3D hanging spheroid plate for high-throughput CAR T cell cytotoxicity assay

• Autores: Chen, Zhenzhong, Han, Seokgyu, Sanny, Arleen, Leung-Kwan Chan, Dorothy, Noort, Danny van, Lim, Wanyoung, Hee-Meng Tan, Andy, Park, Sungsu
• Formato: artículo
• Fecha de Publicación: 2022
• Institución: Universidad de Ingeniería y tecnología
• Repositorio: UTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.utec.edu.pe:20.500.12815/553
• Enlace del recurso: https://hdl.handle.net/20.500.12815/553; https://doi.org/10.1186/s12951-021-01213-8
• Nivel de acceso: acceso abierto
• Materia: HER2-CAR T cell; 3D hanging spheroid plate; Tumor spheroid; Cytotoxicity assay; https://purl.org/pe-repo/ocde/ford#2.06.01

Most high-throughput screening (HTS) systems studying the cytotoxic effect of chimeric antigen receptor (CAR) T cells on tumor cells rely on two-dimensional cell culture that does not recapitulate the tumor microenvironment (TME). Tumor spheroids, however, can recapitulate the TME and have been used for cytotoxicity assays of CAR T cells. But a major obstacle to the use of tumor spheroids for cytotoxicity assays is the difficulty in separating unbound CAR T and dead tumor cells from spheroids. Here, we present a three-dimensional hanging spheroid plate (3DHSP), which facilitates the formation of spheroids and the separation of unbound and dead cells from spheroids during cytotoxicity assays.