The ecosystem service of water regulation is one of the most important offered by the high[1]Andean ecosystems. However, knowledge about its contribution in hydrological terms is still scarce and its estimation is difficult, due to the complex eco-hydrological and climatic processes, and environmental characteristics of the Andes. Therefore, the influence of three types of ecosystems (humid puna grassland, tropical high-Andean wetland (bofedal) and Polylepis forest) was estimated on water flows, particularly flows that reach underground storage, which is equivalent to the ecosystem service of water regulation. This study was developed during the hydrological year 2018 - 2019 in the Rontoccocha hydrographic unit, between 3 900 and 4 635 masl, in the Apurimac Region, Peru. For this, the water balance for each type of ecosystem was modeled with the Hydrobal eco-hydrological tool. Variables ...