The aim of this study was to evaluate the relative expression of the cytokines involved in the immune response of Th1 (IL-2, IL-12, IFN-γ, TNF-α), Th2 (IL-4, IL-10 and TGF-β) and Th17 (IL-17) in 21 guinea pigs (Cavia porcellus) experimentally inoculated with a field isolate of Salmonella Typhimurium at a dose of 102 CFU/ml, intraperitoneally, and compared with a control group (inoculated with a thermally treated strain). Blood samples were taken on days 1, 3, 5, 7, 9, 15 and 30 post-inoculation (p.i.). Total RNA was extracted from lymphocyte cells and real-time RT-PCR was conducted using specific primers for each of the cytokines. The relative mRNA expressions were determined by the comparative method 2-ΔΔCt with respect to the calibrator (healthy guinea pig) and using GAPDH as a constitutive gene of reference as a normalizer. The expressions of the cytokine genes in the Treatment g...

The aim of this study was to evaluate the relative expression of the cytokines involved in the immune response of Th1 (IL-2, IL-12, IFN-γ, TNF-α), Th2 (IL-4, IL-10 and TGF-β) and Th17 (IL-17) in 21 guinea pigs (Cavia porcellus) experimentally inoculated with a field isolate of Salmonella Typhimurium at a dose of 102 CFU/ml, intraperitoneally, and compared with a control group (inoculated with a thermally treated strain). Blood samples were taken on days 1, 3, 5, 7, 9, 15 and 30 post-inoculation (p.i.). Total RNA was extracted from lymphocyte cells and real-time RT-PCR was conducted using specific primers for each of the cytokines. The relative mRNA expressions were determined by the comparative method 2-ΔΔCt with respect to the calibrator (healthy guinea pig) and using GAPDH as a constitutive gene of reference as a normalizer. The expressions of the cytokine genes in the Treatment g...