The objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control). The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; ...

El objetivo del presente estudio fue valorar la prueba del azul brillante de cresilo (BCB) como método indirecto para seleccionar ovocitos competentes para la producción in vitro de embriones (PIV). Los complejos cúmulos-ovocitos (COC) fueron obtenidos de dos vaquillas criollas sometidas a dos tratamientos: T1 = COC recuperados por OPU (ovum pick-up) previa estimulación con FSH-LH; T2 = COC recuperados sin previa estimulación de la donante (testigo). Las dos vaquillas fueron alternadas en los dos tratamientos y se hicieron cinco repeticiones. Los COC recuperados fueron clasificados en tipos A, B, C y D. Luego se aplicó la prueba del BCB a cada uno de los tipos de COC para determinar si son BCB+ o BCB-. T1 permitió recuperar 5.2 más COC que T2 (p<0.05). Al aplicar la prueba del BCB se determinó que todos los ovocitos tipo A de T1 y T2 fueron BCB+; es decir, terminaron su creci...