La sangre, el tejido de la vida, ha sido designada de esta manera porque refleja el balance de nuestro organismo en un equilibrio perfecto. Debido a sus múltiples funciones (transporte de oxígeno y nutrientes a las células, recolección de los productos de desecho, transporte de hormonas, intervención en el equilibrio de ácidos, sales, bases y agua en el interior de las células y en la regulación de la temperatura del cuerpo, defensa contra agentes externos y mecanismos de coagulación) representa una increíble fuente de información del estado biológico y químico del cuerpo y hasta la actualidad su análisis es una de las herramientas más importantes en la medicina.

La sangre, el tejido de la vida, ha sido designada de esta manera porque refleja el balance de nuestro organismo en un equilibrio perfecto. Debido a sus múltiples funciones (transporte de oxígeno y nutrientes a las células, recolección de los productos de desecho, transporte de hormonas, intervención en el equilibrio de ácidos, sales, bases y agua en el interior de las células y en la regulación de la temperatura del cuerpo, defensa contra agentes externos y mecanismos de coagulación) representa una increíble fuente de información del estado biológico y químico del cuerpo y hasta la actualidad su análisis es una de las herramientas más importantes en la medicina.

Universidad Nacional Agraria La Molina. Facultad de Ciencias. Departamento Académico de Biología

Using the primordial germ cells transplant technique, we could be able preserve and multiply pluripotent cells in the receptor for a long period of time. In this work, We aim to evaluate intraluminal colonization of a cellular gonocyte suspension from 14.5 dpc fetus. Cellular suspension with PGC's were isolated from fetus male mice by two enzymatic digestion steps, and cellular suspensions were transplanted into the rete testis of the receptor animals that were previously injected with Busulfan to decrease their own spermatogenesis. In this research the intraluminal colonization was identified in 13.27%, demonstrating that transplantation of a cellular suspension from gonocytes of fetus of 14.5 dpc containing PGCs can colonize the seminiferous tubules and support the spermatogenesis.

Using the primordial germ cells transplant technique, we could be able preserve and multiply pluripotent cells in the receptor for a long period of time. In this work, We aim to evaluate intraluminal colonization of a cellular gonocyte suspension from 14.5 dpc fetus. Cellular suspension with PGC's were isolated from fetus male mice by two enzymatic digestion steps, and cellular suspensions were transplanted into the rete testis of the receptor animals that were previously injected with Busulfan to decrease their own spermatogenesis. In this research the intraluminal colonization was identified in 13.27%, demonstrating that transplantation of a cellular suspension from gonocytes of fetus of 14.5 dpc containing PGCs can colonize the seminiferous tubules and support the spermatogenesis.

Objective: to establish the ratios of the copy number variations and regions of homozygosity through chromosomal microarray analysis (CMA) in children with neurodevelopmental disorders: development delay (DD), intellectual disability (ID), and/or autistic spectrum disorder (ASD), malformative syndrome (MS) and idiopathic short stature (ISS). Materials and methods: we evaluated 367 Peruvian children diagnosed clinically with ID, DD, ASD, ISS and MS to whom performed chromosomal microarray analysis in peripheral blood (750K CGH + SNP), between the years 2016-2018. Results: patients' age fluctuated between 4.8 months and 18 years old, with an average of 5.6 years old. The most frequent diagnoses were development delay (48%) and intellectual disability (30%). Abnormal results (pathogenic variants, likely pathogenic variants, uniparental disomies and loss of heterozygosity> 2.5%) were repo...

Objective: to establish the ratios of the copy number variations and regions of homozygosity through chromosomal microarray analysis (CMA) in children with neurodevelopmental disorders: development delay (DD), intellectual disability (ID), and/or autistic spectrum disorder (ASD), malformative syndrome (MS) and idiopathic short stature (ISS). Materials and methods: we evaluated 367 Peruvian children diagnosed clinically with ID, DD, ASD, ISS and MS to whom performed chromosomal microarray analysis in peripheral blood (750K CGH + SNP), between the years 2016-2018. Results: patients' age fluctuated between 4.8 months and 18 years old, with an average of 5.6 years old. The most frequent diagnoses were development delay (48%) and intellectual disability (30%). Abnormal results (pathogenic variants, likely pathogenic variants, uniparental disomies and loss of heterozygosity> 2.5%) were repo...