Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria

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The objective of this research was to compare the antibacterial activity in vitro of the hydroalcoholic extracts of three medicinal plants: Plantago major L. (plantago), Erythroxylum novogranatense var truxillense (trujillo coca) and Camellia sinensis (green tea) by the agar disk diffusion method on...

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Autores: Alvarado Villanueva, Verónica, Moromi Nakata, Hilda
Formato: artículo
Fecha de Publicación:2010
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Odontología Sanmarquina
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/2853
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/2853
Nivel de acceso:acceso abierto
Materia:Medicinal plants
Antibacterial effect
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
Plantas medicinales
efecto antibacteriano
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network_name_str Revista UNMSM - Odontología Sanmarquina
dc.title.none.fl_str_mv Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
Plantas medicinales: Efecto antibacteriano in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense y Camellia sinensis sobre bacterias de importancia estomatológica
title Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
spellingShingle Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
Alvarado Villanueva, Verónica
Medicinal plants
Antibacterial effect
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
Plantas medicinales
efecto antibacteriano
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
title_short Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
title_full Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
title_fullStr Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
title_full_unstemmed Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
title_sort Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteria
dc.creator.none.fl_str_mv Alvarado Villanueva, Verónica
Moromi Nakata, Hilda
author Alvarado Villanueva, Verónica
author_facet Alvarado Villanueva, Verónica
Moromi Nakata, Hilda
author_role author
author2 Moromi Nakata, Hilda
author2_role author
dc.subject.none.fl_str_mv Medicinal plants
Antibacterial effect
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
Plantas medicinales
efecto antibacteriano
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
topic Medicinal plants
Antibacterial effect
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
Plantas medicinales
efecto antibacteriano
Plantago major L
Erythroxylum novograntense var truxillense
Camellia sinensis.
dc.description.none.fl_txt_mv The objective of this research was to compare the antibacterial activity in vitro of the hydroalcoholic extracts of three medicinal plants: Plantago major L. (plantago), Erythroxylum novogranatense var truxillense (trujillo coca) and Camellia sinensis (green tea) by the agar disk diffusion method on five strains of oral bacteria: Streptococcus mutans ATCC 25175, Lactobacillus acidophilus. ATCC 314, Actinomyces viscosus ATCC 15987, Prevotella melaninogenica ATCC 25845 and Fusobacterium nucleatum ATCC 25586. The hydroalcoholic extracts were obtained from total active principles from the dry leaves of each plant, by maceration in alcohol with ethyl alcohol at 70 % and subsequent evaporation of the solvent with the use of a rotary evaporator. Each extract was diluted in ethyl alcohol at 70% in concentrations of 25 ug/ mL and 50 ug/mL. These solutions were compared with Perio Aid® (chlorhexidine 0.012%) as positive control and with ethyl alcohol at 70% as negative control. When performing the sensitivity tests in vitro, the following results were obtained: the three hydroalcoholic extracts in both concentrations showed a higher antibacterial activity than the ethyl alcohol (5.8 mm) and lower than the Perio Aid® (22.0 mm) on the five bacterial strains being studied. The highest activity showed the hydroalcoholic extract of Camellia sinensis at 50 ug/mL, the lowest activity showed Plantago major L. at 25 ug/mL. It is concluded that the three hydroalcoholic extracts in the dissolutions of 25 and 50 μg/mL showed antibacterial activity on Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Prevotella melaninogenica and Fusobacterium nucleatum. The antibacterial effect was increased with the concentration on P. melaninogenica, which was the most sensitive strain, and A. viscosus was the less sensitive.
