The aim of this study was to develop a pharmacokinetic model of florfenicol in plasma of tilapia (Oreochromis niloticus) subjected to different rearing temperatures to estimate the plasma concentration of the protocols indicated in the package inserts of florfenicol products authorized in Brazil. For this, a pharmacokinetic model was created from previously published data on the plasma concentration of florfenicol in Nile tilapia subjected to different temperatures. The construction of the pharmacokinetic model was carried out with the Lixoft® Monolix 2020R1 program, based on the plasma pharmacokinetic data of florfenicol found in the literature. Plasma concentrations of florfenicol were estimated from treatments with doses of 10, 15 and 20 mg/kg administered every 24 hours for 10 days, at temperatures of 18, 21, 26 and 30 °C, using the Lixoft Simulx 2020 program®. The absorption lat...