Ameliorative Effect of the Oral Administration of Chuquiraga spinosa in a Murine Model of Breast Cancer Induced with 7,12-Dimethylbenz[a]anthracene (DMBA)

• Autores: Arroyo-Acevedo, Jorge Luis, Herrera-Calderon, Oscar, Tinco-Jayo, Johnny Aldo, Rojas-Armas, Juan Pedro, Rauf, Abdur, Hañari-Quispe, Renán, Figueroa-Salvador, Linder, Fernández-Guzmán, Victor, Yuli-Posadas, Ricardo Ángel
• Formato: artículo
• Fecha de Publicación: 2020
• Institución: Universidad Peruana de Ciencias Aplicadas
• Repositorio: UPC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorioacademico.upc.edu.pe:10757/655585
• Enlace del recurso: https://doi.org/10.5530/pj.2020.12.85; http://hdl.handle.net/10757/655585
• Nivel de acceso: acceso abierto
• Materia: Anticarcinogenic agent; Antioxidant; Breast tumor; Phytochemical; Preventive medicine; Toxicity; 7,12 dimethylbenz[a]anthracene; Alanine aminotransferasea; Alkaline phosphatase; Alkaloid derivative; https://purl.org/pe-repo/ocde/ford#3.00.00

Objective: To determine the ameliorative effect of the ethanolic extract of Chuquiraga spinosa (ChS) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced breast cancer in rats. Methods: 36 female Holztman rats were divided into 6 groups. I) The negative control group received physiological saline (PS). II) ChS-200 group received 200 mg/kg of ChS. III) DMBA group was induced with DMBA (20 mg/Kg) dissolved in PS and administrated orally for 15 weeks. IV) DMBA + ChS-50 group, V) DMBA + ChS-250 group, and VI) DMBA + ChS-500 group, which received the extract orally for 15 weeks after DMBA induction. All data were expressed as mean and standard deviation. One-way analysis of variance (ANOVA) followed by Dunnet test was carried out to compare the mean value of different groups Histopathological analysis was evaluated by using Image J software. Results: Hematology showed that the triglyceride level was significantly lowered (P< 0.01) and high-density lipoprotein (HDL) level was significantly increased (P <0.01) in groups III, IV and V. Also, ChS extract significantly lowered the C reactive protein (CRP) level (P <0.01) and malondialdehyde level (P<0.05). There was a significant decrease in the frequency of DMBA-induced micronucleated polychromatic erythrocyte (P<0.01). Conclusions: Chuquiraga spinosa showed an ameliorative effect on DMBA-induced breast cancer in rats as well as antioxidant, antitumor and antigenotoxic properties.