1
artículo
Publicado 2020
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The aim of this study was to develop a preliminary physical map of single nucleotide polymorphisms (SNPs) in alpaca using an alpaca/hamster radiation hybrid panel and a bovine high- density SNP genotyping microarray (BovineHD BeadChip-Illumina). The methodology included genotyping 92 alpaca/hamster hybrid cell clones, and four control samples (male alpaca, female alpaca, hamster and 1:10 DNAmixture) with the microarray. After genotyping the alpaca and hamster DNA control samples, only bovine SNPs with a call frequency of 1 were retained. The SNPs identified in the alpaca DNA samples were then filtered to remove those also found in the hamster. From the remaining alpaca SNPs, to decrease the probability of false positives, only those with a call frequency from 0.2 to 0.8 in the 92 hybrid clone samples were retained for the final analysis. The remaining alpaca specific SNPs were tabulated ...
2
artículo
Publicado 2013
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The aim of the present study was to determine the ovulation rate using GnRH (buserelin) and seminal plasma of alpaca as ovulation inductors in alpacas and llamas. For this, 93 alpacas and 92 llamas were distributed in two groups: a group treated with buserelin (42 μg) IM, and a group treated with 1 ml of seminal plasma IM (50/50% seminal plasma of alpaca and PBS + antibiotics). The ovulation rate was 78.7 and 88.9% in alpacas and 80.6 and 70.0% in llamas using buserelin and seminal plasma respectively; and without statistical differences due to type of ovulation inductor, ovary carrying the preovulatory follicle or species.
3
artículo
Publicado 2013
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Se trabajó con 93 alpacas y 92 llamas a fin de determinar el porcentaje de ovulación utilizando GnRH (buserelina) y plasma seminal de alpaca como inductor de ovulación en alpacas y llamas. Los animales se distribuyeron en dos grupos, un grupo tratado con 42 μg de buserelina intramuscular y otro grupo tratado con 1 ml de plasma seminal intramuscular (50/50% de plasma seminal y PBS + antibióticos). La tasa de ovulación fue de 78.7 y 88.9% en alpacas y 80.6 y 70.0 en llamas con buserelina y plasma seminal, respectivamente, no encontrándose diferencias estadísticas entre tipo de inductor, ovario con presencia de folículo preovulatorio o especie.
4
artículo
Publicado 2017
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El objetivo del presente estudio fue identificar los polimorfismos de nucleótido simple (PNSs) usando un panel celular híbrido irradiado alpaca/hámster y una micromatriz de alta densidad del bovino. El análisis bioinformático se realizó en la Universidad Nacional Agraria La Molina. La metodología consistió en la genotipificación del panel celular y cuatro muestras controles utilizando una micromatriz de alta densidad para bovinos (BovineHD BeadChip-Illumina). El panel celular estuvo compuesto por 92 clones celulares híbridos irradiados alpaca/hámster con una retención igual o mayor al 40 % del genoma de alpaca. Los cuatro controles contuvieron ADN genómico de alpaca huacaya macho, alpaca huacaya hembra, la línea celular de hámster A23 y una mezcla de alpaca macho y hámster en una proporción de 1:10. Los análisis de datos fueron ejecutados con los programas GenomeStudio,...
5
artículo
Publicado 2010
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It has been studied the influence of the concentration and the pH of solution of tara gum in the coagulation of colloidal system of Fe(OH)3 with positive colloidal particles. The experimental data show that the coagulation is better for 0,1 g/75 ml solution of tara gum and the optimum pH is 5. The kinetic of coagulation was fast and finished after 5 minute.
6
artículo
Publicado 2010
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It has been studied the influence of the concentration and the pH of solution of tara gum in the coagulation of colloidal system of Fe(OH)3 with positive colloidal particles. The experimental data show that the coagulation is better for 0,1 g/75 ml solution of tara gum and the optimum pH is 5. The kinetic of coagulation was fast and finished after 5 minute.
7
artículo
Publicado 2000
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Percentage of Drug abuse in Peruvian population is being determined only with inquiries and no toxiccological analysis is done. For the present study, 55 National High Schools from Lima, Perú, were selected considering those with high student population. The students age fluctuated between 15 and 19 years old, and 13 301 samples were analysed. The analysis results indicated that 15.9% of students had consumed cocaine; 9.9% marijuana; 0.7% benzodiazepine; 0.3% barbiturates and no one had consumed amphetamines. At the same time of sample collection, an inquiry, was performed results but the did not agree with the ones obtained from toxicological analysis. So, we concluded, that inquiries do not demonstrate the real percentage of drug abuse, since they are not answered with responsability by fue students, may be because of fear or shame to their drug-dependence situation.
8
artículo
Publicado 2000
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Percentage of Drug abuse in Peruvian population is being determined only with inquiries and no toxiccological analysis is done. For the present study, 55 National High Schools from Lima, Perú, were selected considering those with high student population. The students age fluctuated between 15 and 19 years old, and 13 301 samples were analysed. The analysis results indicated that 15.9% of students had consumed cocaine; 9.9% marijuana; 0.7% benzodiazepine; 0.3% barbiturates and no one had consumed amphetamines. At the same time of sample collection, an inquiry, was performed results but the did not agree with the ones obtained from toxicological analysis. So, we concluded, that inquiries do not demonstrate the real percentage of drug abuse, since they are not answered with responsability by fue students, may be because of fear or shame to their drug-dependence situation.
