Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin

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Objetive. Standardize a new immunohistochemical protocol to identify Helicobacter pylori in gastric biopsies. Materials and Methods: the work was carried out in three phases. The first phase makes use of polyclonal rabbit Anti-H. pylori antibodies as a primary antibody for the labeling of said bacte...

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Autores: Mejía-Parra, Jorge Luis Jhoao, Guerrero-Espinoza, Andric E., Flores-Arrascue, Carlota P., Chiclayo-Padilla, Alfredo S.
Formato: artículo
Fecha de Publicación:2020
Institución:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Repositorio:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Lenguaje:español
OAI Identifier:oai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/740
Enlace del recurso:https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/740
Nivel de acceso:acceso abierto
Materia:Inmunohistoquimica
Helicobacter pylori
gastritis
cambios histológicos pre malignos
Immunohistochemistry
pre-malignant histological changes
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spelling Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-EosinEstandarización de nuevo protocolo Inmunohistoquímico para identificar Helicobacter pylori de biopsias gástricas y valoración frente a la tinción Hematoxilina-EosinaMejía-Parra, Jorge Luis JhoaoGuerrero-Espinoza, Andric E.Flores-Arrascue, Carlota P.Chiclayo-Padilla, Alfredo S.InmunohistoquimicaHelicobacter pylorigastritiscambios histológicos pre malignosImmunohistochemistryHelicobacter pylorigastritispre-malignant histological changesObjetive. Standardize a new immunohistochemical protocol to identify Helicobacter pylori in gastric biopsies. Materials and Methods: the work was carried out in three phases. The first phase makes use of polyclonal rabbit Anti-H. pylori antibodies as a primary antibody for the labeling of said bacteria, in addition to an EnVision FLEX / HRP polymer for labeling in gastric biopsies. In the second, the immunostaining contrast was modified with the Harris Hematoxylin and Eosin Y dyes, in order to more clearly appreciate the cytomorphology of the gastric tissue to be examined and the possible pre-malignant histological changes. Finally, the results were evaluated against Hematoxylin and Eosin (H&E) staining. Results and Discussion: We show that by carrying out the new immunohistochemistry protocol, it facilitates the identification of H. pylori and, in turn, allows the evaluation of cytomorphological changes in gastric biopsies. The establishment of this protocol will allow an adequate study of the association of H. pylori infection and pre-malignant histological changes in gastric tissue.Objetivo. Estandarizar un nuevo protocolo de inmunohistoquímica para identificar Helicobacter pylori, en biopsias gástricas. Material y Métodos: el trabajo se realizó en tres fases. La primera fase hace uso de anticuerpos policlonales de conejo Anti-H. pylori como anticuerpo primario para la marcación de dicha bacteria, además de un polímero EnVision FLEX/HRP para la marcación en biopsias gástricas. En la segunda se modificó el contraste de la inmunotinción con los colorantes Hematoxilina de Harris y Eosina Y, con la finalidad de apreciar con más claridad la citomorfología del tejido gástrico a examinar y a los posibles cambios histológicos pre malignos. Por último se procedió a valorar los resultados frente a la tinción Hematoxilina y Eosina (H&E). Resultados y Discusión: Demostramos que al realizar el nuevo protocolo de inmunohistoquímica facilita la identificación de H. pylori y a su vez permite evaluar los cambios citomorfológicos en las biopsias gástricas. El establecimiento de este protocolo permitirá estudiar adecuadamente la asociación de la infección por H. pylori y los cambios histológicos pre-malignos en el tejido gástrico.Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo2020-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/74010.35434/rcmhnaaa.2020.133.740Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 13 No. 3 (2020): Rev. Cuerpo Med. HNAAA; 291 - 298Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 13 Núm. 3 (2020): Rev. Cuerpo Med. HNAAA; 291 - 2982227-47312225-5109reponame:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstname:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstacron:HNAAAspahttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/740/360Derechos de autor 2020 Revista del Cuerpo Médico del HNAAAinfo:eu-repo/semantics/openAccessoai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/7402021-06-14T05:31:54Z
dc.title.none.fl_str_mv Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
Estandarización de nuevo protocolo Inmunohistoquímico para identificar Helicobacter pylori de biopsias gástricas y valoración frente a la tinción Hematoxilina-Eosina
title Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
spellingShingle Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
Mejía-Parra, Jorge Luis Jhoao
Inmunohistoquimica
Helicobacter pylori
gastritis
cambios histológicos pre malignos
Immunohistochemistry
Helicobacter pylori
gastritis
pre-malignant histological changes
title_short Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
title_full Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
title_fullStr Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
title_full_unstemmed Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
title_sort Standardization of the new immunohistochemical protocol to identify Helicobacter pylori from gastric biopsies and contrast against Hematoxylin-Eosin
dc.creator.none.fl_str_mv Mejía-Parra, Jorge Luis Jhoao
Guerrero-Espinoza, Andric E.
Flores-Arrascue, Carlota P.
Chiclayo-Padilla, Alfredo S.
author Mejía-Parra, Jorge Luis Jhoao
author_facet Mejía-Parra, Jorge Luis Jhoao
Guerrero-Espinoza, Andric E.
Flores-Arrascue, Carlota P.
Chiclayo-Padilla, Alfredo S.
author_role author
author2 Guerrero-Espinoza, Andric E.
Flores-Arrascue, Carlota P.
Chiclayo-Padilla, Alfredo S.
author2_role author
author
author
dc.subject.none.fl_str_mv Inmunohistoquimica
Helicobacter pylori
gastritis
cambios histológicos pre malignos
Immunohistochemistry
Helicobacter pylori
gastritis
pre-malignant histological changes
topic Inmunohistoquimica
Helicobacter pylori
gastritis
cambios histológicos pre malignos
Immunohistochemistry
Helicobacter pylori
gastritis
pre-malignant histological changes
description Objetive. Standardize a new immunohistochemical protocol to identify Helicobacter pylori in gastric biopsies. Materials and Methods: the work was carried out in three phases. The first phase makes use of polyclonal rabbit Anti-H. pylori antibodies as a primary antibody for the labeling of said bacteria, in addition to an EnVision FLEX / HRP polymer for labeling in gastric biopsies. In the second, the immunostaining contrast was modified with the Harris Hematoxylin and Eosin Y dyes, in order to more clearly appreciate the cytomorphology of the gastric tissue to be examined and the possible pre-malignant histological changes. Finally, the results were evaluated against Hematoxylin and Eosin (H&E) staining. Results and Discussion: We show that by carrying out the new immunohistochemistry protocol, it facilitates the identification of H. pylori and, in turn, allows the evaluation of cytomorphological changes in gastric biopsies. The establishment of this protocol will allow an adequate study of the association of H. pylori infection and pre-malignant histological changes in gastric tissue.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/740
10.35434/rcmhnaaa.2020.133.740
url https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/740
identifier_str_mv 10.35434/rcmhnaaa.2020.133.740
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/740/360
dc.rights.none.fl_str_mv Derechos de autor 2020 Revista del Cuerpo Médico del HNAAA
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Revista del Cuerpo Médico del HNAAA
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo
publisher.none.fl_str_mv Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo
dc.source.none.fl_str_mv Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 13 No. 3 (2020): Rev. Cuerpo Med. HNAAA; 291 - 298
Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 13 Núm. 3 (2020): Rev. Cuerpo Med. HNAAA; 291 - 298
2227-4731
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