Microbe eukaryotes like fungi and protista are common in the drainage of mines. Few information is known on microbial diversity of acid drainages of Peruvian mines. In this work we isolated and characterized the 1M9 yeast. We utilized LSU D1/D2 of the 26S rRNA gene sequence phylogenetic analyses to characterize the diversity the yeast 1MA9 isolated. The strain 1MA9 was closely related to the Pichia guilliermondii. The yeast showed high resistance to Mn2+ (>400 mM), intermediate to ions Zn2+ and Co2+ (400—600 mM), but sensible to ions Cu2+. This work provides the first data on yeasts from an aquatic acid environment of Peruvian mines.

Las aguas ácidas de minas tienen una considerable diversidad de microorganismos eucariontes, entre ellos hongos y protistas; en particular, poco se conoce sobre la diversidad de levaduras en drenajes ácidos de minas peruanas. En el presente estudio se aisló y caracterizó la levadura 1MA9, mediante la amplificación y secuenciación del LSU D1/D2 del gen rRNA 26S. La cepa 1MA9 se identificó molecularmente como Pichia guillermondi, ésta presentó una resistencia alta a Mn2+ (>400 mM), intermedia a iones Zn2+ y Co2+ (400—600 mM), pero fue sensible a iones Cu2+. Este trabajo representa el primer reporte de levaduras en ambientes acuáticos ácidos proveniente de minas peruanas.

Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this species has been reported only once, whereby there is no conclusive role of the plasmids in the species. Herein, two novel plasmids of A. ferrivorans PQ33 were molecularly characterized and compared at a genomic scale. The genomes of two plasmids (12 kbp and 10 kbp) from A. ferrivorans PQ33 (NZ_LVZL01000000) were sequenced and annotated. The plasmids, named pAfPQ33-1 (NZ_CP021414.1) and pAfPQ33-2 (NZ_CP021415.1), presented 9 CDS and 13 CDS, respectively. In silico analysis showed proteins involved in conjugation (TraD, MobA, Eep and XerD), toxin-antitoxin systems (HicA and HicB), replication (RepA and DNA binding protein), transcription regul...

Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this species has been reported only once, whereby there is no conclusive role of the plasmids in the species. Herein, two novel plasmids of A. ferrivorans PQ33 were molecularly characterized and compared at a genomic scale. The genomes of two plasmids (12 kbp and 10 kbp) from A. ferrivorans PQ33 (NZ_LVZL01000000) were sequenced and annotated. The plasmids, named pAfPQ33-1 (NZ_CP021414.1) and pAfPQ33-2 (NZ_CP021415.1), presented 9 CDS and 13 CDS, respectively. In silico analysis showed proteins involved in conjugation (TraD, MobA, Eep and XerD), toxin-antitoxin systems (HicA and HicB), replication (RepA and DNA binding protein), transcription regul...