El objetivo de la presente investigación fue determinar el conocimiento de las enfermeras sobre los cuidados en fototerapia a neonatos con ictericia. Hospital de Emergencias Villa el Salvador ,2019. La investigación se fundamente de acuerdo con lo indicado por la OMS, donde considera al recién nacido hasta los 28 días de vida, donde comporta un mayor riesgo de muerte, por eso motivo es esencial brindar cuidados necesarios para su próxima existencia. La investigación fue de tipo básica, de nivel descriptivo, de enfoque cuantitativo, no experimental y de corte transversal. La muestra estuvo conformada por 42 enfermeras del servicio de neonatología del Hospital de Emergencias Villa el Salvador. La técnica utilizada fue la encuesta; el instrumento, un cuestionario de 20 ítems. El procesamiento de datos se llevó a cabo mediante el programa Excel y el SPSS-25. Los resultados indicar...

La presente investigación tiene como objetivo general determinar el potencial exportador de la empresa Corporación Enercon S.A.C ubicada en San Pedro de LLoc para facilitar el ingreso de papayas frescas hacia el mercado de Chile, Trujillo 2020. Se utilizó el diseño investigación no experimental, de nivel transversal y tipo descriptiva que permitió analizar la situación de la empresa para conocer el potencial exportador que posee para incursionar en la exportación. Para la obtención de la información, la técnica que se aplicó fue una encuesta aplicada a 3 personas que laboran en la empresa, el instrumento que se utilizó fue un cuestionario dividido en cuatro dimensiones: gestión administrativa, gestión productiva y logística, gestión de mercados internacionales - logística exportadora y gestión económica -financiera. Para su medición se utilizó la escala de Likert, d...

El presente trabajo tuvo como objetivo realizar una revisión sistemática de la literatura científica sobre el uso de tecnologías biotecnológicas en el tratamiento de residuos sólidos urbanos en América Latina, en el cual el tipo de investigación fue la investigación aplicada y el diseño sistemático, no empírico, analítico bibliográfico, observacional y retrospectivo. Obteniendo en los resultados, que las tecnologías biotecnológicas utilizadas en el tratamiento de residuos sólidos urbanos en América Latina son la digestión anaerobia, lixiviación, biorremediación, gasificación y compostaje. Las tecnologías biotecnológicas presentan media y alta eficiencia; siendo la biorremediación aquella que presentó los más elevados porcentajes de eficiencia, seguida de la gasificación, digestión anaerobia y el compostaje. Las limitaciones y desafíos que enfrenta la aplicac...

Hoy en día podemos apreciar que la Cultura y la conciencia tributaria son factores muy importantes para que la ciudadanía no cometa delitos tributarios; y por ende podamos comprender que los tributos son recursos que recauda el Estado en carácter de administrador, pero en realidad esos recursos le pertenecen a la población. La presente investigación tiene como objetivo Identificar las características de la Cultura Tributaria en los contribuyentes del Centro Comercial Plaza Vitarte, y como esto influye en la economía nacional, pero sobre todo a tener claro que la cultura tributaria no solo es saber cuáles son las obligaciones como contribuyente sino que debemos aplicar conocimientos en el complimiento de los deberes tributarios como buen contribuyente. Opte por este tema debido a la convivencia diaria en la sociedad, y el evidente desconocimiento o falta de interés en los comerci...

El presente trabajo de investigación fue desarrollado en av. Los Diamantes entre, av. Próceres hasta la av. Ramón Romero de la urb Bello Horizonte- Piura en el mes de diciembre 2017, el problema se presenta específicamente por el deterioro en los pavimentos intertrabado, por tanto, el objetivo principal de esta investigación es determinar y evaluar las patologías existentes en el pavimento intertrabado de la av. los Diamantes entre la av. los Próceres y la av. Ramón Romero- de la urb Bello Horizontes -Piura aplicando el método del (ICP) Los objetivos específicos fueron determinar los tipos de patologías que existen en el pavimento intertrabado de la de la av. Los Diamantes entre la av. los Próceres y la av. Ramón Romero- Piura y obtener el estado actual y condición de servicio La metodología utilizada para esta investigación fue descriptiva, analítica y no experimental. ...

En la presente monografía, haré un recorrido evaluativo del desarrollo de la Jornada escolar completa (JEC) teniendo como escenario mi contexto y experiencia como docente en una escuela JEC de la zona urbana en la I, E “María Auxiliadora de Sullana y una escuela JEC rural I.E María Luisa Seminario Campos del caserío de La Margarita de Querecotillo - Sullana” como directivo encargado. El Ministerio de Educación crea el servicio educativo de Jornada Escolar Completa Con la finalidad de ofrecer a las y los estudiantes del nivel secundario una mejor calidad del servicio educativo que recibían, proporcionándoles las herramientas necesarias para un mejor desarrollo de sus competencias y de esta manera acortar las brechas educativas existentes en nuestro país.

