Brown algae are a source of high content of polysaccharides such as fucoidans that have important immunomodulatory properties. The aim was to determine the viability of human peripheral blood mononuclear cells (hPBMC), production of nitric oxide (NO), reactive oxygen species (ROS) and the proinflammatory cytokines IL-1α, IL-6, TNF-α and IFN -γ in cultures treated with fucoidan from Lessonia trabeculata. Fucoidan from Lessonia trabeculata from San Nicolás de Marcona-Ica Bay was used. The hPBMC were isolated using Ficoll-Hypaque, distributed at a concentration of 1x105 cells/well in complete RPMI-1640 medium and treated with different concentrations of fucoidan for 24 and 48 h. The cytotoxic activity was determined by the reduction of MTT, NO production by the Griess reaction and ROS by the reduction of NBT. The production of cytokines was quantified by ELISA. The fucoidan of L. trabec...

Brown algae are a source of high content of polysaccharides such as fucoidans that have important immunomodulatory properties. The aim was to determine the viability of human peripheral blood mononuclear cells (hPBMC), production of nitric oxide (NO), reactive oxygen species (ROS) and the proinflammatory cytokines IL-1α, IL-6, TNF-α and IFN -γ in cultures treated with fucoidan from Lessonia trabeculata. Fucoidan from Lessonia trabeculata from San Nicolás de Marcona-Ica Bay was used. The hPBMC were isolated using Ficoll-Hypaque, distributed at a concentration of 1x105 cells/well in complete RPMI-1640 medium and treated with different concentrations of fucoidan for 24 and 48 h. The cytotoxic activity was determined by the reduction of MTT, NO production by the Griess reaction and ROS by the reduction of NBT. The production of cytokines was quantified by ELISA. The fucoidan of L. trabec...

Determina la expresión génica transcripcional de inmunocitoquinas y el inmunofenotipo de las células mononucleares de sangre periférica humana (CMSPh) tratadas con la lectina semipurificada de Lupinus mutabilis Sweet, ecotipo Patón Grande. El extracto proteico (EPG) elaborado a partir de la harina desengrasada de semillas de lupino se fraccionó mediante cromatografía de exclusión molecular. Las lectinas del extracto y fracciones se identificaron mediante la actividad hemaglutinante y SDS-PAGE. Las CMSPh se incubaron durante 24 y 72 h con el EPG (5 μg/mL), la fracción 9 del pico I (F9-PI 5 y 25 μg/mL), fitohemaglutinina (PHA, control positivo) y medio RPMI (control negativo). La expresión génica de las inmunocitoquinas e inmunofenotipificación se realizó mediante la PCR convencional y citometría de flujo respectivamente. El EPG y la F9-PI a la concentración de 5 μg/mL du...

Los fucoidanos son compuestos obtenidos de algas pardas con importantes funciones bioactivas. El objetivo fue estudiar in vitro la propiedad inmunomoduladora y antitumoral del fucoidan de Lessonia trabeculata (FLt) sobre células mononucleares de sangre periférica humana (CMSPh) y líneas celulares de cáncer de cérvix (HeLa), leucemia promonocítica humana (U937). Las células fueron tratadas con diferentes concentraciones de FLt (PSW S.A.), fucoidan comercial de Fucus vesiculosus (FFv, control fucoidan) y Doxorrubicina (Dox, control de citotoxicidad). La viabilidad de las CMSPh se evaluó por el ensayo del MTT (48 h), mientras que la síntesis de especies reactivas de nitrógeno (NO, 24-48 h) y oxígeno (ROS, 24-48 h) se detectaron por la reacción de Griess y NBT respectivamente. La expresión génica de las citoquinas proinflamatorias IL-1α, IL-6, TNF-α e IFN-Ȗ se determinó por...

