigment using a Thin Layer Chromatography technique. Finally, to perform an antimicrobial essay testing some of most common pathogens in human and animal health all provided by the Ricardo Palma University Microbiology Laboratory, Lima, Peru. Results included not only a successful and simplified crude extraction of the carotenoid pigment, but probed its antibacterial activity against some pathogens strains such as V. parahaemolyticus, P. fluorescens and R. equi.
Micrococcus luteus (Schroeter) Cohn 1872 es una de las bacterias que se encuentran en el ambiente, sus colonias presentan una pigmentación amarillenta. Este pigmento es un carotenoide cuyas propiedades varían según el ambiente de origen o aislamiento. En la actualidad, es importante explorar varias fuentes naturales de colorantes de grado alimenticio y sus potenciales efectos en la industria alimentaria, la salud humana o veterinaria. Por ello, el presente trabajo tuvo como objetivo realizar la extracción del pigmento carotenoide de M. luteus con solventes orgánicos y la respectiva caracterización mediante cromatografía en capa fina (TLC) del pigmento carotenoide de M. luteus aislado del Laboratorio de Microbiología de la Universidad Ricardo Palma, Lima, Perú, para, posteriormente, evaluar su actividad antimicrobiana frente a una serie de cepas proporcionadas por este mismo laboratorio. Consecuentemente, se obtuvo no solo una extracción simplificada cruda del pigmento con étil acetato y un factor de retención (R/ f)  de 0.4 para ese pigmento, sino que también se confirmó su actividad antibacterial contra algunas cepas patógenas de importancia humana y veterinaria como lo son V. parahaemolyticus, P. fluorescens y R. equi.
description Micrococcus luteus (Schroeter) Cohn 1872 is one of the bacteria found in the environment that presents colonies with a very characteristic yellowish color. Previously, this color was identified as the result of carotenoid production in this species and that it can have a variety of beneficial properties that may depend on the origin of isolation. Currently, it is important to explore several food grade pigments from natural sources and their potential effects on the food industry, human or veterinary health since they tend to be less harmful. For that reason, the first objective of the study was to extract this carotenoid pigment from M. luteus isolated from the environment with volatile organic solvents. Second, to characterize this pigment using a Thin Layer Chromatography technique. Finally, to perform an antimicrobial essay testing some of most common pathogens in human and animal health all provided by the Ricardo Palma University Microbiology Laboratory, Lima, Peru. Results included not only a successful and simplified crude extraction of the carotenoid pigment, but probed its antibacterial activity against some pathogens strains such as V. parahaemolyticus, P. fluorescens and R. equi.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950
10.31381/biotempo.v18i1.3950
url http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950
identifier_str_mv 10.31381/biotempo.v18i1.3950
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.urp.edu.pe/index.php/Biotempo/article/view/3950/4789
dc.rights.none.fl_str_mv Derechos de autor 2021 Biotempo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2021 Biotempo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Facultad de Ciencias Biológicas, Universidad Ricardo Palma
publisher.none.fl_str_mv Facultad de Ciencias Biológicas, Universidad Ricardo Palma
dc.source.none.fl_str_mv Biotempo; Vol. 18 Núm. 1 (2021): Biotempo Journal Manuscript accepted, early view
2519-5697
1992-2159
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instname:Universidad Ricardo Palma
instacron:URP
reponame_str Revista URP - Biotempo
collection Revista URP - Biotempo
instname_str Universidad Ricardo Palma
instacron_str URP
institution URP
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repository.mail.fl_str_mv mail@mail.com
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