Diagnostic performance of a point-of-care molecular system for the detection of sars-cov-2 in Peru

• Autores: Puyén, Zully M., Araujo-Castillo, Roger, Giraldo, Jorge, Gutierrez, Karina, Rojas-Serrano, Nancy
• Formato: artículo
• Fecha de Publicación: 2024
• Institución: Universidad Peruana de Ciencias Aplicadas
• Repositorio: UPC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorioacademico.upc.edu.pe:10757/675841
• Enlace del recurso: https://doi.org/10.17843/rpmesp.2024.411.13046; http://hdl.handle.net/10757/675841
• Nivel de acceso: acceso abierto
• Materia: COVID-19; Molecular Diagnostic Techniques; PCR; Peru (source: MeSH NLM); SARS-CoV-2; Sensitivity and Specificity; https://purl.org/pe-repo/ocde/ford#3.00.00

The present study assessed the diagnostic performance of the Xpert®Xpress SARS-CoV-2 test in comparison with the Charité protocol real-time RT PCR for the detection of SARS-CoV-2 in Peruvian patients. This was a diagnostic test study that included 100 nasal and pharyngeal swab samples. We obtained an overall concordance of 98.70% (95%CI: 92.98-99.97), with a kappa coefficient of 0.97 (95%CI: 0.86-1.00) and sensitivity and relative specificity rates of 100% and 96.15%, respectively. Additionally, the percentage of the area under the ROC curve was 98.08% in both cases, and an analytical specificity rate of 100% was obtained for the different respiratory viruses evaluated. In conclusion, the Xpert®Xpress SARS-CoV-2 test, by using nasal and pharyngeal swab samples, was highly sensitive and specific, and the kappa coefficient showed an excellent correlation when compared to the reference test