Caracterización molecular de la resistencia antimicrobiana de Helicobacter pylori en pacientes dispépticos del Hospital y la Clínica Médica Cayetano Heredia

• Autor: Chu Mendoza, Manuel Enrique
• Formato: tesis de maestría
• Fecha de Publicación: 2021
• Institución: Universidad Peruana Cayetano Heredia
• Repositorio: UPCH-Institucional
• Lenguaje: español
• OAI Identifier: oai:repositorio.upch.edu.pe:20.500.12866/8992
• Enlace del recurso: https://hdl.handle.net/20.500.12866/8992
• Nivel de acceso: acceso abierto
• Materia: Helicobacter pylori; Resistencia Antimicrobiana; Mutaciones Genéticas; Pacientes Dispépticos; https://purl.org/pe-repo/ocde/ford#1.06.03; https://purl.org/pe-repo/ocde/ford#3.02.19; https://purl.org/pe-repo/ocde/ford#3.03.08

The present research work is a descriptive and cross-sectional study, in which 1500 gastric biopsies collected from 500 dyspeptic patients were processed with the aim of isolating and cultivating H. pylori strains, determining the resistance profiles in vitro and identify genetic mutations in the rRNA23S, pbp1, gyrA, rdxA and rRNA16S genes to determine the statistical significance and prevalence ratios between antimicrobial resistance to Clarithromycin, Amoxicillin, Levofloxacin, Metronidazole and Tetracycline respectively. As results, a prevalence of 54.6% of dyspeptic patients with H. pylori was obtained with antimicrobial resistance of: Clarithromycin 35.2%, Amoxicillin 65.3%, Metronidazole 68.9%, Levofloxacin 72.1% and Tetracycline 7.8%. In addition, mutations A2143G, A2142G and A2142C are identified within the rRNA23S and N87I, D91G, N87K, D91N and D91Y genes within the gyrA gene, significantly related to resistance to Clarithromycin and Levofloxacin respectively and a high genetic diversity in the genes pbp1 and rdxA, with mutations that do not show a significant relationship with high resistance to Amoxicillin and Metronidazole respectively. Finally, the presence of mutations in positions 926-928 of the rRNA16S gene is reported, without presenting a significant relationship with low resistance to Tetracycline.