Se determinó el efecto citotóxico de la Tartrazina en el índice mitótico de células meristemáticas de Allium cepa expuesta a diferentes tiempos y concentraciones. Para ello, bulbos enraizados de A. cepa se expusieron a 0 mg/mL, 0.1 mg/mL, 1mg/mL y 5mg/mL de Tartrazina durante 8 y 24 horas en un diseño jerárquico. Posteriormente, las raicillas fueron aisladas, fijadas y coloreadas para realizar el preparado citológico y su posterior lectura al microscopio. Los resultados mostraron una disminución del índice mitótico (IM) en todos los tratamientos ensayados con respecto al control y testigos, siendo el tratamiento  de 5 mg/mL-8 horas el que generó un menor valor del IM (3.3 %). Asimismo, se observó la disminución del índice de fases en todos los tratamientos con respecto al control. Se concluye que la Tartrazina disminuye el Índice mitótico y de fases de células meriste...

Se determinó el efecto citotóxico de la Tartrazina en el índice mitótico de células meristemáticas de Allium cepa expuesta a diferentes tiempos y concentraciones. Para ello, bulbos enraizados de A. cepa se expusieron a 0 mg/mL, 0.1 mg/mL, 1mg/mL y 5mg/mL de Tartrazina durante 8 y 24 horas en un diseño jerárquico. Posteriormente, las raicillas fueron aisladas, fijadas y coloreadas para realizar el preparado citológico y su posterior lectura al microscopio. Los resultados mostraron una disminución del índice mitótico (IM) en todos los tratamientos ensayados con respecto al control y testigos, siendo el tratamiento  de 5 mg/mL-8 horas el que generó un menor valor del IM (3.3 %). Asimismo, se observó la disminución del índice de fases en todos los tratamientos con respecto al control. Se concluye que la Tartrazina disminuye el Índice mitótico y de fases de células meriste...

The aim of this research work was evaluate toxic activity of ethanolic extracts from Argemone subfusiformis “Holy thistle” & Tagetes patula “French marigold” leaves against Aedes aegypti fourth instar larvae and pupae were evaluated. Bioassay and extract processing were performed in Entomology Laboratory of National University of Trujillo, Perú, from April to December, 2007, outlined by World Health Organization (2005) standard protocol. Larvae and pupae mortality rates reached using A. subfusiformis extract were 100% with concentrations of 76,8 mg/L. and 153,6 mg/L. at 12 hours of exposure, while pupae mortality was to a concentration of 153,6 mg/L. at 24 hours of exposure. By using T. patula extract mortality rates reached were 92% and 77% on larvae and pupae, respectively with a concentration of 153,6 mg/L. at 48 hours of exposure. A. subfusiformis extracts showed LC50 an...

En el presente trabajo se evaluó el efecto tóxico de los extractos etanólicos foliares de Argemone subfusiformis “cardo santo” y Tagetes patula “marigold” sobre larvas IV y pupas de Aedes aegypti. El procesamiento de los extractos y los bioensayos se realizaron en el Laboratorio de Entomología de la Universidad Nacional de Trujillo, Perú, de abril a diciembre de 2007, basados en los lineamientos metodológicos de la World Health Organization (2005). En larvas, se registró 100% de mortalidad con 76,8 y 153,6 mg/L del extracto de A. subfusiformis a las 12 horas de exposición, mientras que en pupas el mismo porcentaje de mortalidad se alcanzó con 153,6 mg/L a las 24 horas. De otro lado, el 92% y 77% de mortalidad en larvas y pupas respectivamente se registró con el extracto de T. patula al emplear 153,6 mg/L del extracto a las 48 horas. En A. subfusiformis las concentracion...

Vitis vinifera «grape» is one edible fruit vine of importance in agriculture activities, besides to have medical properties related to bioactive natural products knowing as anthocyanins, that, they exhibit antioxidative, anticancerigen and cardiotonic activities. In this paper, a system of suspension cell cultures from Vitis vinifera «vid» var. red globe was established to determine the effect of the concentration of sucrose on anthocyanins yield. Different concentrations of sucrose (0 mM, 58 mM, 132 mM and 175 mM) into Basal culture Media (BM) supplemented with separated concentrations of naftalnacetic acid and kinetine as Inductor Media (IM), were applied. Anthocyanins yield were increased in according to increment of sucrose concentration (132mM) in the cell culture. Significant differences among the treatments were founded.

Vitis vinifera «grape» is one edible fruit vine of importance in agriculture activities, besides to have medical properties related to bioactive natural products knowing as anthocyanins, that, they exhibit antioxidative, anticancerigen and cardiotonic activities. In this paper, a system of suspension cell cultures from Vitis vinifera «vid» var. red globe was established to determine the effect of the concentration of sucrose on anthocyanins yield. Different concentrations of sucrose (0 mM, 58 mM, 132 mM and 175 mM) into Basal culture Media (BM) supplemented with separated concentrations of naftalnacetic acid and kinetine as Inductor Media (IM), were applied. Anthocyanins yield were increased in according to increment of sucrose concentration (132mM) in the cell culture. Significant differences among the treatments were founded.

The aim of the present study was to evaluate the larvicidal toxicity of aqueous suspensions from ethanol extracts of Annona muricata L. «custard apple» seeds, flowers, leaves, stem bark and root bark on fourth instar larvae of Aedes aegypti L. (Diptera: Culicidae) as well as to determinate susceptibility level . The highest larvicidal toxic effect was to the seeds suspension with 100% mortality at 0,5 mg/mL from 24 hours of exposure, and flowers suspension at 10 mg/mL from 48 hours of exposure and leaves suspension at 100 mg/mL from 36 hours of exposure. In seed suspension, lethal concentrations 50% (LC50) and 90% (LC90 ) values were 0,02 mg/mL and 0,11 mg/mL, in flowers suspensions were 3,33 mg/mL and 12,16 mg/mL, in leaf suspensions were 8,25 y 26,87 mg/mL and stem bark suspensions were 97,23 mg/mL, respectively. Log-dosage/probit lines indicated homogeneous responses towards the fiv...

The aim of the present study was to evaluate the larvicidal toxicity of aqueous suspensions from ethanol extracts of Annona muricata L. «custard apple» seeds, flowers, leaves, stem bark and root bark on fourth instar larvae of Aedes aegypti L. (Diptera: Culicidae) as well as to determinate susceptibility level . The highest larvicidal toxic effect was to the seeds suspension with 100% mortality at 0,5 mg/mL from 24 hours of exposure, and flowers suspension at 10 mg/mL from 48 hours of exposure and leaves suspension at 100 mg/mL from 36 hours of exposure. In seed suspension, lethal concentrations 50% (LC50) and 90% (LC90 ) values were 0,02 mg/mL and 0,11 mg/mL, in flowers suspensions were 3,33 mg/mL and 12,16 mg/mL, in leaf suspensions were 8,25 y 26,87 mg/mL and stem bark suspensions were 97,23 mg/mL, respectively. Log-dosage/probit lines indicated homogeneous responses towards the fiv...