Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)

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The aim of this study was to identify and serotype isolate strains from vendors’ hands, chopping boards and vending tables of 50 chicken meat stalls in a market in San Juan de Miraflores district, Lima, Peru. The sampling was carried out with sterile swabs on the indicated surfaces. The Stuard mediu...

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Detalles Bibliográficos
Autores: Morales-Cauti, Siever, Salazar V., Elinor, Ampuero-Riega, Lizette, Navarro O., Armando
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/19042
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19042
Nivel de acceso:acceso abierto
Materia:serotyping
E. coli
poultry meat
serotipificación
carne de ave
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spelling Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)Serotipificación de Escherichia coli aislados a partir de superficies vivas e inertes en un mercado de carne de pollo (Lima, Perú)Morales-Cauti, SieverSalazar V., ElinorAmpuero-Riega, LizetteNavarro O., ArmandoserotypingE. colipoultry meatserotipificaciónE. colicarne de aveThe aim of this study was to identify and serotype isolate strains from vendors’ hands, chopping boards and vending tables of 50 chicken meat stalls in a market in San Juan de Miraflores district, Lima, Peru. The sampling was carried out with sterile swabs on the indicated surfaces. The Stuard medium was used to transport the samples to the laboratory. The enrichment was carried out in trypticase soy broth and the isolation was developed on McConkey agar. Positive lactose colonies compatible with E. coli were identified by standard biochemical tests. Serotyping was performed using the method described by Kauffman to detect somatic (O) and flagellar (H) antigens with specific SERUNAM antisera. Results showed that 42% (63/150) of the samples were identified as E. coli, and 58 viable strains were serotyped, belonging to 40 serotypes, where O6H10 was the most frequent (10.3%, 6/58).  El presente estudio tuvo por objetivo identificar y serotipificar cepas aisladas de manos de vendedores, tabla de picar y mesas de expendio de 50 puestos de venta de carne de pollo de un mercado de abasto del distrito de San Juan de Miraflores, Lima, Perú. La toma de muestra fue realizada con hisopos estériles sobre las superficies indicadas. En el transporte de las muestras se utilizó el medio Stuard, el enriquecimiento se realizó en caldo de tripticasa de soya y el aislamiento se desarrolló en agar McConkey. Las colonias lactosas positivas compatibles con E. coli se identificaron mediante pruebas bioquímicas estándar. La serotipificación se realizó utilizando el método descrito por Kauffman para detectar el antígeno somático (O) y flagelar (H) con antisueros específicos SERUNAM. El 42% (63/150) de las muestras fueron identificadas como E. coli, lográndose serotipificar 58 cepas viables que pertenecen a 40 serotipos, donde el O6H10 fue el de mayor frecuencia (10.3%, 6/58).Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1904210.15381/rivep.v31i4.19042Revista de Investigaciones Veterinarias del Perú; Vol 31 No 4 (2020); e19042Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e190421682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19042/16136Derechos de autor 2020 Siever Morales-Cauti, Elinor Salazar V., Lizette Ampuero-Riega, Armando Navarro O.http://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T18:11:00Zmail@mail.com -
dc.title.none.fl_str_mv Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
Serotipificación de Escherichia coli aislados a partir de superficies vivas e inertes en un mercado de carne de pollo (Lima, Perú)
title Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
spellingShingle Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
Morales-Cauti, Siever
serotyping
E. coli
poultry meat
serotipificación
E. coli
carne de ave
title_short Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
title_full Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
title_fullStr Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
title_full_unstemmed Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
title_sort Serotyping of Escherichia coli isolated from living and inert surfaces in a chicken meat market (Lima, Peru)
dc.creator.none.fl_str_mv Morales-Cauti, Siever
Salazar V., Elinor
Ampuero-Riega, Lizette
Navarro O., Armando
author Morales-Cauti, Siever
author_facet Morales-Cauti, Siever
Salazar V., Elinor
Ampuero-Riega, Lizette
Navarro O., Armando
author_role author
author2 Salazar V., Elinor
Ampuero-Riega, Lizette
Navarro O., Armando
author2_role author
author
author
dc.subject.none.fl_str_mv serotyping
E. coli
poultry meat
serotipificación
E. coli
carne de ave
topic serotyping
E. coli
poultry meat
serotipificación
E. coli
carne de ave
dc.description.none.fl_txt_mv The aim of this study was to identify and serotype isolate strains from vendors’ hands, chopping boards and vending tables of 50 chicken meat stalls in a market in San Juan de Miraflores district, Lima, Peru. The sampling was carried out with sterile swabs on the indicated surfaces. The Stuard medium was used to transport the samples to the laboratory. The enrichment was carried out in trypticase soy broth and the isolation was developed on McConkey agar. Positive lactose colonies compatible with E. coli were identified by standard biochemical tests. Serotyping was performed using the method described by Kauffman to detect somatic (O) and flagellar (H) antigens with specific SERUNAM antisera. Results showed that 42% (63/150) of the samples were identified as E. coli, and 58 viable strains were serotyped, belonging to 40 serotypes, where O6H10 was the most frequent (10.3%, 6/58).  
El presente estudio tuvo por objetivo identificar y serotipificar cepas aisladas de manos de vendedores, tabla de picar y mesas de expendio de 50 puestos de venta de carne de pollo de un mercado de abasto del distrito de San Juan de Miraflores, Lima, Perú. La toma de muestra fue realizada con hisopos estériles sobre las superficies indicadas. En el transporte de las muestras se utilizó el medio Stuard, el enriquecimiento se realizó en caldo de tripticasa de soya y el aislamiento se desarrolló en agar McConkey. Las colonias lactosas positivas compatibles con E. coli se identificaron mediante pruebas bioquímicas estándar. La serotipificación se realizó utilizando el método descrito por Kauffman para detectar el antígeno somático (O) y flagelar (H) con antisueros específicos SERUNAM. El 42% (63/150) de las muestras fueron identificadas como E. coli, lográndose serotipificar 58 cepas viables que pertenecen a 40 serotipos, donde el O6H10 fue el de mayor frecuencia (10.3%, 6/58).
description The aim of this study was to identify and serotype isolate strains from vendors’ hands, chopping boards and vending tables of 50 chicken meat stalls in a market in San Juan de Miraflores district, Lima, Peru. The sampling was carried out with sterile swabs on the indicated surfaces. The Stuard medium was used to transport the samples to the laboratory. The enrichment was carried out in trypticase soy broth and the isolation was developed on McConkey agar. Positive lactose colonies compatible with E. coli were identified by standard biochemical tests. Serotyping was performed using the method described by Kauffman to detect somatic (O) and flagellar (H) antigens with specific SERUNAM antisera. Results showed that 42% (63/150) of the samples were identified as E. coli, and 58 viable strains were serotyped, belonging to 40 serotypes, where O6H10 was the most frequent (10.3%, 6/58).  
publishDate 2020
dc.date.none.fl_str_mv 2020-12-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19042
10.15381/rivep.v31i4.19042
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19042
identifier_str_mv 10.15381/rivep.v31i4.19042
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19042/16136
dc.rights.none.fl_str_mv Derechos de autor 2020 Siever Morales-Cauti, Elinor Salazar V., Lizette Ampuero-Riega, Armando Navarro O.
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Siever Morales-Cauti, Elinor Salazar V., Lizette Ampuero-Riega, Armando Navarro O.
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol 31 No 4 (2020); e19042
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19042
1682-3419
1609-9117
reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
reponame_str Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
collection Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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score 13.905282
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