We develop some bioinformatics procedures to predict Conserved Ortholog Set (COS) sequences from Ipomoea batatas, and evaluate their usefulness for molecular markers and diversity studies of this species. We predict orthology relationship between Ipomoea batatas ESTs sequences and Arabidopsis thaliana COS sequences, according to Best Bidirectional Hits (BBHs) criteria, realizing similarity comparison using BLAST X and TBLASTN programs. We obtained a set of 204 putative COS sequences, 16 of them belonged to storage roots. Functional annotation of sweet potato predicted sequences COS was realized using BLASTX, INTERPROSCAN and PSI-BLAST programs. We evaluate possible polymorphisms in COS candidate sequences, finding SNPs in eight sequences and tandem repeats in one of them.

This work uses bioinformatics tools and free online access to genomic information in order to identify and characterize proteins related to oxidative stress defense of Mycobacterium tuberculosis, in order to disco ver new appropriate pharmacological targets against this pathogen. Sequences of amino acids of proteins expressed with that function, products of the expression of the genes katG, sodA, sodC, ahpC, ahpD and mgtC were compared with the genome of Mycobacterium tuberculosis H37Rv through the NCBI server National for Center Biotechnology Information), determining their possible redundancy in the genome. Parallely, information was obtained above of their evolutive conserved sequences by global multiply alignment using MAXHOM program, as well models of their threedimensionalstructures by comparative modeling method through the Swiss-model server, or using «recognition of folding» m...

En el presente trabajo se describe una serie de procedimientos bioinformáticos para la predicción de un grupo secuencias ortólogas conservadas (COS) de Ipomoea batatas, así como la evaluación de su potencial utilidad para la generación de marcadores moleculares y estudios de diversidad en esta especie. Con ese propósito usando los programas BLAST X y TBLASTN se realizó una comparación reciproca por similaridad entre secuencias ESTs procedentes de librerías de cDNAs de Ipomoea batatas, propias o disponibles de modo público en la base de datos GenBank, con secuencias COS de Arabidopsis thaliana. La anotación funcional de las secuencias COS predichas en Ipomoea batatas se realizo usando los programas BLASTX, INTERPROSCAN y PSI-BLAST. Se obtuvieron en total 204 secuencias COS candidatos de Ipomoea batatas, siendo 16 secuencias provenientes de una librería generada a partir de ra...

This work uses bioinformatics tools and free online access to genomic information in order to identify and characterize proteins related to oxidative stress defense of Mycobacterium tuberculosis, in order to disco ver new appropriate pharmacological targets against this pathogen. Sequences of amino acids of proteins expressed with that function, products of the expression of the genes katG, sodA, sodC, ahpC, ahpD and mgtC were compared with the genome of Mycobacterium tuberculosis H37Rv through the NCBI server National for Center Biotechnology Information), determining their possible redundancy in the genome. Parallely, information was obtained above of their evolutive conserved sequences by global multiply alignment using MAXHOM program, as well models of their threedimensionalstructures by comparative modeling method through the Swiss-model server, or using «recognition of folding» m...

Phospholipases D are enzymes present in all eucariotic organisms, it was first discovered in plants and in recent years, increasing attention has been paid to its roles in signal transduction in mammals. Human isoforms of this enzyme D1 and D2 were analyzed using bioinformatic tools, predicting their chemical properties responsible of their different levels of action. Besides, using local multiply alignment programs like MACAW with amino acid sequences from different organisms, three conserved blocks of sequences in all organisms were detected. Between Plant sequences and not with bacterial, high similarity was detected. When these conserved blocks of sequence was visualized in determined experimentally 3D structure of Phospolipase D from Streptomyces Sp , an structural zone having near these blocks of sequence, was observed. This zone is postulated like an important structural feature o...

Family I lipases are industrially recognized for their catalytic activities of esterification, interesterification and transesterification. In this study, Marinobacter sp. LB lipase isolated from Salinas de Pilluana, San Martín was characterized by in silico analysis. For this purpose, lip gene was amplified by conventional Polymerase Chain Reaction (PCR) and nucleotide sequence was analyzed in silico. The tertiary structure was elucidated using the 1EX9 lipase from Pseudomonas aeruginosa PAO1 as a template and molecular docking was executed with three substrates. The lip gene had 927 bp and mature protein, 284 amino acids. The lipase had a molecular weight of 29.99 kDa and pI of 8.89. Also typicall catalytic triad residues of family I lipases (Ser78, Asp229 and His251) were identified. In addition, eleven peripheral α-helixs and seven internal β-sheets were found. Binding pocket and ...

Las lipasas de la familia I son reconocidas a nivel industrial por sus actividades catalíticas de esterificación, interesterificación y transesterificación. En esta investigación se caracterizó por análisis in silico a la lipasa de Marinobacter sp. LB aislado de las Salinas de Pilluana, San Martín. Con tal finalidad, se amplificó el gen lip mediante la reacción en cadena de la polimerasa (PCR) de punto final y la secuencia nucleotídica fue analizada in silico. Se elucidó la estructura terciaria empleando como molde a la lipasa 1EX9 de Pseudomonas aeruginosa PAO1 y se ejecutó el acoplamiento molecular con tres sustratos. El gen lip presentó 927 pb y la proteína madura, 284 aminoácidos. La lipasa posee un peso molecular de 29.99 kDa y un pI de 8.89. Asimismo, se identificaron residuos Ser78, Asp229 e His251, típicos de la triada catalítica de una lipasa de la familia I. A...