The objective of the present study was to evaluate the bright blue cresyl (BCB) test as an indirect method to select competent oocytes for the in vitro production of embryos (IVP). The cumulus-oocyte complexes (COC) were obtained from two Creole heifers subjected to two treatments: T1 = COC recovered by OPU (ovum pick-up) previous stimulation with FSH-LH; T2 = COC recovered from non-stimulated animals (control). The two heifers were alternated in the two treatments and five repetitions were done. Recovered COCs were classified into types A, B, C and D. Then the BCB test was applied to each of the COC types to determine if they were BCB+ or BCB-. T1 allowed to recover 5.2 more COC than T2 (p<0.05). When applying the BCB test, it was determined that all type A oocytes of T1 and T2 were BCB+; that is, they finished their growth and were ready to start the process of in vitro maturation; ...

El objetivo del presente estudio fue valorar la prueba del azul brillante de cresilo (BCB) como método indirecto para seleccionar ovocitos competentes para la producción in vitro de embriones (PIV). Los complejos cúmulos-ovocitos (COC) fueron obtenidos de dos vaquillas criollas sometidas a dos tratamientos: T1 = COC recuperados por OPU (ovum pick-up) previa estimulación con FSH-LH; T2 = COC recuperados sin previa estimulación de la donante (testigo). Las dos vaquillas fueron alternadas en los dos tratamientos y se hicieron cinco repeticiones. Los COC recuperados fueron clasificados en tipos A, B, C y D. Luego se aplicó la prueba del BCB a cada uno de los tipos de COC para determinar si son BCB+ o BCB-. T1 permitió recuperar 5.2 más COC que T2 (p<0.05). Al aplicar la prueba del BCB se determinó que todos los ovocitos tipo A de T1 y T2 fueron BCB+; es decir, terminaron su creci...

The aim of this research was to describe the morphometry of the testicles and sperm of guinea pigs (Cavia porcellus) of two genetic groups, native and improved, from southern Ecuador. Twenty-two native and 20 improved guinea pigs of the Peru line were used. The animals were reared in elevated cages and fed with forage and concentrate, until reaching the slaughter age of 97 to 112 days. After slaughtering, the testicles and epididymis were removed, and the epididymis was separated of the testis. Measurements of the testicles were done, and sperm was collected from the epididymis by the retrograde flow washing technique. Semen smears were prepared for sperm measurements. The data were processed by GLM analysis of variance and the LSMeans procedure was used to compare the means (SAS v. 9.0). The morphometric parameters of the testes were different (p<0.05) between subpopulations. Opposit...

The aim of this research was to describe the morphometry of the testicles and sperm of guinea pigs (Cavia porcellus) of two genetic groups, native and improved, from southern Ecuador. Twenty-two native and 20 improved guinea pigs of the Peru line were used. The animals were reared in elevated cages and fed with forage and concentrate, until reaching the slaughter age of 97 to 112 days. After slaughtering, the testicles and epididymis were removed, and the epididymis was separated of the testis. Measurements of the testicles were done, and sperm was collected from the epididymis by the retrograde flow washing technique. Semen smears were prepared for sperm measurements. The data were processed by GLM analysis of variance and the LSMeans procedure was used to compare the means (SAS v. 9.0). The morphometric parameters of the testes were different (p<0.05) between subpopulations. Opposit...