The present research was conducted in order to determine the parameters of production and conservation of adult bovine rennet. Dry abomasum was chunked and proceeded to the extraction of the enzyme with hydrochloric acid 3 mM, pH 2,5. Was activated enzyme solution and filtered to yield the liquid rennet with 1:607,59 force (± 1,58) Soxhlet Units. Liquid rennet was filled in low density polyethylene milky white bottles and stored for one month at 4°C, -7°C and -198°C, to later evaluation. The strength of liquid rennet in storage at 4°C did not yield good results, because loss of the 38,47% of force. Stored liquid rennet to temperature of -7°C, generated a power loss of 30,94%, being the basal strength of 1:419,58 (± 1,47) in U.S. (Units Soxhlet). Storage of liquid rennet to -198°C (liquid nitrogen), was appropriate to preserve with power loss.

The present research was conducted in order to determine the parameters of production and conservation of adult bovine rennet. Dry abomasum was chunked and proceeded to the extraction of the enzyme with hydrochloric acid 3 mM, pH 2,5. Was activated enzyme solution and filtered to yield the liquid rennet with 1:607,59 force (± 1,58) Soxhlet Units. Liquid rennet was filled in low density polyethylene milky white bottles and stored for one month at 4°C, -7°C and -198°C, to later evaluation. The strength of liquid rennet in storage at 4°C did not yield good results, because loss of the 38,47% of force. Stored liquid rennet to temperature of -7°C, generated a power loss of 30,94%, being the basal strength of 1:419,58 (± 1,47) in U.S. (Units Soxhlet). Storage of liquid rennet to -198°C (liquid nitrogen), was appropriate to preserve with power loss.

The aim of this study is to molecularly characterize bacteria with probiotic potential isolated from feces of human neonates. Sixty stool samples from neonates (0-3 days) were evaluated and enriched in Man Rogosa and Sharp (MRS) broth at 37 ° C / 24h. It was selected and subjected to in vitro tests with bile salts, resistance to low pH and antimicrobial activity against Escherichia coli ATCC25922, E. coli ATCC35218, Salmonella enterica and Listeria inocua by agar diffusion assay. The molecular identification was made with PCR-BOX amplifications and the sequencing of the 16S rRNA gene. A total of 48 strains were isolated and all showed resistance to pH 3 and 0.3% bile salts; 3 strains showed antimicrobial activity against E. coli ATCC25922, 1 strain against E. coli ATCC35218, 5 strains against L. innocuous and all against S. enterica. Of the 48 strains, two BOX-PCR profiles belonging to ...

The aim of this study is to molecularly characterize bacteria with probiotic potential isolated from feces of human neonates. Sixty stool samples from neonates (0-3 days) were evaluated and enriched in Man Rogosa and Sharp (MRS) broth at 37 ° C / 24h. It was selected and subjected to in vitro tests with bile salts, resistance to low pH and antimicrobial activity against Escherichia coli ATCC25922, E. coli ATCC35218, Salmonella enterica and Listeria inocua by agar diffusion assay. The molecular identification was made with PCR-BOX amplifications and the sequencing of the 16S rRNA gene. A total of 48 strains were isolated and all showed resistance to pH 3 and 0.3% bile salts; 3 strains showed antimicrobial activity against E. coli ATCC25922, 1 strain against E. coli ATCC35218, 5 strains against L. innocuous and all against S. enterica. Of the 48 strains, two BOX-PCR profiles belonging to ...

El objetivo fue evaluar la inclusión de harina quinua Altiplano cocida (HQA), fibra soluble del endospermo de tara (FST) e insoluble de hojas de agave (FIA) sobre propiedades sensoriales de galletas y su respuesta fisiológica en ratas. Se realizó la evaluación sensorial con participación de consumidores mediante prueba hedónica con escala no estructurada. Para la evaluación biológica, se utilizó 20 ratas Holtzman que recibieron durante 30 días las dietas conteniendo galletas con: T1 = harina de trigo, T2 = 15% HQA, T3 = 2,85% FST + 2,85% FIA, T4 = 15% HQA + 2,85% FST + 2,85% FIA. Se realizó la determinación de valor biológico aparente (VBA) e indicadores somáticos (índice de masa corporal, índice de Lee, circunferencia torácica y abdominal). Los datos fueron analizados bajo Diseño Completamente Randomizado y para comparación de medias se utilizó la prueba de Tukey med...

El objetivo fue evaluar la inclusión de harina quinua Altiplano cocida (HQA), fibra soluble del endospermo de tara (FST) e insoluble de hojas de agave (FIA) sobre propiedades sensoriales de galletas y su respuesta fisiológica en ratas. Se realizó la evaluación sensorial con participación de consumidores mediante prueba hedónica con escala no estructurada. Para la evaluación biológica, se utilizó 20 ratas Holtzman que recibieron durante 30 días las dietas conteniendo galletas con: T1 = harina de trigo, T2 = 15% HQA, T3 = 2,85% FST + 2,85% FIA, T4 = 15% HQA + 2,85% FST + 2,85% FIA. Se realizó la determinación de valor biológico aparente (VBA) e indicadores somáticos (índice de masa corporal, índice de Lee, circunferencia torácica y abdominal). Los datos fueron analizados bajo Diseño Completamente Randomizado y para comparación de medias se utilizó la prueba de Tukey med...