Development and analysis of furazolidone-resistant Escherichia coli mutants
Descripción del Articulo
Revisión por pares
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2015 |
| Institución: | Universidad Peruana de Ciencias Aplicadas |
| Repositorio: | UPC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorioacademico.upc.edu.pe:10757/556955 |
| Enlace del recurso: | http://hdl.handle.net/10757/556955 |
| Nivel de acceso: | acceso abierto |
| Materia: | Enterobacteriaceae Nitrofuran Resistance mechanisms |
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| dc.title.es_PE.fl_str_mv |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| title |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| spellingShingle |
Development and analysis of furazolidone-resistant Escherichia coli mutants Martínez Puchol, Sandra Enterobacteriaceae Nitrofuran Resistance mechanisms |
| title_short |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| title_full |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| title_fullStr |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| title_full_unstemmed |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| title_sort |
Development and analysis of furazolidone-resistant Escherichia coli mutants |
| author |
Martínez Puchol, Sandra |
| author_facet |
Martínez Puchol, Sandra Gómes, Cláudia Pons, Maria J. Ruíz Roldan, Lidia Torrents De La Peña, Alba Ochoa, Theresa J. Ruíz, Joaquim |
| author_role |
author |
| author2 |
Gómes, Cláudia Pons, Maria J. Ruíz Roldan, Lidia Torrents De La Peña, Alba Ochoa, Theresa J. Ruíz, Joaquim |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Martínez Puchol, Sandra Gómes, Cláudia Pons, Maria J. Ruíz Roldan, Lidia Torrents De La Peña, Alba Ochoa, Theresa J. Ruíz, Joaquim |
| dc.subject.es_PE.fl_str_mv |
Enterobacteriaceae Nitrofuran Resistance mechanisms |
| topic |
Enterobacteriaceae Nitrofuran Resistance mechanisms |
| description |
Revisión por pares |
| publishDate |
2015 |
| dc.date.accessioned.es_PE.fl_str_mv |
2015-06-15T16:20:48Z |
| dc.date.available.es_PE.fl_str_mv |
2015-06-15T16:20:48Z |
| dc.date.issued.fl_str_mv |
2015-06-15 |
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info:eu-repo/semantics/article |
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article |
| dc.identifier.citation.es_PE.fl_str_mv |
Mart ınez-Puchol S, Gomes C, Pons MJ, Ruiz-Rold an L, Torrents de la Pe~na A, Ochoa TJ, Ruiz J. Development and analysis of furazolidone-resistant Escherichia coli mutants. APMIS 2015. |
| dc.identifier.issn.es_PE.fl_str_mv |
1600-0463 |
| dc.identifier.pmid.es_PE.fl_str_mv |
26011027 |
| dc.identifier.doi.es_PE.fl_str_mv |
10.1111/apm.12401 |
| dc.identifier.uri.es_PE.fl_str_mv |
http://hdl.handle.net/10757/556955 |
| dc.identifier.journal.es_PE.fl_str_mv |
Acta pathologica, microbiologica et Immunológica Scandinavica (APMIS.) |
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Mart ınez-Puchol S, Gomes C, Pons MJ, Ruiz-Rold an L, Torrents de la Pe~na A, Ochoa TJ, Ruiz J. Development and analysis of furazolidone-resistant Escherichia coli mutants. APMIS 2015. 1600-0463 26011027 10.1111/apm.12401 Acta pathologica, microbiologica et Immunológica Scandinavica (APMIS.) |
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http://hdl.handle.net/10757/556955 |
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eng |
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eng |
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http://www.ncbi.nlm.nih.gov/pubmed/26011027 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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John Wiley & Sons, Inc. |
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Universidad Peruana de Ciencias Aplicadas (UPC) Repositorio Académico - UPC |
