El protozoo Sarcocystis aucheniae es el agente causal de sarcocistosis en llamas y alpacas; la enfermedad en su fase aguda es generalmente asintomática, es decir que no muestra signos específicos, aunque el parásito está circulando en sangre. El presente estudio tuvo como objetivo establecer la sensibilidad analítica y especificidad de la técnica molecular PCR semi-anidada para el diagnóstico de S. aucheniae en sangre de alpacas de la provincia de Huancavelica. Para determinar la sensibilidad analítica de la PCR semi-anidada se utilizó ADN genómico extraído de macroquistes de S. aucheniae con el que se realizó la prueba a concentraciones muy bajas (1 ng, 0,1 ng, 0,01 ng, 1 pg, y 0.01 pg). La especificidad de la PCR semi-anidada se determinó al comparar la prueba molecular en sangre con el método de necropsia; para ello se colectó muestras de sangre de alpacas de Huancaveli...

Background: Colibacillosis caused by Escherichia coli causes significant economic losses in the livestock sector worldwide and is one of the calves’ leading causes of diarrea Aim: This study aimed to identify the most frequent E. coli molecularly pathotypes in calves with diarrhea in six provinces of the Cajamarca region in the northern highlands of Peru. Methods: Twenty-eight herds of dairy cattle under a semi-intensive rearing system were evaluated; 95 samples were isolated from calves with diarrhea up to the first month of life, 62 males and 33 females, during the rainy season. Results: The presence of virulence genes of E. coli strains was more prevalent in males; the astA (89.47%), st (83.15%), and f5 (57.89%) genes were more expressed, and the lt (17.89%) and stx2 (1.05%) genes were less expressed. The eae gene (21.05%) was more present in females. Conclusion: When E. coli strain...

Background: Colibacillosis caused by Escherichia coli causes significant economic losses in the livestock sector worldwide and is one of the calves’ leading causes of diarrea Aim: This study aimed to identify the most frequent E. coli molecularly pathotypes in calves with diarrhea in six provinces of the Cajamarca region in the northern highlands of Peru. Methods: Twenty-eight herds of dairy cattle under a semi-intensive rearing system were evaluated; 95 samples were isolated from calves with diarrhea up to the first month of life, 62 males and 33 females, during the rainy season. Results: The presence of virulence genes of E. coli strains was more prevalent in males; the astA (89.47%), st (83.15%), and f5 (57.89%) genes were more expressed, and the lt (17.89%) and stx2 (1.05%) genes were less expressed. The eae gene (21.05%) was more present in females. Conclusion: When E. coli strain...