Physalis peruviana L. "aguaymanto" is a Solanaceae highly prized in countries such as Peru, Colombia and Ecuador due to its high nutritional value and numerous medicinal properties. Its cultivation has high variability because it is propagated by seeds, which makes it necessary to maintain its homogeneous morphological and productive characteristics. An alternative is in vitro micropropagation to subsequently achieve its technification and industrialization. Therefore, the objective of this work was to induce shoots and roots in hypocotyls and cotyledons of P. peruviana L. using auxins and cytokinins. Nine combinations were made between the concentrations of 2,4-D (0.25, 0.5 and 1.0 mg / l) and BAP (0.1, 0.25 and 1.0 mg / l), in which 20-day-old explants were introduced. The basal medium was that of Murashige & Skoog supplemented with 3% sucrose, phytagel (0.5%) and pH 6. After 70 da...

Physalis peruviana L. "aguaymanto" is a Solanaceae highly prized in countries such as Peru, Colombia and Ecuador due to its high nutritional value and numerous medicinal properties. Its cultivation has high variability because it is propagated by seeds, which makes it necessary to maintain its homogeneous morphological and productive characteristics. An alternative is in vitro micropropagation to subsequently achieve its technification and industrialization. Therefore, the objective of this work was to induce shoots and roots in hypocotyls and cotyledons of P. peruviana L. using auxins and cytokinins. Nine combinations were made between the concentrations of 2,4-D (0.25, 0.5 and 1.0 mg / l) and BAP (0.1, 0.25 and 1.0 mg / l), in which 20-day-old explants were introduced. The basal medium was that of Murashige & Skoog supplemented with 3% sucrose, phytagel (0.5%) and pH 6. After 70 da...

Physalis peruviana L. "aguaymanto" is a Solanaceae highly prized in countries such as Peru, Colombia and Ecuador due to its high nutritional value and numerous medicinal properties. Its cultivation has high variability because it is propagated by seeds, which makes it necessary to maintain its homogeneous morphological and productive characteristics. An alternative is in vitro micropropagation to subsequently achieve its technification and industrialization. Therefore, the objective of this work was to induce shoots and roots in hypocotyls and cotyledons of P. peruviana L. using auxins and cytokinins. Nine combinations were made between the concentrations of 2,4-D (0.25, 0.5 and 1.0 mg / l) and BAP (0.1, 0.25 and 1.0 mg / l), in which 20-day-old explants were introduced. The basal medium was that of Murashige & Skoog supplemented with 3% sucrose, phytagel (0.5%) and pH 6. After 70 da...

Physalis peruviana L. "aguaymanto" is a Solanaceae highly prized in countries such as Peru, Colombia and Ecuador due to its high nutritional value and numerous medicinal properties. Its cultivation has high variability because it is propagated by seeds, which makes it necessary to maintain its homogeneous morphological and productive characteristics. An alternative is in vitro micropropagation to subsequently achieve its technification and industrialization. Therefore, the objective of this work was to induce shoots and roots in hypocotyls and cotyledons of P. peruviana L. using auxins and cytokinins. Nine combinations were made between the concentrations of 2,4-D (0.25, 0.5 and 1.0 mg / l) and BAP (0.1, 0.25 and 1.0 mg / l), in which 20-day-old explants were introduced. The basal medium was that of Murashige & Skoog supplemented with 3% sucrose, phytagel (0.5%) and pH 6. After 70 da...

Physalis peruviana L. “goldenberry” is a solanaceous that because of its high nutritional value and medicinal properties has been gained great importance in countries such as Ecuador, Colombia and Peru. This cultivation presents high variability owing to their main method of propagation involves the use of seeds. Therefore, is necessary to develop a protocol that allows its mass production maintaining homogeneous characteristics. In this sense, the objective of this research was regenerate plants from in vitro cultures of hypocotyls and cotyledons of P. peruviana using different concentrations of 2,4-D (0.25, 0.5 y 1.0 mg/L) and BAP (0.1, 0.25 y 1.0 mg/L). Callus formation was induced in all treatments in both hypocotyls and cotyledons, obtaining up to 100% of callogenesis in T4 (0.25 mg / L of 2,4-D + 0.1 mg / L of BAP). Root induction was obtained in treatments T7, T8 and T9 using ...

El aluminio (Al) es el tercer metal más abundante en la corteza terrestre y representa cerca del 8% de la misma. Una gran proporción es incorporada al suelo mineral como aluminisilicatos, con pequeñas cantidades en forma soluble - 1 mg l-1 (~ 37 μM) a valores de pH mayor de 5 pero su concentración sube a pH menores-  y junto con este son capaces de influenciar en los sistemas biológicos. Este metal tiene un efecto tóxico en el crecimiento de la planta sea por su alta concentración en el suelo o en soluciones nutritivas. Con estos antecedentes se propuso conocer el efecto del aluminio y del pH en el crecimiento de raíces de Phaseolus vulgaris var. caballero en condiciones de laboratorio. Previo a los tratamientos con Al, las raíces fueron expuestas, por 24 horas, a CaCl2 (0,5 mM), pH 4,5 en 500 ml de agua destilada. Luego se expusieron a concentraciones crecientes de aluminio 1...

El aluminio (Al) es el tercer metal más abundante en la corteza terrestre y representa cerca del 8% de la misma. Una gran proporción es incorporada al suelo mineral como aluminisilicatos, con pequeñas cantidades en forma soluble - 1 mg l-1 (~ 37 μM) a valores de pH mayor de 5 pero su concentración sube a pH menores-  y junto con este son capaces de influenciar en los sistemas biológicos. Este metal tiene un efecto tóxico en el crecimiento de la planta sea por su alta concentración en el suelo o en soluciones nutritivas. Con estos antecedentes se propuso conocer el efecto del aluminio y del pH en el crecimiento de raíces de Phaseolus vulgaris var. caballero en condiciones de laboratorio. Previo a los tratamientos con Al, las raíces fueron expuestas, por 24 horas, a CaCl2 (0,5 mM), pH 4,5 en 500 ml de agua destilada. Luego se expusieron a concentraciones crecientes de aluminio 1...