The present research aims to carry out the analysis of the electrical behavior of the glucose oxidase protein (GOx) and thus be able to establish the parameters to be considered in an impedance equipment to be able to perform hemoglobin readings. From the modification of reagent strips Accu-Chek it was possible to obtain the corresponding GOx readings in 8 samples, which supported the adjustment of the optimal frequency range to be able to perform bioelectric impedance analysis in biological material. The results of the study helped to have a clearer vision to lay the foundations for a future team that aims to detect anemia due to the relationship of GOx with Hemoglobin (HbA1c).

In the midst of an investigation to perform bioelectrical impedance analysis (BIA), we were faced with the need to search for optimally adjusted electrodes to perform reading in small biological samples. The best option to carry out these readings is the well-known gold microelectrodes; however, these are very expensive for our research purposes. For this reason, we found an alternative using Accu-Chek Performa test strips as reading microelectrodes due to their low cost and ease of disposal. This article contains an in-depth detail of the components of the Accu-Chek Performa test strip and the process that was used so that they could be suitable for taking measurements on biological material. In addition, a measurement scheme is shown in conclusion to the operation of the test strip as a microelectrode and the possible problems to consider if it is to be used for future research.

Busca desarrollar un dispositivo capaz de analizar tejido sanguíneo con un método mucho menos invasivo, rápido y altamente confiable haciendo uso de una muestra sanguinea de 0.6 uL. Su principio de funcionamiento se basa en la espectroscopia de impedancia eléctrica (EIE) sobre una tira reactiva dopada con una proteína dimérica que reacciona con la Hemoglobina (Hb1Ac). El presente proyecto fue llevado a cabo dentro del laboratorio de Instrumentación Física y Aplicaciones de la UNMSM (INFISA). Como resultado de este trabajo se pudo observar una diferencia notoria entre los espectros de impedancia correspondientes al diagrama de Nyquist en diferentes concentraciones de hemoglobina en sangre, con lo cual se pudo cumplir el objetivo de poder realizar un equipo capaz de analizar tejido sanguíneo por EIE. Este hallazgo ha sido el producto de una investigación financiada por el Vicerre...