The objective of the investigation was to determine the biological characteristics of the skin's collagen in Oreochromis niloticus “tilapia”, referred to performance, quantification of total proteins, electrophoresis SDS-PAGE, thermal stability, morphology of the surface structure by optical microscopy and the amount of collagen type I obtained by the methods of aqueous soluble collagen (ASC) and by pepsin soluble collagen (PSC). The biological material, tilapia, was obtained at the Experimental Center of Genetics of the National University of Trujillo. The weights of the individuals were recorded and the skin of the fish was extracted and after registering the skin weight of each individual, the collagen was extracted. By the ASC, a pretreatment was carried out with and without sodium hydroxide 0.1M/24h, followed by the application of butanol to 10 %and extraction in acetic acid 0.5...

El objetivo de la investigación fue determinar las características biológicas del colágeno extraído de la piel de Oreochromis niloticus, tilapia, referido al rendimiento, cuantificación de proteínas totales, electroforesis SDS-PAGE, estabilidad térmica, morfología de la estructura superficial por microscopía óptica y la cantidad de colágeno tipo I obtenido por los métodos colágeno soluble acuoso (ASC) y por, colágeno soluble con pepsina (PSC). El material biológico, tilapia, fue obtenido en el Centro Experimental de Genética de la Universidad Nacional de Trujillo. Se registraron los pesos de los individuos y se extrajo la piel de los peces y luego de registrar el peso de piel de cada individuo se procedió con la extracción de colágeno.

This research was funded by CONCYTEC-FONDECYT, process E041-01 (grant number 123-2018-FONDECYT-BM-IADT/AV), CNPq-Brazil, grant number 305250/2019-1, and CANON MINERO-UNT (P.I.I.01-2013).

The authors thank CONCYTEC-FONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-BM-IADT/AV), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Process 305250/2019-1).

Authors are very thankful to CONCYTECFONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-BM-IADT/AV), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Process 305250/2019-1, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) Finance Code 001 and Programa Nacional de Pós-Doutorado (PNPD).