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Martínez Puchol, SandraGómes, CláudiaPons, Maria J.Ruíz Roldan, LidiaTorrents De La Peña, AlbaOchoa, Theresa J.Ruíz, Joaquim2015-06-15T16:20:48Z2015-06-15T16:20:48Z2015-06-15Mart ınez-Puchol S, Gomes C, Pons MJ, Ruiz-Rold an L, Torrents de la Pe~na A, Ochoa TJ, Ruiz J. Development and analysis of furazolidone-resistant Escherichia coli mutants. APMIS 2015.1600-04632601102710.1111/apm.12401http://hdl.handle.net/10757/556955Acta pathologica, microbiologica et Immunológica Scandinavica (APMIS.)Revisión por paresjoruiz@clinic.ub.esFurazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe-Arg-β-Naphtylamyde. The presence of mutations in the nitroreductase genes nfsA and nfsB was analysed by PCR; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by SDS-PAGE. The frequency of mutation ranged from <9.6 × 10-10 to 9.59 × 10-7 . Neither an effect on efflux pumps inhibited by Phe-Arg-β-Naphtylamyde nor cross-resistance with the antibiotics studied was observed. Nineteen mutants (52.94%) presented mutations in the nitroreductase-encoding genes: 17 in the nfsA gene (15 mutants with an internal stop codon, 2 with amino acid changes), 2 in the nfsB (all amino acid changes). Alterations in the outer membrane proteins OmpA and OmpW were also observed. Although more studies are necessary to find other resistance mechanisms, present data showed the low potential of selecting furazolidone-resistant mutants, together with the lack of cross-resistance with unrelated antimicrobial agents.This work was supported by the Agencia Espa~nola de Cooperaci on Internacional al Desarrollo (AECID) [grants nos. D/024648/09 and D/030509/10] and by Generalitat de Catalunya, Departament d’Universitats, Recerca i Societat de la Informaci o (2014 SGR 26). JR has a fellowship from the programme I3 of the Instituto de Salud Carlos III (ISCIII, Spain) [grant no. CES11/012].application/pdfengJohn Wiley & Sons, Inc.http://www.ncbi.nlm.nih.gov/pubmed/26011027info:eu-repo/semantics/openAccessUniversidad Peruana de Ciencias Aplicadas (UPC)Repositorio Académico - UPCreponame:UPC-Institucionalinstname:Universidad Peruana de Ciencias Aplicadasinstacron:UPCEnterobacteriaceaeddc1bdb9-7be2-423f-b513-bb24d4a6bb5a600Nitrofuran2ad2ee0c-1c94-402d-9322-9ba2b667b2bb600Resistance mechanisms79de3a44-9648-4266-9371-febebfa6d67c600Development and analysis of furazolidone-resistant Escherichia coli mutantsinfo:eu-repo/semantics/article2018-06-23T01:49:11ZFurazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe-Arg-β-Naphtylamyde. The presence of mutations in the nitroreductase genes nfsA and nfsB was analysed by PCR; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by SDS-PAGE. The frequency of mutation ranged from <9.6 × 10-10 to 9.59 × 10-7 . Neither an effect on efflux pumps inhibited by Phe-Arg-β-Naphtylamyde nor cross-resistance with the antibiotics studied was observed. Nineteen mutants (52.94%) presented mutations in the nitroreductase-encoding genes: 17 in the nfsA gene (15 mutants with an internal stop codon, 2 with amino acid changes), 2 in the nfsB (all amino acid changes). Alterations in the outer membrane proteins OmpA and OmpW were also observed. Although more studies are necessary to find other resistance mechanisms, present data showed the low potential of selecting furazolidone-resistant mutants, together with the lack of cross-resistance with unrelated antimicrobial agents.ORIGINALEscherichia coli mutants.pdfEscherichia coli mutants.pdfapplication/pdf156705https://repositorioacademico.upc.edu.pe/bitstream/10757/556955/1/Escherichia%20coli%20mutants.pdf8af7e940105d753c9dce7b33da1f4156MD51trueLICENSElicense.txtlicense.txttext/plain; charset=utf-81659https://repositorioacademico.upc.edu.pe/bitstream/10757/556955/2/license.txt1ed8f33c5404431ad7aabc05080746c5MD52falseTEXTEscherichia coli mutants.pdf.txtEscherichia coli mutants.pdf.txtExtracted Texttext/plain27242https://repositorioacademico.upc.edu.pe/bitstream/10757/556955/3/Escherichia%20coli%20mutants.pdf.txt268721602583340fd7d2deab672246dbMD53falseTHUMBNAILEscherichia coli mutants.pdf.jpgEscherichia coli mutants.pdf.jpgGenerated Thumbnailimage/jpeg119133https://repositorioacademico.upc.edu.pe/bitstream/10757/556955/4/Escherichia%20coli%20mutants.pdf.jpg503c5e9adeb14fb9d9f1faaf001ce8cbMD54false10757/556955oai:repositorioacademico.upc.edu.pe:10757/5569552019-08-30 08:01:08.24Repositorio académico upcupc@openrepository.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 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
