Las células madre tienen capacidad de auto renovarse mediante divisiones mitóticas o bien continuar la vía de diferenciación para la que están programadas. El potencial terapéutico que tienen las células madre originando múltiples linajes, en la aplicación de terapia celular, ingeniería de tejidos y terapia génica, es de gran relevancia. El tejido adiposo es otra fuente alternativa para aislar células madre mesénquimales (ASC). Se describe la técnica convencional de lipoaspiración (disrupción mecánica), y se presenta la experiencia de una nueva técnica con aplicación de nuevas tecnologías estudiadas desde el 2010: Prototipo de laser diodo con longitud de onda 1210-nm. (desnaturalización del tejido conectivo), con claras diferencias cuantitativas y cualitativas de las células madre cosechadas, presentando una nueva propiedad que es la estimulación selectiva de las A...

Las células madre tienen capacidad de auto renovarse mediante divisiones mitóticas o bien continuar la vía de diferenciación para la que están programadas. El potencial terapéutico que tienen las células madre originando múltiples linajes, en la aplicación de terapia celular, ingeniería de tejidos y terapia génica, es de gran relevancia. El tejido adiposo es otra fuente alternativa para aislar células madre mesénquimales (ASC). Se describe la técnica convencional de lipoaspiración (disrupción mecánica), y se presenta la experiencia de una nueva técnica con aplicación de nuevas tecnologías estudiadas desde el 2010: Prototipo de laser diodo con longitud de onda 1210-nm. (desnaturalización del tejido conectivo), con claras diferencias cuantitativas y cualitativas de las células madre cosechadas, presentando una nueva propiedad que es la estimulación selectiva de las A...

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El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.

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Introducción y Objetivo. Los avances tecnológicos en los pro- cedimientos de Cirugía Plástica han favorecido la obtención de bue- nos resultados evitando un trauma quirúrgico mayor y disminuyendo las complicaciones postoperatorias. En este sentido, el uso de la tec- nología láser aplicado a la liposucción supuso una herramienta muy interesante e introdujo cambios sustanciales en la metodóloga y posi- bilidades de esta intervención Presentamos nuestra experiencia con el uso del láser 980-nm y de una nueva tecnología de láser 1210-nm empleadas en combinación con la cirugía de lipoabdominoplastia. Material y Método. Realizamos un estudio retrospectivo en pa- cientes intervenidos con nuestro protocolo quirúrgico entre los años 2007 y 2014. Realizamos la lipoaspiración en un primer periodo con láser de 980-nm y posteriormente el de 1210-nm, previa a lipoabdomi- noplastia ...

INTRODUCTION: The 1210-nm wavelength has absorption-affinity for Lipid-Rich Tissue and has demonstrated to stimulate adipocytes and mesenchymal cells of the subcutaneous tissue, with a new concept: Selective Photothermostimulation (SPS). The application in Liposuction of this wavelength is determined by its preservation of the integrity of adipocytes, making possible the use of these materials for fat grafting in Liposculpture, Breast Reconstruction or as filler in Face Rejuvenation, proven that this technique is less traumatic. MATERIALS AND METHODS: 102 patients diagnosed with lipodystrophy either associated to skin flaccidity or not were applied the 1210-nm diode laser (ORlight®) between June 2010 and August 2011. The protocol was followed and verified through ultrasound imaging. Intraoperative and subcutaneous cellular tissue temperatures were measured. Samples of the aspirated tiss...

Background: Facial fat grafting for rejuvenation is one of the most popular facial aesthetic procedures in plastic surgery. It is always challenging and since there are a lot of techniques for adipose tissue (AT) harvesting, there are no standard procedures that guarantee natural and long-lasting results. We developed the selective tissue engineering photo stimulation technique (One STEP™) in which we used a novel infrared 1210-nm wavelength laser diode for fat preserved harvesting and direct fat injection that we named PicoGraft™, with no fat manipulation. Methods: This is a retrospective descriptive study in which we included all senior author’s patients that got facial fat grafting using the One STEP™ technique. We compared the AT aspirated, after laser emission (STEP-PicoGraft) and the standard assisted liposuction samples (SAL) in cultures. We study the mitochondrial activit...

BACKGROUND: The understanding of facial anatomy and its changes through aging has led to the development of several different facelift techniques that focus on being less invasive and traumatic and, at the same time, providing natural long-lasting results. In this article we describe step by step our facelift technique as it has been done over the past 10 years by the senior author. METHODS: This is a retrospective, descriptive, transversal study in which all patients who underwent a rhytidectomy using our technique from January 2002 to September 2012 were included. All patients were operated on under local anesthesia and superficial conscious sedation. All surgeries were performed by the same surgeon. A complete step-by-step description of the surgical technique can be found in the main article. RESULTS: Between January 2002 and September 2012, a total of 113 patients underwent facelift...

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Introduction: Liposuction has undergone several improvements since its first description, including changes in the cannulas, variation in the concentration of the infiltrating solution, and the use of different devices and technologies. The use of laser technology devices for lipolysis and stimulation of skin retraction has contributed to the procedure. This article presents the authors’ experience with laser lipolysis in 400 patients, within a 5-year period, and discusses the principles of the technology and its effect on tissues. Methods: This is a study performed between July 2007 and July 2012 and included 400 patients who underwent laser lipolysis. All procedures were performed following the original protocol – infiltration of cold saline, passage of the cannula with an optic fiber for conducting the energy needed for laser lipolysis, skin retraction, and finally, conventional l...

Intestinal Barrier and Intestinal Permeability The "Intestinal Barrier" (BI) covers an area of approximately 400 m2 and requires about 40% of theenergy expenditure of the body. Among its functions are to avoid the loss of water and electrolytes and the entry of antigens and microorganisms to the human organism1. At the same time, the BI must allow the exchange of molecules between the body and the environment, as well as the absorption of nutrients from food consumed. The specialized adaptations of the intestinal mucosa of the mammal perform two functions apparently opposite: first, allow a peaceful coexistence with the intestinal microbiota without cause chronic inflammation and, secondly, provide a defensive inflammatory and response measure according to the threat of pathogens    DOI 10.25176/RFMH.v18.n4.1725

Intestinal Barrier and Intestinal Permeability The "Intestinal Barrier" (BI) covers an area of approximately 400 m2 and requires about 40% of theenergy expenditure of the body. Among its functions are to avoid the loss of water and electrolytes and the entry of antigens and microorganisms to the human organism1. At the same time, the BI must allow the exchange of molecules between the body and the environment, as well as the absorption of nutrients from food consumed. The specialized adaptations of the intestinal mucosa of the mammal perform two functions apparently opposite: first, allow a peaceful coexistence with the intestinal microbiota without cause chronic inflammation and, secondly, provide a defensive inflammatory and response measure according to the threat of pathogens    DOI 10.25176/RFMH.v18.n4.1725