Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies

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A fast, simple and reliable method for the extraction of DNA from Gossypium barbadense radioinduced mutants has been developed. This method is cetyltrimethylammonium bromide (cTAB) based, and it extracts DNA from seed leaves processed in a 1.5 mL tube with 0.7 mL of extraction buffer; is highly effi...

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Detalles Bibliográficos
Autores: Álvarez, Félix, Leyva, Omar, Osores, José
Formato: documento de trabajo
Fecha de Publicación:2008
Institución:Instituto Peruano de Energía Nuclear
Repositorio:IPEN-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.ipen.gob.pe:20.500.13054/562
Enlace del recurso:https://hdl.handle.net/20.500.13054/562
Nivel de acceso:acceso abierto
Materia:Extracción de adn
Mutantes inducidos por radiación
Algodón
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spelling Álvarez, FélixLeyva, OmarOsores, JoséLeyva, OmarÁlvarez, FélixOsores, José2016-07-18T17:22:16Z2016-07-18T17:22:16Z2008-11Álvarez F, Leyva O, Osores J. Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies. Informe Científico Tecnológico. 2007; 7: 183-186.1684-1662https://hdl.handle.net/20.500.13054/562A fast, simple and reliable method for the extraction of DNA from Gossypium barbadense radioinduced mutants has been developed. This method is cetyltrimethylammonium bromide (cTAB) based, and it extracts DNA from seed leaves processed in a 1.5 mL tube with 0.7 mL of extraction buffer; is highly efficient to cotton tissue and much cheaper in terms of time, chemical use and labor input. The described protocol presents consistent yield and good quality of DNA for several molecular analyses (AFLP, SSR, etc.).Se ha desarrollado un método rápido, simple y seguro para la extracción de ADN en mutantes radioinducidos de Gossypium barbadense. Este método se basa en el bromuro de cetyltrimetil amonio y extrae ADN de hojas primordiales procesadas en tubos de 1.5 mL con 0.7 mL de buffer de extracción; es altamente eficiente para tejidos de algodón y mucho más económico en términos de tiempo, uso de químicos y labores. El protocolo descrito presenta consistente rendimiento y buena calidad de ADN para varios análisis moleculares (AFLP, SSR, etc.).Instituto Peruano de Energía Nuclearapplication/pdfengLima (Perú)PEinfo:eu-repo/semantics/openAccessInstituto Peruano de Energía NuclearRepositorio Institucional del Instituto Peruano de Energía Nuclearreponame:IPEN-Institucionalinstname:Instituto Peruano de Energía Nuclearinstacron:IPENExtracción de adnMutantes inducidos por radiaciónAlgodónGenomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studiesinfo:eu-repo/semantics/workingPaperORIGINALPag 183-186 ICT-2007.pdfPag 183-186 ICT-2007.pdfapplication/pdf54220https://repositorio.ipen.gob.pe/bitstream/20.500.13054/562/1/Pag%20183-186%20ICT-2007.pdfd899985c0171f312e3e821d7a5fdbf1bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-8103https://repositorio.ipen.gob.pe/bitstream/20.500.13054/562/2/license.txt7ecf7b0639239de61a3a1ede9c397fecMD52TEXTPag 183-186 ICT-2007.pdf.txtPag 183-186 ICT-2007.pdf.txtExtracted texttext/plain11410https://repositorio.ipen.gob.pe/bitstream/20.500.13054/562/3/Pag%20183-186%20ICT-2007.pdf.txt46fe92ba54bb207d07c143b0a9c024dbMD53THUMBNAILPag 183-186 ICT-2007.pdf.jpgPag 183-186 ICT-2007.pdf.jpgGenerated Thumbnailimage/jpeg17876https://repositorio.ipen.gob.pe/bitstream/20.500.13054/562/4/Pag%20183-186%20ICT-2007.pdf.jpgc8a068fc437e4c29d2c37a1a83a09fe1MD5420.500.13054/562oai:repositorio.ipen.gob.pe:20.500.13054/5622022-02-07 18:08:53.022Repositorio Institucional del Instituto Peruano de Energía Nuclearrepositorio@ipen.gob.peU2UgRGlzdHJpYnV5ZSBiYWpvIHVuYSBMaWNlbmNpYSBDcmVhdGl2ZSBDb21tb25zIEF0cmlidWNpw7NuLU5vQ29tZXJjaWFsLVNpbkRlcml2YXIgNC4wIEludGVybmFjaW9uYWwuCg==
dc.title.es_PE.fl_str_mv Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
title Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
spellingShingle Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
Álvarez, Félix
Extracción de adn
Mutantes inducidos por radiación
Algodón
title_short Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
title_full Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
title_fullStr Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
title_full_unstemmed Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
title_sort Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
dc.creator.none.fl_str_mv Leyva, Omar
Álvarez, Félix
Osores, José
author Álvarez, Félix
author_facet Álvarez, Félix
Leyva, Omar
Osores, José
author_role author
author2 Leyva, Omar
Osores, José
author2_role author
author
dc.contributor.author.fl_str_mv Álvarez, Félix
Leyva, Omar
Osores, José
dc.subject.es_PE.fl_str_mv Extracción de adn
Mutantes inducidos por radiación
Algodón
topic Extracción de adn
Mutantes inducidos por radiación
Algodón
description A fast, simple and reliable method for the extraction of DNA from Gossypium barbadense radioinduced mutants has been developed. This method is cetyltrimethylammonium bromide (cTAB) based, and it extracts DNA from seed leaves processed in a 1.5 mL tube with 0.7 mL of extraction buffer; is highly efficient to cotton tissue and much cheaper in terms of time, chemical use and labor input. The described protocol presents consistent yield and good quality of DNA for several molecular analyses (AFLP, SSR, etc.).
publishDate 2008
dc.date.accessioned.none.fl_str_mv 2016-07-18T17:22:16Z
dc.date.available.none.fl_str_mv 2016-07-18T17:22:16Z
dc.date.issued.fl_str_mv 2008-11
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/workingPaper
format workingPaper
dc.identifier.citation.es_PE.fl_str_mv Álvarez F, Leyva O, Osores J. Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies. Informe Científico Tecnológico. 2007; 7: 183-186.
dc.identifier.issn.none.fl_str_mv 1684-1662
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.13054/562
identifier_str_mv Álvarez F, Leyva O, Osores J. Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies. Informe Científico Tecnológico. 2007; 7: 183-186.
1684-1662
url https://hdl.handle.net/20.500.13054/562
dc.language.iso.es_PE.fl_str_mv eng
language eng
dc.rights.es_PE.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.es_PE.fl_str_mv application/pdf
dc.publisher.es_PE.fl_str_mv Lima (Perú)
dc.publisher.country.es_PE.fl_str_mv PE
dc.source.es_PE.fl_str_mv Instituto Peruano de Energía Nuclear
Repositorio Institucional del Instituto Peruano de Energía Nuclear
dc.source.none.fl_str_mv reponame:IPEN-Institucional
instname:Instituto Peruano de Energía Nuclear
instacron:IPEN
instname_str Instituto Peruano de Energía Nuclear
instacron_str IPEN
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reponame_str IPEN-Institucional
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Genomic DNA extraction from seed leaves of Gossypium barbadense L. for radioinduced mutations studies
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