El objetivo de la investigación fue comparar la actividad antibacteriana in vitro de los extractos hidroalcohólicos de tres plantas medicinales: Plantago major L. (llantén), Erythroxylum novograntense var truxillense (coca trujillo) y Camellia sinensis (té verde) mediante el método de difusión en agar con discos, sobre cinco cepas patrones de bacterias orales: Streptococcus mutans ATCC 25175, Lactobacillus acidophilus. ATCC 314, Actinomyces viscosus ATCC 15987, Prevotella melaninogenicus ATCC 25845 y Fusobacterium nucleatum ATCC 25586. Se obtuvieron extractos hidroalcohólicos de principios activos totales procedentes de las hojas secas de cada una de las plantas, mediante maceración alcohólica con alcohol etílico al 70 % y posterior evaporación del solvente con el empleo del rotavapor. Cada extracto se diluyó en alcohol etílico al 70 % en las concentraciones de 25 μg/mL y 50 μg/mL. Estas soluciones fueron comparadas con PerioAid® (clorhexidina 0,012 %) como control positivo y con alcohol etílico al 70 %, como control negativo. Al realizar las pruebas de sensibilidad in vitro, se obtuvieron los siguientes resultados: los tres extractos hidroalcohólicos en ambas concentraciones presentaron actividad antibacteriana mayor al alcohol etílico (5,8 mm) y menor que el PerioAid® (22,0 mm) sobre las cinco cepas bacterianas en estudio. La mayor actividad presentó el extracto hidroalcohólico de Camellia sinensis a 50 μg/mL, la menor actividad presento Plantago major a 25 μg/mL. Se concluye que los tres extractos hidroalcohólicos en las diluciones de 25 y 50 μg/mL presentaron actividad antibacteriana sobre Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Prevotella melaninogenica y Fusobacterium nucleatum. El efecto antibacteriano aumentó con la concentración en P. melaninogenica, que fue la cepa más sensible y A. viscosus la menos sensible.
description The objective of this research was to compare the antibacterial activity in vitro of the hydroalcoholic extracts of three medicinal plants: Plantago major L. (plantago), Erythroxylum novogranatense var truxillense (trujillo coca) and Camellia sinensis (green tea) by the agar disk diffusion method on five strains of oral bacteria: Streptococcus mutans ATCC 25175, Lactobacillus acidophilus. ATCC 314, Actinomyces viscosus ATCC 15987, Prevotella melaninogenica ATCC 25845 and Fusobacterium nucleatum ATCC 25586. The hydroalcoholic extracts were obtained from total active principles from the dry leaves of each plant, by maceration in alcohol with ethyl alcohol at 70 % and subsequent evaporation of the solvent with the use of a rotary evaporator. Each extract was diluted in ethyl alcohol at 70% in concentrations of 25 ug/ mL and 50 ug/mL. These solutions were compared with Perio Aid® (chlorhexidine 0.012%) as positive control and with ethyl alcohol at 70% as negative control. When performing the sensitivity tests in vitro, the following results were obtained: the three hydroalcoholic extracts in both concentrations showed a higher antibacterial activity than the ethyl alcohol (5.8 mm) and lower than the Perio Aid® (22.0 mm) on the five bacterial strains being studied. The highest activity showed the hydroalcoholic extract of Camellia sinensis at 50 ug/mL, the lowest activity showed Plantago major L. at 25 ug/mL. It is concluded that the three hydroalcoholic extracts in the dissolutions of 25 and 50 μg/mL showed antibacterial activity on Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Prevotella melaninogenica and Fusobacterium nucleatum. The antibacterial effect was increased with the concentration on P. melaninogenica, which was the most sensitive strain, and A. viscosus was the less sensitive.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/2853
10.15381/os.v13i2.2853
url https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/2853
identifier_str_mv 10.15381/os.v13i2.2853
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/2853/2435
dc.rights.none.fl_str_mv Derechos de autor 2010 Verónica Alvarado Villanueva, Hilda Moromi Nakata
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2010 Verónica Alvarado Villanueva, Hilda Moromi Nakata
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Odontología
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Odontología
dc.source.none.fl_str_mv Odontología Sanmarquina; Vol 13 No 2 (2010); 21-25
Odontología Sanmarquina; Vol. 13 Núm. 2 (2010); 21-25
1609-8617
1560-9111
reponame:Revista UNMSM - Odontología Sanmarquina
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
reponame_str Revista UNMSM - Odontología Sanmarquina
collection Revista UNMSM - Odontología Sanmarquina
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
repository.name.fl_str_mv -
