The lack of precise methods for estimating forest biomass results in both economic losses and incorrect decisions in the management of forest plantations. In response to this issue, this study evaluated the effectiveness of using the DJI Zenmuse L1 LiDAR, mounted on a DJI Matrice 300 RTK UAV, to provide three-dimensional measurements of canopy structure and estimate the aboveground biomass of Eucalyptus globulus. Various LiDAR metrics were employed alongside field measurements to calibrate predictive models using multiple regression and machine learning algorithms. The results at the individual tree level show that RF is the most accurate model, with a coefficient of determination (R²) of 0.76 in the training set and 0.66 in the test set, outperforming Elastic Net (R² of 0.58 and 0.57, respectively). At the plot level, a multiple regression model achieved an R² of 0.647, highlighting ...