Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru

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This study was funded by a grant from the National Fund for Scientific, Technological Development and Technological Innovation (FONDECYT) of the National Council of Science, Technology and Technological Innovation (CONCYTEC) of Peru, Contract N° 171-2015-FONDECYT.
Detalles Bibliográficos
Autores: Coronado-Olano J., Repo-Carrasco-Valencia R., Reategui O., Toscano E., Valdez E., Zimic M., Best I.
Formato: artículo
Fecha de Publicación:2021
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/3013
Enlace del recurso:https://hdl.handle.net/20.500.12390/3013
https://doi.org/10.5530/pj.2021.13.115
Nivel de acceso:acceso abierto
Materia:Polyphenols
Andean grains
DPPH radical scavenging assay
HPLC-DAD
In vitro antidiabetic activity
https://purl.org/pe-repo/ocde/ford#3.01.03
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oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/3013
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
title Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
spellingShingle Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
Coronado-Olano J.
Polyphenols
Andean grains
Andean grains
DPPH radical scavenging assay
DPPH radical scavenging assay
HPLC-DAD
In vitro antidiabetic activity
In vitro antidiabetic activity
https://purl.org/pe-repo/ocde/ford#3.01.03
title_short Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
title_full Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
title_fullStr Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
title_full_unstemmed Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
title_sort Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
author Coronado-Olano J.
author_facet Coronado-Olano J.
Repo-Carrasco-Valencia R.
Reategui O.
Toscano E.
Valdez E.
Zimic M.
Best I.
author_role author
author2 Repo-Carrasco-Valencia R.
Reategui O.
Toscano E.
Valdez E.
Zimic M.
Best I.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coronado-Olano J.
Repo-Carrasco-Valencia R.
Reategui O.
Toscano E.
Valdez E.
Zimic M.
Best I.
dc.subject.none.fl_str_mv Polyphenols
topic Polyphenols
Andean grains
Andean grains
DPPH radical scavenging assay
DPPH radical scavenging assay
HPLC-DAD
In vitro antidiabetic activity
In vitro antidiabetic activity
https://purl.org/pe-repo/ocde/ford#3.01.03
dc.subject.es_PE.fl_str_mv Andean grains
Andean grains
DPPH radical scavenging assay
DPPH radical scavenging assay
HPLC-DAD
In vitro antidiabetic activity
In vitro antidiabetic activity
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#3.01.03
description This study was funded by a grant from the National Fund for Scientific, Technological Development and Technological Innovation (FONDECYT) of the National Council of Science, Technology and Technological Innovation (CONCYTEC) of Peru, Contract N° 171-2015-FONDECYT.
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/3013
dc.identifier.doi.none.fl_str_mv https://doi.org/10.5530/pj.2021.13.115
dc.identifier.scopus.none.fl_str_mv 2-s2.0-85110944883
url https://hdl.handle.net/20.500.12390/3013
https://doi.org/10.5530/pj.2021.13.115
identifier_str_mv 2-s2.0-85110944883
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Pharmacognosy Journal
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.publisher.none.fl_str_mv EManuscript Technologies
publisher.none.fl_str_mv EManuscript Technologies
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
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spelling Publicationrp08620600rp01728600rp08621600rp08622600rp08623600rp00606600rp06463600Coronado-Olano J.Repo-Carrasco-Valencia R.Reategui O.Toscano E.Valdez E.Zimic M.Best I.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2021https://hdl.handle.net/20.500.12390/3013https://doi.org/10.5530/pj.2021.13.1152-s2.0-85110944883This study was funded by a grant from the National Fund for Scientific, Technological Development and Technological Innovation (FONDECYT) of the National Council of Science, Technology and Technological Innovation (CONCYTEC) of Peru, Contract N° 171-2015-FONDECYT.Background: Chenopodium quinoa Willd. and Chenopodium pallidicaule Aellen are grains from the Andean region of Peru, which in addition to having a high nutritional value, have health-promoting properties related to the prevention of chronic diseases such as diabetes. Objetive: The present study aimed to identify phenolic compounds associated with an inhibition of carbohydrate hydrolyzing enzymes associated with type 2 diabetes. Material and Methods: Two varieties of quinoa and two varieties of cañihua from the Puno Region in Peru, were evaluated. Total phenolics, total flavonoids, identification of phenolic compounds and antioxidant activity in vitro were measured by Folin-Ciocalteu assay, aluminum chloride colorimetric method, HPLC-DAD and DPPH radical scavenging assay, respectively. In vitro hypoglycemic activity was evaluated through the inhibition of the ?-amylase and ?