Microbe eukaryotes like fungi and protista are common in the drainage of mines. Few information is known on microbial diversity of acid drainages of Peruvian mines. In this work we isolated and characterized the 1M9 yeast. We utilized LSU D1/D2 of the 26S rRNA gene sequence phylogenetic analyses to characterize the diversity the yeast 1MA9 isolated. The strain 1MA9 was closely related to the Pichia guilliermondii. The yeast showed high resistance to Mn2+ (>400 mM), intermediate to ions Zn2+ and Co2+ (400—600 mM), but sensible to ions Cu2+. This work provides the first data on yeasts from an aquatic acid environment of Peruvian mines.

The Oomycete Phytophthora infestans (Mont.) de Bary, the causal agent of the disease known as late blight, is primarily responsible for the decreased in production performance and potato crops worldwide. The integration of the complete Rgenes sequences in the potato genome using Agro-transformation appears an alternative to be considered in the fight against this pathogen. The Rpi-blb2 gene (Rgene) from the wild species Solanum bulbocastanumDunal shows a broad resistance to isolates ofP. infestans,making it an important candidate for plant breeding studies. This paper reports the integration of the Rpi-blb2gene into potato var. Désirée genome by Agrobacterium tumefaciens- mediated transformation system, the molecular characterization of 29 events transformed and whole plant infection with isolate POX67 of P. infestansfrom Peru. Désirée events [Rpi-blb2] 4 and Désirée [Rpi-blb2] 30,...

Las aguas ácidas de minas tienen una considerable diversidad de microorganismos eucariontes, entre ellos hongos y protistas; en particular, poco se conoce sobre la diversidad de levaduras en drenajes ácidos de minas peruanas. En el presente estudio se aisló y caracterizó la levadura 1MA9, mediante la amplificación y secuenciación del LSU D1/D2 del gen rRNA 26S. La cepa 1MA9 se identificó molecularmente como Pichia guillermondi, ésta presentó una resistencia alta a Mn2+ (>400 mM), intermedia a iones Zn2+ y Co2+ (400—600 mM), pero fue sensible a iones Cu2+. Este trabajo representa el primer reporte de levaduras en ambientes acuáticos ácidos proveniente de minas peruanas.

The Oomycete Phytophthora infestans (Mont.) de Bary, the causal agent of the disease known as late blight, is primarily responsible for the decreased in production performance and potato crops worldwide. The integration of the complete Rgenes sequences in the potato genome using Agro-transformation appears an alternative to be considered in the fight against this pathogen. The Rpi-blb2 gene (Rgene) from the wild species Solanum bulbocastanumDunal shows a broad resistance to isolates ofP. infestans,making it an important candidate for plant breeding studies. This paper reports the integration of the Rpi-blb2gene into potato var. Désirée genome by Agrobacterium tumefaciens- mediated transformation system, the molecular characterization of 29 events transformed and whole plant infection with isolate POX67 of P. infestansfrom Peru. Désirée events [Rpi-blb2] 4 and Désirée [Rpi-blb2] 30,...

One of the most efficient options for the control of late blight disease in potato (Solanum tuberosum) is the development of resistant varieties to Phytophthora infestans mediated by the direct transfer of resistance (R) genes through genetic engineering. In the present work, we used Solanum bulbocastanum RB gene to confers broad spectrum resistance to P. infestans races. To that end, Agrobacterium - mediated genetic transformation was used to transform a susceptible potato variety, Desiree, with the binary vector pCIP68 harboring the RB gene. As a result, 19 transformed plants containing the RB gene were obtained. kanamycin resistance test and polymerase chain reaction (PCR) assays confirmed the integration of the T-DNA in the potato genome. The 19 transformed plants, also called transgenic events were subjected to infection under biosafety greenhouse conditions. Phytophthora infestans ...

One of the most efficient options for the control of late blight disease in potato (Solanum tuberosum) is the development of resistant varieties to Phytophthora infestans mediated by the direct transfer of resistance (R) genes through genetic engineering. In the present work, we used Solanum bulbocastanum RB gene to confers broad spectrum resistance to P. infestans races. To that end, Agrobacterium - mediated genetic transformation was used to transform a susceptible potato variety, Desiree, with the binary vector pCIP68 harboring the RB gene. As a result, 19 transformed plants containing the RB gene were obtained. kanamycin resistance test and polymerase chain reaction (PCR) assays confirmed the integration of the T-DNA in the potato genome. The 19 transformed plants, also called transgenic events were subjected to infection under biosafety greenhouse conditions. Phytophthora infestans ...