The study aims to evaluate the contaminant level of Ochratoxin A (OTA) in Coffea arabica L. "coffee", roasted, purified by immunoaffinity columns and quantified by High Efficiency Liquid Chromatography (HPLC). OTA is a nephrotoxic-carcinogenic mycotoxin capable of causing adverse effects on human and animal health. It is found in many processed and unprocessed foods, including coffee, which is a food with high consumption. Roasted coffee samples were collected in the coffee zone of Chanchamayo, department of Junin-Peru. The separation and purification of the Ochratoxin A was performed by NeoColumn immunoaffinity columns, subsequently quantification was performed by HPLC with fluorescence detector, at an excitation wavelength of 333 nm and emission wavelength of 460 nm. Finally, the test of percent of recovery were carried out at the contaminant level of 5 ppb. The results obtained in roa...

Evalúa el nivel contaminante de OTA en Coffea arabica L. de tipo tostado y verde. La colecta de las muestras de café se realizó en la zona cafetalera de Chanchamayo, Departamento de Junín. El proceso de purificación se llevó a cabo mediante columnas de inmunoafinidad (IAC) y la cuantificación por Cromatografía Líquida de Alta Eficiencia (HPLC), con detector de fluorescencia. Asimismo, se realizaron pruebas de recuperación a los niveles contaminantes de 5 μg/kg y 10 μg/kg, para café tostado y verde, respectivamente. El porcentaje de recuperación al nivel 5 μg/kg fue de 76,2% para el café tostado y al nivel de 10 μg/kg fue de 74,94 % para el café verde. Las muestras de café tostado presentaron concentraciones de 0,216 y 0,444 ppb de OTA, mientras que, de las dos muestras de café verde, una muestra presentó un valor de 0.057 ppb y la otra no superó el límite de detecc...

There are several investigations on the use of food waste to remove contaminants by adsorption. However, a simple route, without chemical activation reagents, is needed for the development of adsorbents. The aim of this study was to develop an adsorbent from plantain peel, using a simple procedure, and to evaluate its capacity to remove phosphate from aqueous solutions at phosphate concentrations similar to those of water sources at risk of eutrophication (0.30 mg/L). The simple pyrolysis method was used in an electric muffle, without chemical activation, using plantain peel as precursor. The variables evaluated were pyrolysis temperature and solution pH. The specific surface area BET, zero loading point of the developed treatments, was determined. Phosphate adsorption was studied in a batch experiment in the presence of calcium ions in solution. Phosphate adsorption was favorable at all...