Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis

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Introduction. The Toll-like receptor (TLR) interacting with the promastigote of Leishmania spp. determines the cellular activation pathway. Objective. To determine the transcriptional expression of TLR-3, TLR-4, TLR-9, IL-12 and TNF-α in macrophages infected with a native strain of L. braziliensis (...

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Detalles Bibliográficos
Autores: Torres Gonzales, Dina, Alzamora-Gonzales, Libertad
Formato: artículo
Fecha de Publicación:2024
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/26800
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/26800
Nivel de acceso:acceso abierto
Materia:Leishmania braziliensis
Citocinas
Fagocitosis
Óxido Nítrico
Leishmaniasis Cutánea
Cytokines
Phagocytosis
Nitric Oxide
Cutaneous leishmaniasis
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spelling Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensisExpresión transcripcional de receptores tipo Toll y citoquinas proinflamatorias en macrófagos infectados con Leishmania braziliensis nativaTorres Gonzales, DinaAlzamora-Gonzales, LibertadTorres Gonzales, DinaAlzamora-Gonzales, LibertadLeishmania braziliensisCitocinasFagocitosisÓxido NítricoLeishmaniasis CutáneaLeishmania braziliensisCytokinesPhagocytosisNitric OxideCutaneous leishmaniasisIntroduction. The Toll-like receptor (TLR) interacting with the promastigote of Leishmania spp. determines the cellular activation pathway. Objective. To determine the transcriptional expression of TLR-3, TLR-4, TLR-9, IL-12 and TNF-α in macrophages infected with a native strain of L. braziliensis (Lbn). Materials and Methods. Identification of Lbn was performed by qPCR for kinetoplast DNA sequences. Mouse peritoneal macrophages were infected with promastigotes (MI) and nitric oxide (NO) production was measured; transcript levels for TLRs and cytokines were quantified by qRT-PCR. Results. Lbn showed 96% homology to L. braziliensis. High ON production was observed in IMs at 2 h; significant transcriptional expression especially of TLR-3 and TLR-9, which corresponded with expression for cytokines. Conclusions. Lbn strongly activated macrophages via endosomal TLRs, which can be applied in the design of agonists to treat the disease.Introducción. El receptor de tipo Toll (TLR) que interactúe con el promastigote de Leishmania spp.determina la vía de activación celular. Objetivo. Identificar la expresión transcripcional de TLR-3, TLR-4, TLR-9, IL-12 y TNF-α en macrófagos infectados con una cepa nativa de L. braziliensis (Lbn). Métodos. La identificación de Lbn se hizo empleando qPCR para secuencias del DNA del cinetoplasto. Los macrófagos peritoneales de ratones fueron infectados con promastigotes y se midieron la producción de óxido nítrico (ON). Se cuantificaron los niveles transcripcionales para TLRs y citoquinas empleando qRT-PCR. Resultados. Lbn presentó 96% de homología con L. braziliensis. En los infectados con promastigotes se observó elevada producción de ON a las 2 h; significativa expresión transcripcional especialmente de TLR-3 y TLR-9 que se correspondió con la expresión para citoquinas. Conclusión. Lbn activó fuertemente a los macrófagos mediante los TLRs endosomales lo cual puede ser aplicado en el diseño de agonistas para tratar la enfermedad.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Humana2024-03-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/2680010.15381/anales.v85i1.26800Anales de la Facultad de Medicina; Vol. 85 No. 1 (2024); 51-56Anales de la Facultad de Medicina; Vol. 85 Núm. 1 (2024); 51-561609-94191025-5583reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/26800/20760Derechos de autor 2024 Anales de la Facultad de Medicinahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/268002024-03-28T01:59:07Z
dc.title.none.fl_str_mv Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
Expresión transcripcional de receptores tipo Toll y citoquinas proinflamatorias en macrófagos infectados con Leishmania braziliensis nativa
title Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
spellingShingle Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
Torres Gonzales, Dina
Leishmania braziliensis
Citocinas
Fagocitosis
Óxido Nítrico
Leishmaniasis Cutánea
Leishmania braziliensis
Cytokines
Phagocytosis
Nitric Oxide
Cutaneous leishmaniasis
title_short Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
title_full Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
title_fullStr Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
title_full_unstemmed Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
title_sort Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
dc.creator.none.fl_str_mv Torres Gonzales, Dina
Alzamora-Gonzales, Libertad
Torres Gonzales, Dina
Alzamora-Gonzales, Libertad
author Torres Gonzales, Dina
author_facet Torres Gonzales, Dina
Alzamora-Gonzales, Libertad
author_role author
author2 Alzamora-Gonzales, Libertad
author2_role author
dc.subject.none.fl_str_mv Leishmania braziliensis
Citocinas
Fagocitosis
Óxido Nítrico
Leishmaniasis Cutánea
Leishmania braziliensis
Cytokines
Phagocytosis
Nitric Oxide
Cutaneous leishmaniasis
topic Leishmania braziliensis
Citocinas
Fagocitosis
Óxido Nítrico
Leishmaniasis Cutánea
Leishmania braziliensis
Cytokines
Phagocytosis
Nitric Oxide
Cutaneous leishmaniasis
description Introduction. The Toll-like receptor (TLR) interacting with the promastigote of Leishmania spp. determines the cellular activation pathway. Objective. To determine the transcriptional expression of TLR-3, TLR-4, TLR-9, IL-12 and TNF-α in macrophages infected with a native strain of L. braziliensis (Lbn). Materials and Methods. Identification of Lbn was performed by qPCR for kinetoplast DNA sequences. Mouse peritoneal macrophages were infected with promastigotes (MI) and nitric oxide (NO) production was measured; transcript levels for TLRs and cytokines were quantified by qRT-PCR. Results. Lbn showed 96% homology to L. braziliensis. High ON production was observed in IMs at 2 h; significant transcriptional expression especially of TLR-3 and TLR-9, which corresponded with expression for cytokines. Conclusions. Lbn strongly activated macrophages via endosomal TLRs, which can be applied in the design of agonists to treat the disease.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/26800
10.15381/anales.v85i1.26800
url https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/26800
identifier_str_mv 10.15381/anales.v85i1.26800
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/26800/20760
dc.rights.none.fl_str_mv Derechos de autor 2024 Anales de la Facultad de Medicina
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2024 Anales de la Facultad de Medicina
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Humana
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Humana
dc.source.none.fl_str_mv Anales de la Facultad de Medicina; Vol. 85 No. 1 (2024); 51-56
Anales de la Facultad de Medicina; Vol. 85 Núm. 1 (2024); 51-56
1609-9419
1025-5583
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.959956
Transcriptional expression of Toll-like receptors and proinflammatory cytokines in macrophages infected with native Leishmania braziliensis
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