The β-galactosidases (EC3.2.1.23) are glycosyl hydrolases which mainly catalyze the hydrolysis of β-D-galactosides in various types of microorganisms, which are used in the synthesis of oligosaccharides. One of the potential sources for the production of enzymes that show these characteristics are microorganisms from extreme environments, such as salt brine. In this study, was cloned and characterized the betagalactosidase gene from Bacillus sp MSP7 isolated from Pilluana saltern in San Martin, Peru, because it shown enzymatic activity of 65 U/mg dried cells, the greatest activity than other four Bacillus sp. strains isolated from same village. For this, specific primers were designed from regions of consensus amino acid sequences of the Bacillus genus from available betagaltosidase sequences in data banks. Betagalactsidase gene of Bacillus sp. MSP7 was amplified by polymerase chain re...

The β-galactosidases (EC3.2.1.23) are glycosyl hydrolases which mainly catalyze the hydrolysis of β-D-galactosides in various types of microorganisms, which are used in the synthesis of oligosaccharides. One of the potential sources for the production of enzymes that show these characteristics are microorganisms from extreme environments, such as salt brine. In this study, was cloned and characterized the betagalactosidase gene from Bacillus sp MSP7 isolated from Pilluana saltern in San Martin, Peru, because it shown enzymatic activity of 65 U/mg dried cells, the greatest activity than other four Bacillus sp. strains isolated from same village. For this, specific primers were designed from regions of consensus amino acid sequences of the Bacillus genus from available betagaltosidase sequences in data banks. Betagalactsidase gene of Bacillus sp. MSP7 was amplified by polymerase chain re...