Neoplastic tumors are characterized by invasiveness and metastasis.  Neoplasticcells are genetically heterogeneous and can develop resistance to chemotherapeutic agents. For this reason, plants continue to be an important source of new anticancer products. Objective. To determine the cytotoxic and antiproliferative activity of a fucoidan-rich extract of native Lessonia trabeculata (EFLt) on the tripe negative murine mammary adenocarcinoma cell line 4T1. Methods. Cytotoxicity and IC50 were determined in 4T1 monolayers using the MTT reagent. To demonstrate antiproliferative activity, “wound” closure and anticlonogenic methods were applied using the IC50 of EFLt and Doc (doxorubicin). “Wound” closure was evaluated by discrete times sweep to determine percentage inhibition of cell migration; the anticlonogenic effect was evaluated by colony counting 7 days after treatment and the su...

Fasciolosis is a cosmopolitan parasitosis medical-veterinary importance caused by Fasciola hepatica, which affects sheep, goats and cattle; and it affects man accidentally causing an epidemic-endemic infection difficult to diagnose. The aim was to develop an indirect sandwich ELISA with 3 antibodies for detecting excretory-secretory antigens of Fasciola hepatica (ESFh). For the development of indirect sandwich ELISA were used, as capture antibody, mouse polyclonal antibodies anti ESFh and polyclonal antibodies rabbit anti-ESFh as detection antibody, at the concentrations of 10 and 5 µg/mL respectively. The conjugate used was mouse monoclonal anti- total immunoglobulins rabbit linked to peroxidase (1/1000). Were analized 31 sheep fecal samples, and the results were compared with those obtained by direct coproparasitological examination (DC) and counterimmunoelectrophoresis (CIEP). The de...

La fasciolosis es una parasitosis cosmopolita de importancia médico-veterinaria ocasionada por Fasciola hepatica, que afecta al ganado ovino, caprino y vacuno; y accidentalmente al hombre ocasionando una infección endemo-epidémica de difícil diagnóstico. El objetivo fue desarrollar un ELISA sándwich indirecto, empleando 3 anticuerpos, para identificar antígenos de secreción-excreción de Fasciola hepatica (ESFh). Para el ELISA se emplearon anticuerpos policlonales de ratón anti ESFh como anticuerpos de captura, y anticuerpos policlonales de conejo anti ESFh como anticuerpos de detección, a las concentraciones de 10 y 5 µg/mL, respectivamente. Como conjugado se emplearon anticuerpos monoclonales de ratón anti-inmunoglobulinas totales de conejo ligado a peroxidasa (1/1000). Se analizaron 31 muestras de heces de ganado ovino y los resultados se compararon con los obtenidos por e...

Acute stress is related to the energetic release of catecholamines, cytokines, and cortisol, which trigger pain perception. Chewing stimulates serotonergic neurons, these neurons modulate the neural pathways of pain; however, the relationship between these variables is still in the process of understanding. Objective. To determine the effect of acute stress and chewing stimulus on pain. Methods. an experiment that was developed in the Faculty of Medicine of the UNMSM. 40 eight-week-old male Balb/c mice were randomly assigned into 5 equal groups. A: week 1 without any stimulus and week 2 chewing stimulus + stress. B: week 1 without any stimulus and week 2 stress. C: week 1 stress and week 2 without any stimulus. D: week 1 masticatory stimulus + stress and week 2 without any stimulus. E: control, without any stimulus. Pain was assessed in the 5 groups at 7 and 14 days using the tail-withdr...

Introduction. Acute stress alters memory and spatial learning and the expression of interleukin 6, the chewing stimulus would prevent these effects. Objective. To determine the effect of chewing stimulation and acute stress on the expression of interleukin 6 and memory and spatial learning in mice. Methods. Experiment where 70 male albino mice of the Balb/c of age 2 month were randomly distributed into: Group A1: acute stress 1 hour; Group A2: acute stress 1 hour + chewing stimulus 1 hour; Group B1: acute stress 2 hours; Group B2: acute stress 2 hours + chewing stimulus 2 hours; Group C1: acute stress 3 hours; C2: acute stress 3 hours + chewing stimulus 3 hours; Group D: without intervention. For 3 days, spatial memory and learning were tested in the Morris water maze. Interleukin 6 (IL-6) was analyzed by ELISA test. Results. IL-6 was higher in the B2 group vs the other groups (p<0.00...