The aim of this study was to discover alpaca SNPs by genotyping forty alpaca DNA samples using the BovineHD Genotyping Beadchip. Data analysis was performed with GenomeStudio, Illumina, software. Because different filters and thresholds are reported in the literature we investigated the effects of no-call threshold and call frequency in identifying positive SNPs. Unique SNPs identified in both reference genomes were kept and mapped on the Vicugna_pacos 2.0.2 genome.