-glucosidase enzymes. Results: Gallic acid, rutin and chlorogenic acid were identified by HPLC-DAD in the varieties of quinoa and cañihua. The latter showed significantly higher levels of chlorogenic acid compared to quinoa varieties (p&lt;0.05). Both Andean grains exhibited inhibition of key-enzymes linked to type 2 diabetes, presenting IC50 values of 7.99 to 34.05 and of 8.07 to 1158 µg/mL for ?-amylase and ?-glucosidase, respectively. Total phenolics, total flavonoids, DPPH radical scavenging assay, gallic acid and chlorogenic acid showed the greatest contribution to the inhibitory activity of the ?-glucosidase enzyme (p&lt;0.05). Conclusion: Our findings suggest that the phenolic compounds present in the varieties of quinoa and cañihua could modulate the inhibition of carbohydrate hydrolyzing enzymes associated with type 2 diabetes. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengEManuscript TechnologiesPharmacognosy Journalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/PolyphenolsAndean grains-1Andean grains-1DPPH radical scavenging assay-1DPPH radical scavenging assay-1HPLC-DAD-1In vitro antidiabetic activity-1In vitro antidiabetic activity-1https://purl.org/pe-repo/ocde/ford#3.01.03-1Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peruinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/3013oai:repositorio.concytec.gob.pe:20.500.12390/30132024-05-30 16:13:08.846https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="26eb3c57-c2e4-46f5-9072-ed8ae02fb1ec"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru</Title> <PublishedIn> <Publication> <Title>Pharmacognosy Journal</Title> </Publication> </PublishedIn> <PublicationDate>2021</PublicationDate> <DOI>https://doi.org/10.5530/pj.2021.13.115</DOI> <SCP-Number>2-s2.0-85110944883</SCP-Number> <Authors> <Author> <DisplayName>Coronado-Olano J.</DisplayName> <Person id="rp08620" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Repo-Carrasco-Valencia R.</DisplayName> <Person id="rp01728" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Reategui O.</DisplayName> <Person id="rp08621" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Toscano E.</DisplayName> <Person id="rp08622" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Valdez E.</DisplayName> <Person id="rp08623" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Zimic M.</DisplayName> <Person id="rp00606" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Best I.</DisplayName> <Person id="rp06463" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>EManuscript Technologies</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>Polyphenols</Keyword> <Keyword>Andean grains</Keyword> <Keyword>Andean grains</Keyword> <Keyword>DPPH radical scavenging assay</Keyword> <Keyword>DPPH radical scavenging assay</Keyword> <Keyword>HPLC-DAD</Keyword> <Keyword>In vitro antidiabetic activity</Keyword> <Keyword>In vitro antidiabetic activity</Keyword> <Abstract>Background: Chenopodium quinoa Willd. and Chenopodium pallidicaule Aellen are grains from the Andean region of Peru, which in addition to having a high nutritional value, have health-promoting properties related to the prevention of chronic diseases such as diabetes. Objetive: The present study aimed to identify phenolic compounds associated with an inhibition of carbohydrate hydrolyzing enzymes associated with type 2 diabetes. Material and Methods: Two varieties of quinoa and two varieties of cañihua from the Puno Region in Peru, were evaluated. Total phenolics, total flavonoids, identification of phenolic compounds and antioxidant activity in vitro were measured by Folin-Ciocalteu assay, aluminum chloride colorimetric method, HPLC-DAD and DPPH radical scavenging assay, respectively. In vitro hypoglycemic activity was evaluated through the inhibition of the ?-amylase and ?-glucosidase enzymes. Results: Gallic acid, rutin and chlorogenic acid were identified by HPLC-DAD in the varieties of quinoa and cañihua. The latter showed significantly higher levels of chlorogenic acid compared to quinoa varieties (p&amp;lt;0.05). Both Andean grains exhibited inhibition of key-enzymes linked to type 2 diabetes, presenting IC50 values of 7.99 to 34.05 and of 8.07 to 1158 µg/mL for ?-amylase and ?-glucosidase, respectively. Total phenolics, total flavonoids, DPPH radical scavenging assay, gallic acid and chlorogenic acid showed the greatest contribution to the inhibitory activity of the ?-glucosidase enzyme (p&amp;lt;0.05). Conclusion: Our findings suggest that the phenolic compounds present in the varieties of quinoa and cañihua could modulate the inhibition of carbohydrate hydrolyzing enzymes associated with type 2 diabetes. © 2021 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
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Inhibitory activity against ?-amylase and ?-glucosidase by phenolic compounds of quinoa (Chenopodium quinoa Willd.) and cañihua (Chenopodium pallidicaule Aellen) from the Andean region of Peru
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