Since the industrial revolution, the science and technology advances have increased the human ability to exploit natural resources causing pollution in ecosystems. In this context, the use of biosurfactants represents a promising alternative application for any technological process of remediation of natural environments. The objective of this work was the evaluation of the emulsifying activity and the ability to remove heavy metals with a rhamnolipidic biosurfactant produced by Pseudomonas aeruginosa PB25. This strain had a specific growth rate (μ) of 0.0285 h-1 and a generational time (td) of 24.321 h. It produced 2.47 g/L rhamnolipid, with yields (Y) of 0.13 g/g and productivity of 0.082 g/L-h. The rhamnolipid had 5.257 emulsifying activity units/mL and E24 emulsification index of 53, 64, 62 and 84% for crude oil, diesel oil 2, gasoline and kerosene, respectively. It got to remove 98...

In this paper, we describe the isolation and biodegradation activity of microorganisms on low density polyethylene. The microorganisms were collected from plastic materials with evidence of deterioration from a landfill. The samples were filtered and selected in a mineral salts medium at pH 5.5 and 7 for bacteria and fungi respectively. Six strains were isolated, identified as Pseudomonas sp. Hyalodendron sp., Penicillium sp. and Rhodotorula sp. Microbial activity was evidenced by changes in the infrared spectrum of polyethylene with respect to the polymer without treatment. Reduction of carbonyl index (83.89% at pH 7 and 4.08% at pH 5.5) and double bonds index (19.77% at pH 7 and 6.47% at pH 5.5) were observed. Finally we determined the percentage of weight lost by the polyethylene subjected to activity of the strains, with a decrease of 5.4% at pH 7 and 4.8% at pH5, 5.

An L-aminoacid oxidase was purified from Bothrops brazili snake venom using Sephadex G-100 and CM-Sepahdex C?50 chromatographic method; in both cases 0,1M ammonium acetate pH 6 was used as a eluted. The enzyme was purified 29,3 fold with 30,9% of yield. A homogeneus protein band was achieved by PAGE-SDS, inmunodifusion as well as immunoelectrophoresis. The enzyme was an acid glicoprotein with a molecular weight of 125,7 kd consisting of two subunits of 59,91 kd each, linked by noncovalent bonds. The optimum pH was in the range of 7,5 to 9,0 depending on the L-aminoacid used as substrate. It was a thermoestable protein untill 55 oC, but its activity decreased by alcaline treatment. On the other hand, antibacterial assays using the Grove's method demonstrated that the whole venom as well as its purified enzyme produced a severe action on standardized grown cultures of Staphylococcus aureus...

The specificity between bacteriophages and bacteria is a feature that has been successfully used for the detection of several microbial species. For this reason the detection of vibriophages is a valuable research tool, and it might be a fast and convenient method to Vibrio cholerae diagnostics. The goal of this study was to detect vibriophages in shallow seawater samples and to determine the morphological characteristics of these vibriophages. It was determined the growth kinetics of a strain of Vibrio cholerae serotype Inaba. Seawater samples taken from five shallow points from an adjacent sector to La Chira beach, and Rímac and Chillón river mouths were analyzed qualitatively and quantitatively using different inoculum volumes and several incubation periods. The bacteriophages were concentrated and stained for the morphological study by transmission electron microscopy. The results ...

In this paper, we describe the isolation and biodegradation activity of microorganisms on low density polyethylene. The microorganisms were collected from plastic materials with evidence of deterioration from a landfill. The samples were filtered and selected in a mineral salts medium at pH 5.5 and 7 for bacteria and fungi respectively. Six strains were isolated, identified as Pseudomonas sp. Hyalodendron sp., Penicillium sp. and Rhodotorula sp. Microbial activity was evidenced by changes in the infrared spectrum of polyethylene with respect to the polymer without treatment. Reduction of carbonyl index (83.89% at pH 7 and 4.08% at pH 5.5) and double bonds index (19.77% at pH 7 and 6.47% at pH 5.5) were observed. Finally we determined the percentage of weight lost by the polyethylene subjected to activity of the strains, with a decrease of 5.4% at pH 7 and 4.8% at pH5, 5.

