Las células madre tienen capacidad de auto renovarse mediante divisiones mitóticas o bien continuar la vía de diferenciación para la que están programadas. El potencial terapéutico que tienen las células madre originando múltiples linajes, en la aplicación de terapia celular, ingeniería de tejidos y terapia génica, es de gran relevancia. El tejido adiposo es otra fuente alternativa para aislar células madre mesénquimales (ASC). Se describe la técnica convencional de lipoaspiración (disrupción mecánica), y se presenta la experiencia de una nueva técnica con aplicación de nuevas tecnologías estudiadas desde el 2010: Prototipo de laser diodo con longitud de onda 1210-nm. (desnaturalización del tejido conectivo), con claras diferencias cuantitativas y cualitativas de las células madre cosechadas, presentando una nueva propiedad que es la estimulación selectiva de las A...

Las células madre tienen capacidad de auto renovarse mediante divisiones mitóticas o bien continuar la vía de diferenciación para la que están programadas. El potencial terapéutico que tienen las células madre originando múltiples linajes, en la aplicación de terapia celular, ingeniería de tejidos y terapia génica, es de gran relevancia. El tejido adiposo es otra fuente alternativa para aislar células madre mesénquimales (ASC). Se describe la técnica convencional de lipoaspiración (disrupción mecánica), y se presenta la experiencia de una nueva técnica con aplicación de nuevas tecnologías estudiadas desde el 2010: Prototipo de laser diodo con longitud de onda 1210-nm. (desnaturalización del tejido conectivo), con claras diferencias cuantitativas y cualitativas de las células madre cosechadas, presentando una nueva propiedad que es la estimulación selectiva de las A...

BACKGROUND: The understanding of facial anatomy and its changes through aging has led to the development of several different facelift techniques that focus on being less invasive and traumatic and, at the same time, providing natural long-lasting results. In this article we describe step by step our facelift technique as it has been done over the past 10 years by the senior author. METHODS: This is a retrospective, descriptive, transversal study in which all patients who underwent a rhytidectomy using our technique from January 2002 to September 2012 were included. All patients were operated on under local anesthesia and superficial conscious sedation. All surgeries were performed by the same surgeon. A complete step-by-step description of the surgical technique can be found in the main article. RESULTS: Between January 2002 and September 2012, a total of 113 patients underwent facelift...

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Background and Objective. Technological advances in the pro- cedures of Plastic Surgery have favored the obtaining of good results avoiding a greater surgical trauma and reducing the postoperative complications. In this sense, the use of laser technology applied to li- posuction was a very interesting tool and introduced substantial chan- ges in the methodology and possibilities of this intervention We present our experience with the use of 980-nm laser and new 1210-nm laser technology in combination with lipoabdominoplasty surgery. Methods. We conduct a retrospective study in patients operated with our surgical protocol between 2007 and 2014. We performed li- posuction in a first period with 980-nm laser and then 1210-nm laser, prior to lipoabdominoplasty with classical technique and reduced scar. We did 101 lipoabdominoplasties, 47 with the 980-nm laser and 54 the 1210-nm laser, with a...

Diode Laser (LSDL) 1210-nm is ideal for Liposculpture.

Background: Facial fat grafting for rejuvenation is one of the most popular facial aesthetic procedures in plastic surgery. It is always challenging and since there are a lot of techniques for adipose tissue (AT) harvesting, there are no standard procedures that guarantee natural and long-lasting results. We developed the selective tissue engineering photo stimulation technique (One STEP™) in which we used a novel infrared 1210-nm wavelength laser diode for fat preserved harvesting and direct fat injection that we named PicoGraft™, with no fat manipulation. Methods: This is a retrospective descriptive study in which we included all senior author’s patients that got facial fat grafting using the One STEP™ technique. We compared the AT aspirated, after laser emission (STEP-PicoGraft) and the standard assisted liposuction samples (SAL) in cultures. We study the mitochondrial activit...

pccenturion@gmail.com

pccenturion@gmail.com

Introduction: Liposuction has undergone several improvements since its first description, including changes in the cannulas, variation in the concentration of the infiltrating solution, and the use of different devices and technologies. The use of laser technology devices for lipolysis and stimulation of skin retraction has contributed to the procedure. This article presents the authors’ experience with laser lipolysis in 400 patients, within a 5-year period, and discusses the principles of the technology and its effect on tissues. Methods: This is a study performed between July 2007 and July 2012 and included 400 patients who underwent laser lipolysis. All procedures were performed following the original protocol – infiltration of cold saline, passage of the cannula with an optic fiber for conducting the energy needed for laser lipolysis, skin retraction, and finally, conventional l...

Intestinal Barrier and Intestinal Permeability The "Intestinal Barrier" (BI) covers an area of approximately 400 m2 and requires about 40% of theenergy expenditure of the body. Among its functions are to avoid the loss of water and electrolytes and the entry of antigens and microorganisms to the human organism1. At the same time, the BI must allow the exchange of molecules between the body and the environment, as well as the absorption of nutrients from food consumed. The specialized adaptations of the intestinal mucosa of the mammal perform two functions apparently opposite: first, allow a peaceful coexistence with the intestinal microbiota without cause chronic inflammation and, secondly, provide a defensive inflammatory and response measure according to the threat of pathogens    DOI 10.25176/RFMH.v18.n4.1725

Intestinal Barrier and Intestinal Permeability The "Intestinal Barrier" (BI) covers an area of approximately 400 m2 and requires about 40% of theenergy expenditure of the body. Among its functions are to avoid the loss of water and electrolytes and the entry of antigens and microorganisms to the human organism1. At the same time, the BI must allow the exchange of molecules between the body and the environment, as well as the absorption of nutrients from food consumed. The specialized adaptations of the intestinal mucosa of the mammal perform two functions apparently opposite: first, allow a peaceful coexistence with the intestinal microbiota without cause chronic inflammation and, secondly, provide a defensive inflammatory and response measure according to the threat of pathogens    DOI 10.25176/RFMH.v18.n4.1725