Objectives: To determine metformin influence in in vitro fertilization (IVF) and intracytoplasmatic sperm injection (ICSI) success rates in women with polycystic ovaries (PCO). Design: Retrospective study. Setting: Fertility and Gynecology Southern Center, Cusco, Peru. Participants: Women with polycystic ovaries  associated infertility. Methods: We compared IVF/ICSI success rates in 7 women with diagnosis of PCO who  used metformin and 9 women with PCO who did not use metformin. Main outcomes measures: Pregnancy. Results:  In PCO women using metformin implantation rate was 35%, biochemical pregnancy rate 71,4%, and clinical pregnancy rate 57,1%. In PCO women not using metformin, success rates were respectively 0%, 11,1%, and 0%. Conclusions: Metformin use improved IVF/ICSI success rates in PCO women.

Oocytes in vitro maturation (IVM) usually used in patients with polycystic ovaries and polycystic ovary syndrome has been applied for the treatment of infertility in a woman with secondary amenorrea and history of anorexia nervosa. After repeated unsuccessful attempts of ovarian stimulation for conventional techniques of assisted reproduction, IVM treatment was begun by stimulating the ovaries with rFSH during the second, third and fourth day of the cycle and aspiring antral follicles on the eighth day. There were four cumulus-oocyte complexes that were cultivated in the maturation media for 36 hours in presence of FSH and hCG. Mature metaphase II oocytes were fertilized by intracytoplamatic sperm injection (ICSI) and embryos were frozen by vitrification on the second day. A month later, the endometrium was prepared with estradiol valerate. Thawed embryos were cultivated for 24 additiona...

Introduction: Intrauterine insemination is part of the strategies for initial management in infertile couples. However, limited research has taken place in our country regarding its effectiveness in the pursuit of pregnancy, and it is therefore important to define patients, indications and conditions associated with higher rates of clinical pregnancy. Objective: To identify factors associated with clinical pregnancy in intrauterine insemination. Methods: Retrospective study in 633 infertile couples at the Centro de Fertilidad y Ginecologia del Sur, Cusco, Peru. 1 053 cycles of intrauterine insemination were performed in both spontaneous and stimulated ovulation, with double or single insemination performed before or after ovulation, using a semen sample from the spouse, processed either by swim-up or by density gradients. Results: Clinical pregnancy rate was 8.2% per insemination cycle a...

Objective: To determine the success rates of in vitro fertilization (IVF) in an assisted reproductive technology program at 3 330 meters above sea level. Design: Descriptive, retrospective study. Setting: Centro de Fertilidad y Ginecologia del Sur, Cusco, Peru, city at 3 330 meters (10 989 feet) above sea level. Participants: Couples with infertility diagnosis and indication of IVF. Interventions: We performed IVF and embryo transfer in 27 couples. Main outcome measures: Implantation, biochemical pregnancy, and clinical pregnancy rates. Results: Implantation rate was 14,3%, biochemical pregnancy rate 37,0% and clinical pregnancy rate 22,2%. Conclusions: IVF cycles at 3 330 meters above sea level seem to achieve implantation and clinical pregnancy rates similar to national and international reports.

Oocytes in vitro maturation (IVM) usually used in patients with polycystic ovaries and polycystic ovary syndrome has been applied for the treatment of infertility in a woman with secondary amenorrea and history of anorexia nervosa. After repeated unsuccessful attempts of ovarian stimulation for conventional techniques of assisted reproduction, IVM treatment was begun by stimulating the ovaries with rFSH during the second, third and fourth day of the cycle and aspiring antral follicles on the eighth day. There were four cumulus-oocyte complexes that were cultivated in the maturation media for 36 hours in presence of FSH and hCG. Mature metaphase II oocytes were fertilized by intracytoplamatic sperm injection (ICSI) and embryos were frozen by vitrification on the second day. A month later, the endometrium was prepared with estradiol valerate. Thawed embryos were cultivated for 24 additiona...

Objectives: To determine metformin influence in in vitro fertilization (IVF) and intracytoplasmatic sperm injection (ICSI) success rates in women with polycystic ovaries (PCO). Design: Retrospective study. Setting: Fertility and Gynecology Southern Center, Cusco, Peru. Participants: Women with polycystic ovaries  associated infertility. Methods: We compared IVF/ICSI success rates in 7 women with diagnosis of PCO who  used metformin and 9 women with PCO who did not use metformin. Main outcomes measures: Pregnancy. Results:  In PCO women using metformin implantation rate was 35%, biochemical pregnancy rate 71,4%, and clinical pregnancy rate 57,1%. In PCO women not using metformin, success rates were respectively 0%, 11,1%, and 0%. Conclusions: Metformin use improved IVF/ICSI success rates in PCO women.

Objective: To determine the success rates of in vitro fertilization (IVF) in an assisted reproductive technology program at 3 330 meters above sea level. Design: Descriptive, retrospective study. Setting: Centro de Fertilidad y Ginecologia del Sur, Cusco, Peru, city at 3 330 meters (10 989 feet) above sea level. Participants: Couples with infertility diagnosis and indication of IVF. Interventions: We performed IVF and embryo transfer in 27 couples. Main outcome measures: Implantation, biochemical pregnancy, and clinical pregnancy rates. Results: Implantation rate was 14,3%, biochemical pregnancy rate 37,0% and clinical pregnancy rate 22,2%. Conclusions: IVF cycles at 3 330 meters above sea level seem to achieve implantation and clinical pregnancy rates similar to national and international reports.

Introduction: Infertility is currently a more frequent diagnosis, at sea level and at high altitudes, and development of high complexity assisted reproduction techniques in the Andes of Peru is essential. Objective: To define the results of the application of these techniques in an institution at 3 330 meters above sea level. Design: Descriptive and retrospective study. Setting: Centro de Fertilidad y Ginecología del Sur, Cusco, Peru. Patients: 576 patients with infertility attended between years 2005-2015. Methods: Records of the procedures performed were reviewed and rates of clinical pregnancy according to age and days of culture were established for both autologous oocytes and ovodonation. Statistical significance of differences found was analyzed by Fisher exact test. Main outcome measure: Rate of clinical pregnancy. Results: Rates of clinical pregnancy were: 30.4% in autologous oo...