Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this species has been reported only once, whereby there is no conclusive role of the plasmids in the species. Herein, two novel plasmids of A. ferrivorans PQ33 were molecularly characterized and compared at a genomic scale. The genomes of two plasmids (12 kbp and 10 kbp) from A. ferrivorans PQ33 (NZ_LVZL01000000) were sequenced and annotated. The plasmids, named pAfPQ33-1 (NZ_CP021414.1) and pAfPQ33-2 (NZ_CP021415.1), presented 9 CDS and 13 CDS, respectively. In silico analysis showed proteins involved in conjugation (TraD, MobA, Eep and XerD), toxin-antitoxin systems (HicA and HicB), replication (RepA and DNA binding protein), transcription regul...

Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this species has been reported only once, whereby there is no conclusive role of the plasmids in the species. Herein, two novel plasmids of A. ferrivorans PQ33 were molecularly characterized and compared at a genomic scale. The genomes of two plasmids (12 kbp and 10 kbp) from A. ferrivorans PQ33 (NZ_LVZL01000000) were sequenced and annotated. The plasmids, named pAfPQ33-1 (NZ_CP021414.1) and pAfPQ33-2 (NZ_CP021415.1), presented 9 CDS and 13 CDS, respectively. In silico analysis showed proteins involved in conjugation (TraD, MobA, Eep and XerD), toxin-antitoxin systems (HicA and HicB), replication (RepA and DNA binding protein), transcription regul...

El presente trabajo consiste en el diseño de una nave industrial con puente grúa mediante la aplicación de la norma ANSI/AISC 360-10 Specification for Structural Steel Buildings, tomado de referencia por el Reglamento Nacional de Edificaciones del Perú, Norma E090 Estructuras Metálicas toma en cuenta esta norma varios capítulos para la realización del diseño de estructuras metálicas, es por ello se toma en cuenta esta norma americana. El análisis estructural se realiza mediante el empleo del software Nuevo Metal 3D de Cype Ingenieros, el cual en su versión 2014 incorpora las normas peruanas de diseño sísmico E030 Diseño Sismo resistente y la norma peruana E060 Concreto Armado. El diseño se realiza teniendo en cuenta las cargas de diseño especificadas en la norma peruana E020 Cargas, para la zona de Puno. Se realiza un análisis dinámico modal espectral el cual influye en...