Dr. Juana María Coha was a professor at the Universidad Nacional Mayor de San Marcos, recognized for her inner energy and intense personality, which could be perceived in her courses and in particular in conversations with her students. With a great desire to promote research and solve problems in the country, it became the guide of many promotions of microbiologists. Juana María Coha Gonzáles, was born on June 24, 1926 in the city of Huacho and died on July 22, 2017. These lines are written by her students and disciples that we continue what she forged in her lines of research.

Dr. Juana María Coha was a professor at the Universidad Nacional Mayor de San Marcos, recognized for her inner energy and intense personality, which could be perceived in her courses and in particular in conversations with her students. With a great desire to promote research and solve problems in the country, it became the guide of many promotions of microbiologists. Juana María Coha Gonzáles, was born on June 24, 1926 in the city of Huacho and died on July 22, 2017. These lines are written by her students and disciples that we continue what she forged in her lines of research.

En el presente trabajo se ensayó una técnica de degradación biológica del explosivo 2,4,6 trinitrotolueno (TNT) encondiciones in vitro. Se realizó un screening de cepas bacterianas degradadoras de TNT a partir de 18 cepasprovenientes de suelo contaminado con derivados de hidrocarburo y de un sistema de tratamiento de relaves mineros.Se seleccionaron dos cepas: UP1 y 3T412C, identificadas por análisis de la secuencia parcial del gen rRNA 16S como Serratia marcescens UP1 y Klebsiella oxytoca 3T412C con los que se formó un consorcio evaluándose la degradación de 60 mg/1 de TNT en medio Mínimo Modificado con succinato como única fuente de carbono a temperatura ambiente (28 ± 2°C) en  agitación constante a 150 rpm por 72 horas. La concentración de TNT del medio de cultivo fue cuantificada por Cromatografía Líquida de Alta Eficacia (HPLC). El porcentaj...