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spelling Medicinal plants: Antibacterial effect in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense and Camellia sinensis on stomatologic importance bacteriaPlantas medicinales: Efecto antibacteriano in vitro de Plantago major L, Erythroxylum novogranatense, Plowman var truxillense y Camellia sinensis sobre bacterias de importancia estomatológicaAlvarado Villanueva, VerónicaMoromi Nakata, HildaMedicinal plantsAntibacterial effectPlantago major LErythroxylum novograntense var truxillenseCamellia sinensis.Plantas medicinalesefecto antibacterianoPlantago major LErythroxylum novograntense var truxillenseCamellia sinensis.The objective of this research was to compare the antibacterial activity in vitro of the hydroalcoholic extracts of three medicinal plants: Plantago major L. (plantago), Erythroxylum novogranatense var truxillense (trujillo coca) and Camellia sinensis (green tea) by the agar disk diffusion method on five strains of oral bacteria: Streptococcus mutans ATCC 25175, Lactobacillus acidophilus. ATCC 314, Actinomyces viscosus ATCC 15987, Prevotella melaninogenica ATCC 25845 and Fusobacterium nucleatum ATCC 25586. The hydroalcoholic extracts were obtained from total active principles from the dry leaves of each plant, by maceration in alcohol with ethyl alcohol at 70 % and subsequent evaporation of the solvent with the use of a rotary evaporator. Each extract was diluted in ethyl alcohol at 70% in concentrations of 25 ug/ mL and 50 ug/mL. These solutions were compared with Perio Aid® (chlorhexidine 0.012%) as positive control and with ethyl alcohol at 70% as negative control. When performing the sensitivity tests in vitro, the following results were obtained: the three hydroalcoholic extracts in both concentrations showed a higher antibacterial activity than the ethyl alcohol (5.8 mm) and lower than the Perio Aid® (22.0 mm) on the five bacterial strains being studied. The highest activity showed the hydroalcoholic extract of Camellia sinensis at 50 ug/mL, the lowest activity showed Plantago major L. at 25 ug/mL. It is concluded that the three hydroalcoholic extracts in the dissolutions of 25 and 50 μg/mL showed antibacterial activity on Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Prevotella melaninogenica and Fusobacterium nucleatum. The antibacterial effect was increased with the concentration on P. melaninogenica, which was the most sensitive strain, and A. viscosus was the less sensitive.El objetivo de la investigación fue comparar la actividad antibacteriana in vitro de los extractos hidroalcohólicos de tres plantas medicinales: Plantago major L. (llantén), Erythroxylum novograntense var truxillense (coca trujillo) y Camellia sinensis (té verde) mediante el método de difusión en agar con discos, sobre cinco cepas patrones de bacterias orales: Streptococcus mutans ATCC 25175, Lactobacillus acidophilus. ATCC 314, Actinomyces viscosus ATCC 15987, Prevotella melaninogenicus ATCC 25845 y Fusobacterium nucleatum ATCC 25586. Se obtuvieron extractos hidroalcohólicos de principios activos totales procedentes de las hojas secas de cada una de las plantas, mediante maceración alcohólica con alcohol etílico al 70 % y posterior evaporación del solvente con el empleo del rotavapor. Cada extracto se diluyó en alcohol etílico al 70 % en las concentraciones de 25 μg/mL y 50 μg/mL. Estas soluciones fueron comparadas con PerioAid® (clorhexidina 0,012 %) como control positivo y con alcohol etílico al 70 %, como control negativo. Al realizar las pruebas de sensibilidad in vitro, se obtuvieron los siguientes resultados: los tres extractos hidroalcohólicos en ambas concentraciones presentaron actividad antibacteriana mayor al alcohol etílico (5,8 mm) y menor que el PerioAid® (22,0 mm) sobre las cinco cepas bacterianas en estudio. La mayor actividad presentó el extracto hidroalcohólico de Camellia sinensis a 50 μg/mL, la menor actividad presento Plantago major a 25 μg/mL. Se concluye que los tres extractos hidroalcohólicos en las diluciones de 25 y 50 μg/mL presentaron actividad antibacteriana sobre Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus, Prevotella melaninogenica y Fusobacterium nucleatum. El efecto antibacteriano aumentó con la concentración en P. melaninogenica, que fue la cepa más sensible y A. viscosus la menos sensible.Universidad Nacional Mayor de San Marcos, Facultad de Odontología2010-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/285310.15381/os.v13i2.2853Odontología Sanmarquina; Vol 13 No 2 (2010); 21-25Odontología Sanmarquina; Vol. 13 Núm. 2 (2010); 21-251609-86171560-9111reponame:Revista UNMSM - Odontología Sanmarquinainstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/2853/2435Derechos de autor 2010 Verónica Alvarado Villanueva, Hilda Moromi Nakatahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T17:15:28Zmail@mail.com -
score 13.887938
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