Antibiotics are used in cryopreservation of stallion semen in order to control the growth of microorganisms; however they can have negative effects on the spermatozoa. This study aimed to evaluate the effect of two antibiotic preparations on microbial growth and quality of cryopreserved stallion semen. Semen from 10 Colombian Creole horses was cryopreserved by conventional freezing with two antibiotic preparations: P1 (penicillin 10000 UI/ml and streptomycin 10 mg/ml) and P2 (penicillin 10000 UI/ml, streptomycin 10 mg/ml, and amphotericin B 25 μg/ml) and a control group without antibiotics. After thawing, computerized sperm motility evaluation, hypoosmotic test (HOS) and counting of bacteria and fungi in semen were performed. Statistical analysis was done using generalized linear models (GLM) and comparison of means by Tukey. Significant difference (p<0.05) for total motility (MT%) b...

The aim of this study was to evaluate three concentrations of centrifuged egg yolk in a commercial diluent for bovine semen. Ejaculates from four bulls were obtained and semen collections were made by electroejaculation and artificial vagina. The sperm obtained were divided into three aliquots supplemented with 10, 20 and 30% centrifuged egg yolk (YHC). Semen freezing was done in 0.5 ml straws. Thawed semen was evaluated for total (MT) and progressive motility (MP), vitality (VE), abnormal morphology (MA) and plasma membrane integrity (MI). Statistical analysis was performed by adjusting generalized linear models (GLM) and comparing means by the Tukey test. No significant differences were found for MT, VE, MA and IM between treatments, but there were significant differences for treatment with 10% YHC supplementation for linear (VSL), curvilinear (VCL) and mean (VAP) speeds with respect t...

Los antibióticos son utilizados en la criopreservación de semen equino con el fin de controlar la proliferación de microorganismos; sin embargo, estos pueden tener efectos negativos sobre los espermatozoides. La presente investigación tuvo como objetivo evaluar el efecto de dos preparaciones antibióticas sobre el desarrollo microbiano y la calidad de semen equino criopreservado. El semen de 10 caballos criollos colombianos fue criopreservado por congelación convencional, con dos preparaciones antibióticas: P1 (penicilina 10000 UI/ml y estreptomicina 10 mg/ml) y P2 (penicilina 10000 UI/ml, estreptomicina 10 mg/ml y anfotericina B 25 µg/ml) y un grupo control sin antibióticos. Posterior a la descongelación, se realizó la evaluación computarizada de la movilidad espermática, la prueba hipoosmótica (HOS) y el conteo de bacterias y hongos en el semen. El análisis estadístico s...

The aim of this study was to evaluate three concentrations of centrifuged egg yolk in a commercial diluent for bovine semen. Ejaculates from four bulls were obtained and semen collections were made by electroejaculation and artificial vagina. The sperm obtained were divided into three aliquots supplemented with 10, 20 and 30% centrifuged egg yolk (YHC). Semen freezing was done in 0.5 ml straws. Thawed semen was evaluated for total (MT) and progressive motility (MP), vitality (VE), abnormal morphology (MA) and plasma membrane integrity (MI). Statistical analysis was performed by adjusting generalized linear models (GLM) and comparing means by the Tukey test. No significant differences were found for MT, VE, MA and IM between treatments, but there were significant differences for treatment with 10% YHC supplementation for linear (VSL), curvilinear (VCL) and mean (VAP) speeds with respect t...