Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens

Descripción del Articulo

The study aimed to identify Staphylococcus spp with phenotypes and genotypes of methicillin resistance, as well as to identify variants of the coa + gene polymorphism in S. aureus. In total, 97 strains of Staphylococcus spp isolated from chickens were used and antibiograms and multiplex PCR, convent...

Descripción completa

Detalles Bibliográficos
Autores: Cotaquispe, Rony, Sarmiento, Ruth, Lovón, Stephane, Rodriguez, Jorge
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/20395
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20395
Nivel de acceso:acceso abierto
Materia:methicillin-resistant Staphylococcus aureus
methicillin-resistant coagulase positive staphylococci
resistance genes
Staphylococcus aureus resistente a la meticilina
estafilococos coagulasa positivos a la meticilina resistente
genes de resistencia
id REVUNMSM_d1930997a06c4284a416af6e43a07585
oai_identifier_str oai:ojs.csi.unmsm:article/20395
network_acronym_str REVUNMSM
network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
spelling Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickensCaracterización fenotípica y genotípica de Staphylococcus spp con resistencia a meticilina en pollos comercialesCotaquispe, RonySarmiento, RuthLovón, StephaneRodriguez, JorgeCotaquispe, RonySarmiento, RuthLovón, StephaneRodriguez, Jorgemethicillin-resistant Staphylococcus aureusmethicillin-resistant coagulase positive staphylococciresistance genesStaphylococcus aureus resistente a la meticilinaestafilococos coagulasa positivos a la meticilina resistentegenes de resistenciaThe study aimed to identify Staphylococcus spp with phenotypes and genotypes of methicillin resistance, as well as to identify variants of the coa + gene polymorphism in S. aureus. In total, 97 strains of Staphylococcus spp isolated from chickens were used and antibiograms and multiplex PCR, conventional PCR and sequencing tests were carried out. The phenotypic results indicate the presence of resistance to 2 MRSA and 26 SCN resistant to methicillin (cefoxitin) and 2 S. aureus and 67 SCN with presumed resistance to methicillin (oxacillin) in the antibiograms. Using PCR, the mecA gene responsible for methicillin resistance was identified in 24 strains: 2 MRSA and 22 SCNRM. In addition, 4 S. aureus genotypes with variants of the coagulase gene polymorphism were identified. The results suggest a constant genetic activity, the appearance of clones and the expression of genes for resistance to the main antibiotics faced by Staphylococcus spp.El estudio tuvo como objetivo identificar Staphylococcus spp con fenotipos y genotipos de resistencia a meticilina, así como identificar variantes del polimorfismo del gen coa + en S. aureus. Se utilizaron 97 cepas de Staphylococcus spp aislados de pollos y se hicieron antibiogramas y pruebas de PCR multiplex, PCR convencional y secuenciamiento. Los resultados fenotípicos indican la presencia de resistencia a 2 SARM y 26 SCN resistentes a meticilina (cefoxitina) y 2 S. aureus y 67 SCN con presunta resistencia a meticilina (oxacilina) en las pruebas de antibiograma. Mediante PCR se identificó el gen mecA responsable de la resistencia a meticilina en 24 cepas: 2 SARM y 22 SCNRM y se identificaron cuatro genotipos de S. aureus con variantes del polimorfismo del gen coagulasa. Los resultados sugieren una actividad genética constante, la aparición de clones y la expresión de genes para la resistencia a los principales antibióticos que enfrentan Staphylococcus spp.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2021-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2039510.15381/rivep.v32i3.20395Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 3 (2021); e20395Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 3 (2021); e203951682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20395/16825Derechos de autor 2021 Rony Cotaquispe, Ruth Sarmiento, Stephane Lovón, Jorge Rodriguezhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/203952021-06-25T11:18:37Z
dc.title.none.fl_str_mv Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
Caracterización fenotípica y genotípica de Staphylococcus spp con resistencia a meticilina en pollos comerciales
title Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
spellingShingle Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
Cotaquispe, Rony
methicillin-resistant Staphylococcus aureus
methicillin-resistant coagulase positive staphylococci
resistance genes
Staphylococcus aureus resistente a la meticilina
estafilococos coagulasa positivos a la meticilina resistente
genes de resistencia
title_short Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
title_full Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
title_fullStr Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
title_full_unstemmed Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
title_sort Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
dc.creator.none.fl_str_mv Cotaquispe, Rony
Sarmiento, Ruth
Lovón, Stephane
Rodriguez, Jorge
Cotaquispe, Rony
Sarmiento, Ruth
Lovón, Stephane
Rodriguez, Jorge
author Cotaquispe, Rony
author_facet Cotaquispe, Rony
Sarmiento, Ruth
Lovón, Stephane
Rodriguez, Jorge
author_role author
author2 Sarmiento, Ruth
Lovón, Stephane
Rodriguez, Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv methicillin-resistant Staphylococcus aureus
methicillin-resistant coagulase positive staphylococci
resistance genes
Staphylococcus aureus resistente a la meticilina
estafilococos coagulasa positivos a la meticilina resistente
genes de resistencia
topic methicillin-resistant Staphylococcus aureus
methicillin-resistant coagulase positive staphylococci
resistance genes
Staphylococcus aureus resistente a la meticilina
estafilococos coagulasa positivos a la meticilina resistente
genes de resistencia
description The study aimed to identify Staphylococcus spp with phenotypes and genotypes of methicillin resistance, as well as to identify variants of the coa + gene polymorphism in S. aureus. In total, 97 strains of Staphylococcus spp isolated from chickens were used and antibiograms and multiplex PCR, conventional PCR and sequencing tests were carried out. The phenotypic results indicate the presence of resistance to 2 MRSA and 26 SCN resistant to methicillin (cefoxitin) and 2 S. aureus and 67 SCN with presumed resistance to methicillin (oxacillin) in the antibiograms. Using PCR, the mecA gene responsible for methicillin resistance was identified in 24 strains: 2 MRSA and 22 SCNRM. In addition, 4 S. aureus genotypes with variants of the coagulase gene polymorphism were identified. The results suggest a constant genetic activity, the appearance of clones and the expression of genes for resistance to the main antibiotics faced by Staphylococcus spp.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20395
10.15381/rivep.v32i3.20395
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20395
identifier_str_mv 10.15381/rivep.v32i3.20395
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/20395/16825
dc.rights.none.fl_str_mv Derechos de autor 2021 Rony Cotaquispe, Ruth Sarmiento, Stephane Lovón, Jorge Rodriguez
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2021 Rony Cotaquispe, Ruth Sarmiento, Stephane Lovón, Jorge Rodriguez
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 3 (2021); e20395
Revista de Investigaciones Veterinarias del Perú; Vol. 32 No. 3 (2021); e20395
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238235362820096
score 13.9517765
Phenotypic and genotypic characterization of Staphylococcus spp with resistance to methicillin in commercial chickens
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares