Distribution of tumor-infiltrating immune cells in glioblastoma

• Autor: Castaneda, Carlos A.
• Formato: artículo
• Fecha de Publicación: 2018
• Institución: Universidad Privada San Juan Bautista
• Repositorio: UPSJB-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.upsjb.edu.pe:upsjb/2646
• Enlace del recurso: http://repositorio.upsjb.edu.pe/handle/upsjb/2646
• Nivel de acceso: acceso abierto
• Materia: Biomarcador; Glioblastoma; Macrófagos; MGMT; Supervivencia general; Pronóstico; Infiltración tumoral linfocitos

Aim: Evaluation of features related to infiltrating immune cell level in glioblastoma. Methods: Tumor- infiltrating lymphocytes (TILs) through H&E staining, and TILs (CD3, CD4, CD8 and CD20) and macrophage (CD68 and CD163) levels through immunohistochemistry were evaluated through digital analysis. Results: CD68 (9.1%), CD163 (2.2%), CD3 (1.6%) and CD8 (1.6%) had the highest density. Higher CD4 + was as- sociated with unmethylated MGMT (p = 0.016). Higher CD8 + was associated with larger tumoral size (p = 0.027). Higher CD163 + was associated with higher age (p = 0.044) and recursive partitioning anal- ysis = 4. Women (p < 0.05), total resection (p < 0.05), MGMT-methylation (p < 0.001), radiotherapy (p < 0.001), chemotherapy (p < 0.001) and lower CD4 + (p < 0.05) were associated with longer overall survival. Conclusion: Macrophages are more frequent than TILs. Some subsets are associated with clinical features.