Genetic association analysis between snp rs914458 of protein tyrosine phosphatase, non- receptor type 1(ptpn1) gene and Type 2 Diabetes in Peruvian Population

Objective: To determine the association of microsatellite markers D4S2912, D4S230 and D4S3001 with diabetes type 2 (DM2) in the Peruvian population. Material and Methods: We analyzed the microsatellite markers D4S2912, D4S230 and D4S3001 in 99 diabetics and 120 healthy individuals to D4S2912, D4S230 and 129 to 133 for D0453001 as controls from Lima. We processed the 0NA extracted from blood intravenously, amplified by the technique of polymerase chain reaction (PCR) for the three markers in the region 4p15.1. Finally, we analyzed the polymorphisms of the markers and determined the genetic homogeneity of the population through proof of Hardy-Weinberg (HW), and continued in the study of association of 0M2 in patients versus controls. Results: In D4S2912 and D4S3001 there was absence of an allele in the control group when compared with the diabetic population. When performing association an...

La diabetes tipo 2 es una enfermedad compleja que tiene un componente genético. Se ha comprobado que calpaí­na 10 (CAPN10) localizado en 2q37.3, constituye un gen de susceptibilidad para esta enfermedad. La asociación alélica y haplotípica con diabetes, observada en poblacio­nes amerindias (méxico-americana, pima, surui y maya), y algunas europeas (finlandesa y alemana), confirmarían es­tos resultados. El origen ancestral amerindio nos hace su­poner que CAPN10 constituiría un gen de susceptibilidad en población peruana nativa y mestiza.

The objective of this investigation is to test the hypothesis of the possible association of one or more microsatellites located on 425-4933 with NSCLP. In this study a sample of 51 extended pedigrees were analyzed. From these pedigrees a sample of 51 caseparents trios was obtained. A novel genetic analysis was carried out using the Extended Transmission Disequilibrium Test (ETDT). Also a case control study was carried out with the 51 probands of the case-parents trios plus a sample of 94 controls of the Chilean population to test if differences were observed resulting from the methods used for the association studies. Microsatellites D4S1570, D4S1615, FGA, UCP1 and D4S1597 located in 4q were analyzed. The polymerase chain reaction (PCR) was used for analysis of DNA. Forward primers were marked with fluorescent-dye-label. Electrophoresis and analyses were carried out in an ABI PRISM 377 ...

Alchomea castaneifoUa (WiU) Jussieu es una planta medicinal perteneciente a la familia Euforbiáceae, conocida con numerosos nombres populares y múltiples usos en la Medicina Tradicional. En el presente trabajo, se evaluó la acción citotóxica del extracto metanólico al 10% p/v, en modelos del CYTED, siguiendo la técnica de De la Torre y la evaluación de la acción embriotóxica por la técnica de Gustafson y Estus. Observamos un retardo en el desarrollo del ciclo celular de AUium cepa y un retardo del desarrollo embrionario del erizo de mar, a nivel de blástula y gástrula, asícomo un aumento significativo de la mortalidad.En el análisis fitoquímico, de acuerdo a la Metodología de Ciulei, se pudo observar la presencia de: alcaloides, esteroides, triterpenos, azúcares reductores, saponinas, fenoles, taninos, quinonas, flavonoides, antocianidinas y principios amargos.

Objective: To investigate the changes in the plasma-circulating adiponectin concentration in 23 premenopausal obese women living in Lima after a body mass reduction as a result of an aerobic physical activity program and a low-calorie diet. Materials and methods: An analytical and quasi-experimental study was conducted in a group of obese women. In addition, another 24 women were considered as control or reference group for comparing their adiponectin concentration and other biochemical markers. The quantification of adiponectin was carried out using the ELISA test. Fasting blood glucose concentration, cholesterol, high-density lipoprotein and triglycerides levels were quantified by routine clinical analysis. Results: Before beginning the program, the control group showed high adiponectin levels (median, 8.54 µg/mL; range, 6.14 µg/ mL to 13.49 µg/mL) compared to the obesity group (med...

Objective: To analyze the association that PTPN1 gene single nucleotide polymorphisms (SNPs) rs941798 and rs914458 have with type 2 diabetes in Peruvian families from Lima. Materials and methods: Twenty-three (23) families consisting of three members each were recruited at the Hospital Nacional Arzobispo Loayza. Peripheral blood samples were collected to obtain the DNA, and then the allele and genotype frequencies of the SNPs. SNP genotyping was performed using the sequencing method. The family-based analysis of the association between SNPs and type 2 diabetes was conducted using the family-based association test (FBAT) program. Results: Three (3) possible genotypes were observed for each SNP, i.e. rs941798 (A>G) and rs914458 (G>C). In one of the assessed models, the family-based association tests showed at the allele level that allele A of SNP rs941798 is associated with type 2 d...