An L-aminoacid oxidase was purified from Bothrops brazili snake venom using Sephadex G-100 and CM-Sepahdex C?50 chromatographic method; in both cases 0,1M ammonium acetate pH 6 was used as a eluted. The enzyme was purified 29,3 fold with 30,9% of yield. A homogeneus protein band was achieved by PAGE-SDS, inmunodifusion as well as immunoelectrophoresis. The enzyme was an acid glicoprotein with a molecular weight of 125,7 kd consisting of two subunits of 59,91 kd each, linked by noncovalent bonds. The optimum pH was in the range of 7,5 to 9,0 depending on the L-aminoacid used as substrate. It was a thermoestable protein untill 55 oC, but its activity decreased by alcaline treatment. On the other hand, antibacterial assays using the Grove's method demonstrated that the whole venom as well as its purified enzyme produced a severe action on standardized grown cultures of Staphylococcus aureus...

The specificity between bacteriophages and bacteria is a feature that has been successfully used for the detection of several microbial species. For this reason the detection of vibriophages is a valuable research tool, and it might be a fast and convenient method to Vibrio cholerae diagnostics. The goal of this study was to detect vibriophages in shallow seawater samples and to determine the morphological characteristics of these vibriophages. It was determined the growth kinetics of a strain of Vibrio cholerae serotype Inaba. Seawater samples taken from five shallow points from an adjacent sector to La Chira beach, and Rímac and Chillón river mouths were analyzed qualitatively and quantitatively using different inoculum volumes and several incubation periods. The bacteriophages were concentrated and stained for the morphological study by transmission electron microscopy. The results ...

Use of biosurfactants in bioremediation, facilitates and accelerates microbial degradation of hydrocarbons. CTAB/MB agar method created by Siegmund & Wagner for screening of rhamnolipids (RL) producing strains, has been widely used but has not improved significantly for more than 20 years. To optimize the technique as a quantitative method, CTAB/MB agar plates were made and different variables were tested, like incubation time, cooling, CTAB concentration, methylene blue presence, wells diameter and inocula volume. Furthermore, a new method for RL detection within halos was developed: precipitation of RL with HCl, allows the formation a new halos pattern, easier to observe and to measure. This research reaffirm that this method is not totally suitable for a fine quantitative analysis, because of the difficulty to accurately correlate RL concentration and the area of the halos. RL dif...

Use of biosurfactants in bioremediation, facilitates and accelerates microbial degradation of hydrocarbons. CTAB/MB agar method created by Siegmund & Wagner for screening of rhamnolipids (RL) producing strains, has been widely used but has not improved significantly for more than 20 years. To optimize the technique as a quantitative method, CTAB/MB agar plates were made and different variables were tested, like incubation time, cooling, CTAB concentration, methylene blue presence, wells diameter and inocula volume. Furthermore, a new method for RL detection within halos was developed: precipitation of RL with HCl, allows the formation a new halos pattern, easier to observe and to measure. This research reaffirm that this method is not totally suitable for a fine quantitative analysis, because of the difficulty to accurately correlate RL concentration and the area of the halos. RL dif...

Genes associated to rhamnolipids production were molecularly characterized in 61 bacterial strains from LAMYBIM bacterial collection (Laboratorio de Microbiología y Biotecnología Microbiana, Universidad Nacional Mayor de San Marcos, Perú). Strains were isolated from peruvian environments hydrocarbons polluted and were classified as RL overproducers (n= 21), RL producers (n = 20) and non-producers (n = 20) producers. Molecular identification using the 16S rRNA gene was preceded by the biochemical identification of 61 strains selected with the API 20 NE system. Pseudomonas aeruginosa was the most prevalent strain of the RL overproducers and RL producers. Species such as Burkholderia cepacea, Pseudomonas fluorescens, Aeromonas hydrophila and Chryseobacterium indologenes, were found too. In the same way, non-producers microorganisms were also characterized. The PCR amplification and agaro...

Se realizó la caracterización molecular de los genes asociados a la producción de ramnolípidos (RL), en 61 cepas bacterianas de la colección del Laboratorio de Microbiología y Biotecnología Microbiana (LAMYBIM) de la Universidad Nacional Mayor de San Marcos, Perú. Las cepas provenian de entornos peruanos contami-nados con hidrocarburos y fueron catalogadas como sobreproductoras de RL(n= 21), productoras de RL (n= 20) y no productoras de RL (n= 20). Las 61 cepas fueron identificadas bioquímicamente con el sistema API 20 NE. La identificación molecular se realizó empleando el gen del RNAr 16S. Se encontró que Pseudomonas aeruginosa fue el microorganismo de mayor prevalencia en los estratos sobreproductores y productores de ramnolípidos. Además, se encontraron: Burkholderia cepacea, Pseudomonas fluorescens, Aeromonas hydrophila y Chryseobacterium indologenes. Los microorganism...